A5045944491- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
- Infective Endocarditis Diagnosis and Management
- Antifungal resistance and susceptibility
- Microbial infections and disease research
- Diphtheria, Corynebacterium, and Tetanus
- Pneumonia and Respiratory Infections
- Mechanical Circulatory Support Devices
- Fungal Infections and Studies
- Escherichia coli research studies
- Neonatal and Maternal Infections
- Cerebrospinal fluid and hydrocephalus
- Infectious Encephalopathies and Encephalitis
- Genomics and Rare Diseases
- Aortic Disease and Treatment Approaches
- Surgical Simulation and Training
- Dementia and Cognitive Impairment Research
- Plant Pathogens and Fungal Diseases
- Rabies epidemiology and control
- Cancer therapeutics and mechanisms
- Erythropoietin and Anemia Treatment
University Hospital Heidelberg
2015-2024
Heidelberg University
2011-2024
Brigham and Women's Hospital
2022
Harvard University
2022
Institute of Medical Microbiology and Hygiene
2021
California Department of Education
2021
Universidade Federal de Santa Maria
2020
Instituto Federal Farroupilha
2020
Charité - Universitätsmedizin Berlin
2012
Anticipating the burden of intracerebral haemorrhage is crucial for proactive management and building resilience against future health challenges. Prior forecasts are based on population demography to a lesser extent epidemiological trends. This study aims utilise selected modifiable risk factors socio-demographic indicators forecast incidence mortality in Europe between 2019 2050.
We report a case of resistance development toward cefiderocol in patient with intra-abdominal and bloodstream infections caused by carbapenemase-producing Enterobacter cloacae within 21 days therapy. Whole genome sequencing revealed heterogeneous mutations the cirA gene, encoding catecholate siderophore receptor, conferring phenotypic to cefiderocol.
Cefiderocol is a promising novel siderophore cephalosporin for the treatment of multidrug-resistant Gram-negative bacilli and with stability against degradation by metallo-β-lactamases. Nonetheless, emergence cefiderocol in metallo-β-lactamase-producing Enterobacterales during therapy has been reported on more than one occasion. To understand underlying mechanisms factors facilitating resistance development, we conducted an vitro evolution experiment using clinical E. cloacae isolates via...
Sepsis is a major cause of mortality in hospitalized patients worldwide, with lethality rates ranging from 30 to 70 %. caused by variety different pathogens, and rapid diagnosis outstanding importance, as early adequate antimicrobial therapy correlates positive clinical outcome. In recent years, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) fingerprinting has become powerful tool microbiological diagnostics. The direct identification...
Background: The detection of pathogens causing infections by conventional diagnostic methods can be challenging and next-generation sequencing (NGS) technology offers a promising alternative method. In this study, we evaluated the performance real-time metagenomic (rt-mNGS) for in respiratory samples. Method: We used rt-mNGS, using Seqstant LiveGene Analysis platform, on 335 samples comparison to culture results. Results: observed an overall good concordance 71.64% (240/335) methods. rt-mNGS...
<h3>Importance</h3> <i>Staphylococcus aureus</i>is one of the leading causes infections in neonatal intensive care units (NICUs). Most studies this patient group focus on methicillin-resistant<i>S aureus</i>or outbreak setting, whereas data for methicillin-susceptible<i>S aureus</i>are limited. <h3>Objectives</h3> To identify risk factors for<i>S aureus</i>colonization and hospitalized newborns to investigate<i>S aureus</i>transmission its dynamics a nonoutbreak setting. <h3>Design, Setting,...
While total laboratory automation (TLA) is well established in medicine, only a few microbiological laboratories are using TLA systems. Especially terms of speed and accuracy, working with expected to be superior conventional microbiology. We compared 35,564 urine cultures without incubation processing BD Kiestra for 6-month period each retrospectively. Sixteen thousand three hundred thirty-eight samples were analyzed the pre-TLA 19,226 TLA. Sixty-two percent (n = 10,101/16338) processed 68%...
Abstract Staphylococcus aureus is one of the major pathogens causing community—and healthcare-acquired infections. The presence virulence factor Panton–Valentine leukocidin (PVL) associated with recurrent infection and clinical severity generally regarded as a feature community associated-methicillin-resistant (MRSA). To date, focus PVL-positive MRSA in hospitalized patients has been on outbreaks. We aimed to investigate whether penetrated community-hospital barrier by determining prevalence...
Dalbavancin is emerging as a promising alternative in the ambulant treatment of gram-positive infections that require long-term antibiotic such osteomyelitis, prosthetic joint infections, and endocarditis. The aim current study was to develop validate simple, rapid, cost-effective high-performance liquid chromatography-ultraviolet spectrometry (HPLC-UV) method for quantification dalbavancin. Sample clean-up included protein precipitation protocol, followed by chromatographic separation on...
Abstract Objectives VRE are listed, by the WHO, among leading resistant pathogens causing greatest public concern; hence spread and transmission of VRE, especially in hospitalized patients, need to be monitored. Despite advancements typing methods since implementation WGS for outbreak investigations, data interpretation, vancomycin-resistant Enterococcus faecium (VREfm) an endemic setting, remains challenging. In this study we explored potential added benefit incorporating patient movement...
Septic shock substantially alters the pharmacokinetic properties of β-lactams with a subsequently high risk insufficiently low serum concentrations and treatment failure. Considering their (PK)/pharmacodynamic (PD) index, prolonged infusions (PI) extend time that unbound fraction drug remains above minimal inhibitory concentration MIC (ft >MIC) may improve patient survival. The present study is monocentric, retrospective before-and-after analysis septic patients treated β-lactams....
ObjectivesTo assess, whether S. aureus nasal colonization is a risk factor for infections in patients with durable ventricular assist device (VAD).MethodsProspective, single-centre, cohort study (i) ascertaining status of admitted VAD-implantation and detecting time to first episode VAD-specific or -related infection according International Society Heart Lung Transplantation criteria during follow-up (ii) comparing whole genomes from baseline later infection.ResultsAmong 49 (17 colonized, 32...
Background: Timing of surgery remains controversial in patients with infective endocarditis and stroke. Guidelines on suggest delaying for up to 4 weeks. However, early heart failure due progression the infection or recurrent septic embolism, urgent becomes imperative. Methods: Out 688 who were surgically treated left-sided endocarditis, 187 presented preoperative neurological events. The date cerebral stroke onset was documented 147 patients. stratified according timing surgery: 61 group...
Increasing evidence suggests that dalbavancin is an effective long-term treatment for ventricular assist device (VAD) infections, with various prolonged dosing regimens currently in use. This retrospective study aimed to assess pharmacokinetics VAD patients and identify optimal, feasible suppressive outpatient therapy. Data from Heidelberg University Hospital’s register were analyzed using non-linear mixed-effects modeling pharmacokinetic analysis simulations (Lixoft®). The probability of...
Abstract The role of outpatient clinics as a potential transmission ground for multidrug-resistant organisms has not been adequately investigated. Here, we report cluster blaKPC-2-positive Enterobacter cloacae among patients treated in highly frequented department.
There is a growing interest in the rapid genotypic identification of antimicrobial resistance (AMR). In routine diagnostics, we detected multiple KPC-positive Escherichia coli (KPC-Ec) with discordant phenotypic meropenem susceptibility from single patient's blood cultures, which prompted more thorough investigation.We investigated potential clinical relevance of, and mechanism behind, KPC-Ec.WGS was used to perform comparative analysis isolates' genetic characteristics their blaKPC-2 locus....
Mycoplasma canis is an opportunistic bacterial pathogen that may colonize dogs and cattle. In the present case, we report for first time isolation of M. from a wound tissue specimen 62-year-old woman after dog bite.
Abstract Objectives Increasing spread of resistance could jeopardize the use antifolates against MRSA infections. Methods We compared prevalence phenotypic trimethoprim/sulfamethoxazole in 20 534 clinical Staphylococcus aureus isolates (19 096 MSSA and 1438 MRSA) non-redundant patients at Heidelberg University Hospital over 8 years performed WGS on trimethoprim/sulfamethoxazole-resistant MRSA. Results From 2012 to 2019, (674/19 096; 3.5%) ranged between 1.5% 7.2% (135/1438; 9.4%) 0.5% 20.2%,...
Trimethoprim-sulfamethoxazole (SXT) is a valuable second-line antimicrobial agent to treat methicillin-resistant Staphylococcus aureus infections. Discrepancies between various antibiotic susceptibility testing (AST) methods for SXT in S. have been described. Here, we describe hemin-inducible heteroresistance phenotype aureus. We compared the results of Vitek 2 AST on set 95 clinical isolates with broth microdilution, disk diffusion using standard Mueller-Hinton agar, and agar supplemented...