A5029764358- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Ion channel regulation and function
- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Amyotrophic Lateral Sclerosis Research
- Coenzyme Q10 studies and effects
- Muscle metabolism and nutrition
- GDF15 and Related Biomarkers
- Neurogenetic and Muscular Disorders Research
- Healthcare and Venom Research
- Cerebral Palsy and Movement Disorders
- Thermoregulation and physiological responses
- Asthma and respiratory diseases
- Genetics, Aging, and Longevity in Model Organisms
- Nutrition and Health in Aging
- Genetics and Physical Performance
- Antioxidant Activity and Oxidative Stress
- Heat shock proteins research
- Cardiac electrophysiology and arrhythmias
- Cardiovascular and exercise physiology
- Telomeres, Telomerase, and Senescence
- DNA Repair Mechanisms
University of Liverpool
2015-2024
Versus Arthritis
2021-2024
Université de Yaoundé I
1995
University of Alabama at Birmingham
1995
New York Blood Center
1995
Abstract Age‐associated loss of muscle mass and function (sarcopenia) has a profound effect on the quality life in elderly. Our previous studies show that CuZnSOD deletion mice ( Sod1 −/− mice) recapitulates sarcopenia phenotypes, including elevated oxidative stress accelerated atrophy, weakness, disruption neuromuscular junctions (NMJs). To determine whether initiated neurons adult is sufficient to induce we treated young (2‐ 4‐month‐old) Sod1flox/SlickHCre with tamoxifen generate i...
Disruption of neuromuscular junctions and denervation some muscle fibers occurs in ageing skeletal contribute to loss mass function. Aging is associated with mitochondrial dysfunction redox homeostasis potentially through increased generation reactive oxygen species (ROS). No specific link between ROS has been defined ageing. To address this, we have examined the effect experimental all fibers, or only a proportion mouse tibialis anterior (TA) on peroxide generation. Transection peroneal...
Previous studies have shown a significant increase in the mitochondrial generation of hydrogen peroxide (H2O2) and other peroxides recently denervated muscle fibers. The mechanisms for these how responds to are not fully established. aim this work was determine effect denervation on content proteins that may contribute release responses generation. Denervation tibialis anterior (TA) extensor digitorum longus (EDL) muscles mice achieved by surgical removal small section peroneal nerve prior...
ObjectiveThe anterior cruciate ligament transection (ACLT) rabbit osteoarthritis (OA) model confers permanent knee instability and induces joint degeneration. The degeneration process is complex, but includes chondrocyte apoptosis OA-like loss of cartilage integrity. Previously, we reported that activation a volume-sensitive Cl− current (ICl,vol) can mediate cell shrinkage in articular chondrocytes. Our objective was therefore to investigate whether ICl,vol activated the early stages ACLT OA...
Abstract Whole-body knock-out of Cu,Zn superoxide dismutase (Sod1KO) results in accelerated, age-related loss muscle mass and function associated with neuromuscular junction (NMJ) breakdown similar to sarcopenia. In order determine whether altered redox motor neurons underlies this phenotype, an inducible neuron-specific deletion Sod1 (i-mnSod1KO) was compared wild-type (WT) mice different ages (adult, mid-age, old) whole-body Sod1KO mice. Nerve oxidative damage, neuron numbers structural...
What is the central question of this study? Can modulation inducible NO synthase reduce pain behaviour and pro-inflammatory cytokine signalling in a rat model neuropathic pain? main finding its importance? Nitric oxide synthase-based therapies could be effective for treatment peripheral pain.Peripheral (PNP), resulting from injury to or dysfunction nerve, major health problem that affects 7-8% population. It inadequately controlled by current drugs characterized hypersensitivity, which...
To determine the role of denervation and motor unit turnover in age-related increase skeletal muscle oxidative stress, hydrogen peroxide (H2O2) specific, genetically-encoded, fluorescent cyto-HyPer2 probe was expressed mouse anterior tibialis (AT) compared with ex vivo measurements mitochondrial oxidant generation. Crush peroneal nerve induced increased generation, measured permeabilised AT fibers intra vital confocal microscopy fluorescence showed cytosolic H2O2 a sub-set (~24%) individual...
The synovium secretes synovial fluid, but is also richly innervated with nociceptors and acts as a gateway between avascular joint tissues the circulatory system. Resident fibroblast-like synoviocytes' (FLS) calcium-activated potassium channels (KCa) change in activity arthritis models this correlates FLS activation.To investigate activation an vitro model of inflammatory arthritis; 72 h treatment cytokines TNFα IL1β.FLS cells were isolated from rat membranes. We analyzed global changes mRNA...
Abstract Motor unit remodelling involving repeated denervation and re-innervation occurs throughout life. The efficiency of this process declines with age contributing to neuromuscular deficits. This study investigated differentially expressed genes (DEG) in muscle following peroneal nerve crush model motor C57BL/6 J mice. Muscle RNA was isolated at 3 days post-crush, libraries were generated using poly-A selection, sequenced analysed gene ontology pathway tools. Three hundred thirty-four...
In humans, skin is a primary thermoregulatory organ, with vasodilation leading to rapid body cooling, whereas in Rodentia the tail performs an analogous function. Many thermodetection mechanisms are likely be involved including transient receptor potential vanilloid-type 4 (TRPV4), ion channel thermosensitive properties. Previous studies have shown that TRPV4 vasodilator by local action blood vessels, so here, we investigated whether constitutive activity affects Mus muscularis vascular tone...
Introduction Hypertonic media causes cells to shrink due water loss through aquaporin channels. After acute shrinkage, either regulate their volume or, alternatively, undergo a number of metabolic changes which ultimately lead cell death. In many types, hypertonic shrinkage is followed by apoptosis. Due the complex 3D morphology skeletal muscle and difficulty in obtaining isolated human tissue, we have begun regulation studies using line TE671RD. this study investigated whether challenge...
Skeletal muscle generates superoxide during contractions, which is converted to hydrogen peroxide (H2O2). H2O2 has been proposed activate signalling pathways and transcription factors that regulate adaptive responses exercise, but the concentration required oxidize key redox-sensitive proteins in vitro much higher than typical intracellular levels seen after exercise. We hypothesized 2-Cys-peroxiredoxins (PRDX), rapidly presence of physiological concentrations H2O2, serve as intermediary...
In addition to the established intracellular production of H 2 0 by skeletal muscle it is also produced extracellularly and studies have shown that levels contribute pathophysiology age‐related loss mass function [1, 2]. Furthermore, externally applied O , inhibits neurotransmission an undefined mechanism [3]. Despite considerable advancement our understanding sources, sinks functions within fibres, there been no previous reports how transported into fibres. Recent evidence in other systems...
Age‐related loss of muscle mass and function occurs over a substantial portion later life greatly influences the quality older people. The causes this dysfunction are unknown, but remodelling motor units denervation evident in muscles old mice. Full caused elevations rate mitochondrial H 2 0 production. Data from our laboratory have demonstrated that was elevated at 3 days post‐denervation remained significantly for 10 days, compared with sham controls. Further work has shown increase O is...
Abstract In humans the skin is a primary thermoregulatory organ, with vasodilation leading to rapid body cooling, whereas in Rodentia tail performs an analogous function. Many thermodetection mechanisms are likely be involved including transient receptor potential vanilloid-type 4 (TRPV4), widely distributed ion channel both mechanical and thermosensitive properties. Previous studies have shown that TRPV4 can act as vasodilator by local action blood vessels, this study, we investigated...
Loss of skeletal muscle mass and function are critical factors in the development frailty although mechanisms by which this loss occurs poorly understood. The number senescent cells increases with age rodents (Krishnamurthy et al ., 2004) primates (Kreiling 2011) it is proposed that presence these plays a role age‐related dysfunction. We hypothesise resident satellite become exert detrimental effects on mature fibres via senescence associated secretory phenotype molecules (SASP). Initial...