A5015240122- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Kidney Stones and Urolithiasis Treatments
- Urological Disorders and Treatments
- Nanoplatforms for cancer theranostics
- RNA modifications and cancer
- Pediatric Urology and Nephrology Studies
- Radiation Dose and Imaging
- Ureteral procedures and complications
- Cancer Research and Treatments
- Traumatic Ocular and Foreign Body Injuries
- Cancer-related Molecular Pathways
- Urinary Tract Infections Management
- Cancer Genomics and Diagnostics
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Urinary Bladder and Prostate Research
- Mechanisms of cancer metastasis
- Radiomics and Machine Learning in Medical Imaging
- COVID-19 Impact on Reproduction
- Colorectal Cancer Surgical Treatments
- Surgical Sutures and Adhesives
- Sleep and related disorders
- Pressure Ulcer Prevention and Management
- Digital Radiography and Breast Imaging
Loma Linda University
2016-2024
UCSF Helen Diller Family Comprehensive Cancer Center
2013-2023
University of California, San Francisco
2014-2023
Loma Linda University Health Care
2023
Loma Linda University Children's Hospital
2022
Loma Linda University Medical Center
2017-2022
National Institutes of Health
2018
National Cancer Institute
2018
City College of San Francisco
2013
Stanford University
2010
Abstract RNAi is a powerful tool for target identification and can lead to novel therapies pharmacologically intractable targets such as KRAS. therapy must combine potent siRNA payloads with reliable in vivo delivery efficient inhibition. We used functional “Sensor” assay establish library of siRNAs against RAS pathway genes show that they efficiently suppress their at low dose. This reduces off-target effects enables combination gene knockdown. administered Sensor vitro validated the KRAS...
Abstract Percutaneous nephrolithotomy confers the highest radiation to urologist’s hands compared other urologic procedures. This study compares exposure surgeon’s hand and patient’s body when utilizing three different techniques for needle insertion during renal access. Simulated percutaneous access was performed using a cadaveric patient separate forearm representing hand. Three needle-holding were compared: conventional glove (control), radiation-attenuating glove, novel holder. Five...
To investigate the effect of laser fiber stripping on stone fragmentation and power output.In a benchtop simulation lithotripsy, 20 BegoStone phantoms were positioned within ureteral model irradiated for 10 minutes at 8 Hz 0.8 J. A freshly cleaved 365 μm was used all trials, with half fibers also undergoing stripping. Power output measured 1-minute intervals, beginning an initial prelithotripsy recording 0 minutes. Fiber tips imaged scanning electron microscopy. In single-blinded manner,...
Flat-panel detector C-arms (FCs) are reported to reduce radiation exposure and improve image quality compared with conventional intensifier (CCs). The purpose of this study was compare between three commonly used FCs.
Urologists frequently activate foot pedals in a low-light operating room (OR). Pedal activation conditions poses the potential for incorrect pedal activation, potentially leading to increased radiation exposure, patient burns, or OR fires. This study compares speed, accuracy, dark adaptation, and surgeon preference 4 lighting conditions.
Abstract Background KRAS mutations are present in up to 30% of lung adenocarcinoma cases and associated with poor survival. No effective targeted therapy against is currently available, novel strategies counteract oncogenic signaling needed. Results We used proteomics monitor abundance site-specific phosphorylation a network over 150 upstream downstream effectors H358 cells (KRAS G12C). compared patterns protein regulation following sustained blockade the RAS/ERK module at two different...
You have accessJournal of UrologyStone Disease: Surgical Therapy I1 Apr 2016MP22-14 THE IMPACT OF LASER FIBER STRIPPING ON STONE FRAGMENTATION & POWER OUTPUT Cayde Ritchie, Patrick Yang, Brandon Peplinski, Salim Cheriyan, Samuel Abourbih, and Duane Baldwin RitchieCayde Ritchie More articles by this author , YangPatrick Yang PeplinskiBrandon Peplinski CheriyanSalim Cheriyan AbourbihSamuel Abourbih BaldwinDuane View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.702AboutPDF...
You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology I1 Apr 2016MP58-16 DAMAGE TO POLYTETRAFLUOROETHYLENE-COATED GUIDEWIRES: A POTENTIAL FOREIGN BODY RISK Alison Wong, Wayne Kelln, Danilo Boskovic, Alex Erskine, Andrew Kutzner, Cayde Ritchie, Brian Chung, Samuel Abourbih, Salim Cheriyan, Mohamed Keheila, Jim Shen, Patrick Yang, and D. Duane Baldwin WongAlison Wong More articles by this author , KellnWayne Kelln BoskovicDanilo Boskovic ErskineAlex Erskine...
Abstract Cancer stem cells (CSCs) can cause cancer treatment failures and tumor recurrence due to their cell-like properties, such as the unlimited self-renewal, tumorigenicity, chemo-resistance. Ras is most common oncogene in human cancers, but its roles properties have not been convincingly demonstrated. With a high degree of sequence homology well sets downstream effectors upstream affecters, three isoforms Ras, N-, H- K-Ras, long assumed be functionally redundant. However, knockout N- or...
Abstract The current landscape of druggable disease targets represents only ~10% the human genome. Among those that are frequently mutated in cancer yet remain undruggable by conventional small molecules Ras family GTPases. Somatic mutations KRAS occur 35% colorectal cancers, 16% lung cancers and 61% pancreatic cancers. Common lock enzyme active GTP-bound state, which leads to constitutive activation downstream effector modules such as MAPK, PI3K, Ral, Rac Rho, promote tumor growth,...
You have accessJournal of UrologyCME1 Apr 2023MP70-15 PRIMARY VESICOURETERAL REFLUX IN A MODERN COHORT: WHAT ARE THE RISK FACTORS FOR URINARY TRACT INFECTIONS? Daniel Wilkinson, Matthew Buell, Kai Wen Cheng, Cayde Ritchie, Levi Miles, Jocelyn Huang, Ala’a Farkouh, David Chamberlin, and Joshua Chamberlin WilkinsonDaniel Wilkinson More articles by this author , BuellMatthew Buell ChengKai Cheng RitchieCayde Ritchie MilesLevi Miles HuangJocelyn Huang FarkouhAla’a Farkouh ChamberlinDavid...
You have accessJournal of UrologyCME1 Apr 2023MP53-16 TELEMEDICINE HERE TO STAY? KEY INDICATORS PATIENT SATISFACTION Gideon Harianja, Michael Reimer, Cayde Ritchie, Matthew Buell, Akin S. Amasyali, Kai Wen Cheng, Catherine Chen, David Chamberlin, and Joshua Chamberlin HarianjaGideon Harianja More articles by this author , ReimerMichael Reimer RitchieCayde Ritchie BuellMatthew Buell AmasyaliAkin Amasyali ChengKai Cheng ChenCatherine Chen ChamberlinDavid ChamberlinJoshua View All Author...
You have accessJournal of UrologyCME1 Apr 2023MP53-03 UROLOGIC RISK FACTORS FOR URINARY TRACT INFECTION AND ALLOGRAFT FAILURE IN PEDIATRIC RENAL TRANSPLANTATION Vinicius Seidel, Matthew Buell, Kai Wen Cheng, Cayde Ritchie, Adam Fisher, David Chamberlin, and Joshua Chamberlin SeidelVinicius Seidel More articles by this author , BuellMatthew Buell ChengKai Cheng RitchieCayde Ritchie FisherAdam Fisher ChamberlinDavid ChamberlinJoshua View All Author...
<p>Analysis of shRNA and endogenous miRNA representation.</p>
Supplemental Figure and Table Legends from Development of siRNA Payloads to Target <i>KRAS</i>-Mutant Cancer
<p>Sequences of Sensor identified potent sh- and siRNAs targeting human mouse Ras effector genes.</p>
<p>Supplemental Figure S1. Sensor-based generation of a functionally validated sh/siRNA library targeting Ras pathway genes. S2. Validation Sensor siRNAs components in the MAPK pathway. S3. Dose dependent off-target effects sh/siRNAs. S4. siRNA pooling effects. S5. In vivo fluorescence reporter system. Table Correlation reads and score.</p>
Supplemental References from Development of siRNA Payloads to Target <i>KRAS</i>-Mutant Cancer
Supplemental Methods from Development of siRNA Payloads to Target <i>KRAS</i>-Mutant Cancer