A5110951651- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Cholesterol and Lipid Metabolism
- Hormonal and reproductive studies
- Ovarian function and disorders
- Pain Mechanisms and Treatments
- Seed Germination and Physiology
- Drug Transport and Resistance Mechanisms
- Hypothalamic control of reproductive hormones
- Neural dynamics and brain function
- Bee Products Chemical Analysis
- Growth Hormone and Insulin-like Growth Factors
- Neuropeptides and Animal Physiology
- Circadian rhythm and melatonin
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- Olfactory and Sensory Function Studies
- Lipid Membrane Structure and Behavior
- Hormonal Regulation and Hypertension
- Neuroscience and Neural Engineering
- Retinoids in leukemia and cellular processes
- EEG and Brain-Computer Interfaces
- Regulation of Appetite and Obesity
- Neural Networks and Applications
- Adipose Tissue and Metabolism
Université Clermont Auvergne
2006-2022
Inserm
2012-2022
Clermont Université
2006-2022
Neuro-Dol
2016-2021
Centre National de la Recherche Scientifique
1986-2017
Centre de Recherche en Nutrition Humaine d'Auvergne
2016
Ministry of Infrastructure
2015
Assaf Harofeh Medical Center
2011
Laboratoire de Biologie du Développement
1994-2010
Genetique Reproduction and Developpement
2006-2007
Abstract It is well known that leptin, the ob gene product, involved in regulation of food intake and thermogenesis. Recent studies also demonstrate leptin may be able to modulate functions cells nonspecific immune response such as phagocytosis secretion cytokines by macrophages. This prominent implication polymorphonuclear neutrophils (PMNs) infectious suggested a possible role modulator PMN functions. We detected receptor on membrane immunocytochemistry with an anti-leptin receptor. Using...
We have isolated vesicular structures from mouse epididymal fluid, referred to as epididymosomes. Epididymosomes a roughly spherical aspect and bilayer membrane, they are heterogeneous in size content. They originate the epithelium, notably caput region, emitted lumen by way of apocrine secretion. characterized their membranous lipid profiles cauda epididymidal fluid samples found that epididymosomes were particularly rich sphingomyelin (SM) arachidonic acid. The proportion SM increased...
Human gut microbiota harbors numerous metabolic properties essential for the host's health. Increased intestinal transit time affects a part of population and is notably observed with human aging, which also corresponds to modifications microbiota. Thus we tested compositional changes induced by an increased simulated in vitro.The vitro system, Environmental Control System Intestinal Microbiota, was used simulate environmental conditions 3 different anatomical parts colon continuous process....
BACKGROUND: Cryopreservation/thawing of bovine spermatozoa induces a reduction in cell viability and is possibly associated with form programmed death that we previously named 'apoptosis-like phenomenon'. METHODS: In this study, specified, by flow cytometry, the moment appearance some characteristics apoptosis during cryopreservation process. We also studied presence and/or activation sperm cells specific proteins involved somatic western blot fluorimetry. RESULTS: A decrease mitochondrial...
Oxysterol nuclear receptors liver X receptor (LXR)alpha and LXRbeta are known to regulate lipid homeostasis in cells exposed high amounts of cholesterol and/or fatty acids. In order elucidate the specific redundant roles LXRs testis, we explored reproductive phenotypes mice deficient LXRalpha, LXRbeta, both, which only lxralpha;beta-/- infertile by 5 months age. We demonstrate that LXRalpha-deficient had lower levels testicular testosterone correlated with a higher apoptotic rate germ cells....
Seminal fluid inhibits sperm capacitation mainly because of its high cholesterol content. Prostasomes are the main source in seminal fluid. They known to have numerous protective properties and able transfer proteins lipids spermatozoa, but their impact on acrosome reaction (AR) is not yet well understood. The aim this study was determine effects prostasomes human AR. After 80% Percoll selection, freshly ejaculated spermatozoa were incubated for 3 h under capacitating conditions with...
Cryopreservation of ovarian tissue can be used to preserve the fertility patients who are about receive treatment(s) that could compromise their future function. Here we evaluate effectiveness a vitrification protocol by carrying out systematic comparison with conventional slow-freezing method on human tissue.Human samples (mean age 28.0 ± 1.1 years) were processed in parallel for each cryopreservation procedure: and slow-freezing. Following warming/thawing, histological observations TUNEL...
To try to explain the infertility of chromosomal translocation carrier patients, we compared expression two markers apoptosis in sperm patients and fertile donors, studied meiotic segregation ejaculated these carriers.Twenty semen samples carriers, [reciprocal (n=14) Robertsonian translocations (n=6)], were with donors (n=20). Different tests applied: annexin V binding assay; terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL); fluorescence situ...
Recommendations for cardiovascular disease prevention advocate lowering both cholesterol and low-density lipoprotein systemic levels, notably by statin intake. However, statins are the subject of questions concerning their impact on male fertility. This study aimed to evaluate, a prospective pilot assay, efficacy toxicity decrease blood induced atorvastatin semen quality sexual hormone levels healthy, normocholesterolaemic normozoospermic men. Atorvastatin (10 mg daily) was administrated...
The uterus is an organ where lipid distribution plays a critical role for its function. Here we show that nuclear receptor oxysterols LXRβ prevents accumulation of cholesteryl esters in mouse myometrium by controlling expression genes involved cholesterol efflux and storage (abca1 abcg1). Upon treatment with LXR agonist mimics activation oxysterols, these target was increased wild-type mice, whereas under basal conditions, lxrα;β-/- mice exhibited marked decrease abcg1 accumulation. This...
The composition of the mitochondrial inner membrane and uncoupling protein [such as adenine nucleotide translocator (ANT)] contents are main factors involved in energy-wasting proton leak. This leak is increased by glucocorticoid treatment under nonphosphorylating conditions. aim this study was to investigate mechanisms glucocorticoid-induced evaluate consequences more physiological conditions (between states 4 3). Isolated liver mitochondria, obtained from dexamethasone-treated rats (1.5...
Irritable Bowel Syndrome (IBS) and Inflammatory Disease (IBD) are related gastrointestinal disorders characterized by abdominal pain associated with colonic hypersensitivity (CHS). Studies in humans have reported an abnormal colonization of Adherent-Invasive E. coli (AIEC) the ileum Crohn's disease (CD) patients overexpression bacterial colonizing receptor CEACAM6. The aim present study was to investigate whether AIEC reference strain LF82 could induce intestinal impairment during infectious...
Testes of most male mammals present the particularity being externalized from body and are consequently slightly cooler than core temperature (4–8°C below). Although, hypothermia testis is known to increase germ cells apoptosis, little about underlying molecular mechanisms, including cold sensors, transduction pathways, apoptosis triggers. In this study, using a functional knockout mouse model menthol receptors, dubbed transient receptor potential melastatine 8 (TRPM8) channels, we found...
Cryopreservation-induced stress may result in membrane injury with consequent decrease of sperm motility and fertilizing capacity. This study has investigated the relationship between human spermatozoa tolerance to cryopreservation loss plasma asymmetry, especially translocation phosphatidylserine (PS) from inner outer leaflet. The prospective was performed on semen samples 31 men. Conventional characteristics 20 were analysed, before after as well externalization PS assessed by annexin...
Prostasomes, vesicles present in human semen, are known to play a role male fertility. However, the mechanisms involved still poorly understood. The study looks at direct influence of different concentrations prostasomes on sperm function conditions supporting capacitation vitro. Five million Percoll selected spermatozoa were incubated for 3 h 370C under an atmosphere 5% CO2 air, 100 µl Biggers Whitten Whittingham's medium (BWW) containing polyvinyl alcohol (PVA, 1 mg/ml) and bovine serum...