A5032835934- SARS-CoV-2 and COVID-19 Research
- Toxic Organic Pollutants Impact
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Effects and risks of endocrine disrupting chemicals
- COVID-19 diagnosis using AI
- COVID-19 epidemiological studies
- Environmental Toxicology and Ecotoxicology
- Vaccine Coverage and Hesitancy
- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
- Air Quality and Health Impacts
- bioluminescence and chemiluminescence research
- Antimicrobial Resistance in Staphylococcus
- Elbow and Forearm Trauma Treatment
- Shoulder Injury and Treatment
- Shoulder and Clavicle Injuries
- Retinoids in leukemia and cellular processes
- Traumatic Ocular and Foreign Body Injuries
- Estrogen and related hormone effects
- Pharmaceutical studies and practices
- Atrial Fibrillation Management and Outcomes
- Heparin-Induced Thrombocytopenia and Thrombosis
- Per- and polyfluoroalkyl substances research
- Mechanical Circulatory Support Devices
Palacký University Olomouc
2024
University of Ostrava
2020-2024
Masaryk University
2000-2009
Veterinary Research Institute
2004
We investigated the validity of claims healthy vaccinee effect (HVE) in COVID-vaccine studies by analyzing associations between all-cause mortality (ACM) and COVID-19 vaccination status.
Antigen testing for SARS-CoV-2 (AGT) is generally considered inferior to RT-PCR in terms of sensitivity. However, little known about the infectiousness positive patients who pass undetected by AGT. In a screening setting mildly symptomatic or asymptomatic with high COVID-19 prevalence (30–40%), 1141 were tested using one five AGTs and RT-PCR. Where results differed, virus viability samples was on cell culture (CV-1 cells). The test battery included JOYSBIO, Assure Tech, SD Biosensor,...
Toxic effects of many persistent organic pollutants (e.g., polychlorinated biphenyls or dibenzo-p-dioxins and furans) are mediated via the aryl hydrocarbon receptor (AhR). Although polycyclic aromatic hydrocarbons (PAHs) their derivatives also activate AhR, toxic remain to be fully elucidated. In present study, we used in vitro H4IIE-luc transactivation cell assay investigate cytotoxicity potencies AhR by 29 individual PAHs N-heterocyclic (aza-PAHs). The aza-PAHs were found significantly...
Cellular immunity against SARS-CoV-2 is an important component of the immune response to virus. At present, two such tests based on interferon-gamma release (interferon-γ assays, IGRAs) are available—Quan-T-Cell by EUROIMMUN and T-SPOT.COVID Oxford Immunotec. In this paper, we compared results these in 90 subjects employed at Public Health Institute Ostrava who had previously undergone COVID-19 infection or were vaccinated that disease. To best our knowledge, first head-to-head comparison...
The toxic effects of photoproducts formed upon the photolysis 2- and 4-chlorophenol (CP) frozen solutions in polycrystalline ice phase were determined with a bacterial luminescence test (Vibrio fisheri), vitro biomarker assay for dioxin-like (inductions AhR-dependent luciferase H4IIE-luc cells) compared to products same photoreaction aquatic phase. Coupling samples (3'-chlorobiphenyl-2,4'-diol 3-chlorobiphenyl-2,2'-diol from 2-CP 5-chlorobiphenyl-2,4'-diol 4-CP photolysis) found be more V....
Abstract Humic substances (HS) are ubiquitous in the environment. However, some studies indicate that HS could induce direct adverse effects on human health and hormone‐like fish, amphibians, invertebrates. In this study we investigated a possible biochemical mechanism of toxicity via activation aryl hydrocarbon receptor (AhR). AhR mediates toxic biological environmental contaminants such as 2,3,7,8‐tetrachlorodibenzo‐ p ‐dioxin (TCDD), but number structurally diverse compounds has also been...
TF, ZK, and JJ are members of the Association Microbiologists, Immunologists, Statisticians (Sdruzeni mikrobiologu, imunologu a statistiku) in Czech Republic. They have, however, never received any financial or other incentives that could bias this research have no interests to disclose. Petr Sourek is journalist. The original files acquired from SUKL response FOIA request as well manually created machine readable file available at https://github.com/PalackyUniversity/batch-dependent-safety.
Background Antigen testing for SARS-CoV-2 is considered to be less sensitive than the standard reference method – real-time PCR (RT-PCR). It has been suggested that many patients with positive RT-PCR 'missed' by antigen might non-infectious.
Many studies reported good performance of nasopharyngeal swab-based antigen tests for detecting SARS-CoV-2-positive individuals; however, independently evaluating the quality utilizing anterior nasal swabs or saliva are still rare, although such widely used mass testing. In our study, sensitivities, specificities and predictive values seven detection SARS-CoV-2 (one using swabs, two four saliva) were evaluated. a setting high-capacity testing center, quantitative PCR (qPCR) taken and, at...
Abstract Retinoids are known to regulate important processes such as differentiation, development, and embryogenesis. Some effects, malformations in frogs or changes metabolism of birds, could be related disruption the retinoid signaling pathway by exposure organic contaminants. A new reporter gene assay has been established for evaluation modulation individual chemicals environmental samples. The bioassay is based on pluripotent embryonic carcinoma cell line P19 stably transfected with...
Abstract Background Anticoagulation during extracorporeal membrane oxygenation (ECMO) might still lead to severe bleeding complications. Heparin is the most frequently used anticoagulant, but novel drugs could be promising. Argatroban a new alternative heparin. To date, no robust studies have confirmed clear superiority of argatroban (AG) over heparin, although it has some advantages and may safer. Study Design Methods An observational study was conducted in all adult veno‐venous ECMO...
Background and Aims: Elderly nursing home residents are especially prone to a severe course of SARS-CoV-2 infection. In this study, we aimed investigate the complex immune response after vaccination depending on convalescence status vaccine. Methods: Sampling took place in September–October 2021. IgG antibodies against spike protein nucleocapsid protein, titer virus neutralization delta (on subset patients) omicron, cellular immunity (interferon-gamma release assay) were tested vaccinated...
Data on the effectiveness of arthroscopic arthrolysis and extraction osteosynthetic material after osteosynthesis proximal humerus in patients with persisting problems are rare insufficient. In this study, we performed fixation screws, and, where protruding, nail 34 12 months using an intramedullary nail. The treatment was assessed Constant–Murley shoulder score forward flexion difference between treated arm contralateral one. A median increase 16 points CMS 30 degrees reduction recorded...
To this date, there are no recommendations for personalized stress ulcer prophylaxis (SUP) in critical care that would take the patient's individual genetic predispositions into account. Of drugs used purpose, proton pump inhibitors (PPIs) first-choice intensive unit patients. The degradation of is mediated by cytochrome P450 (CYP) enzymes; particular, CYP2C19 and, to a lesser extent, CYP3A4 involved. Expression and metabolic activity of, namely in, significantly affected single nucleotide...