A5000406410- Connexins and lens biology
- Toxic Organic Pollutants Impact
- Effects and risks of endocrine disrupting chemicals
- Aquatic Ecosystems and Phytoplankton Dynamics
- Antimicrobial agents and applications
- Carcinogens and Genotoxicity Assessment
- Antimicrobial Peptides and Activities
- Biocrusts and Microbial Ecology
- Heat shock proteins research
- Polydiacetylene-based materials and applications
- Diet and metabolism studies
- Nicotinic Acetylcholine Receptors Study
- Marine and coastal ecosystems
- Sperm and Testicular Function
- Synthesis and bioactivity of alkaloids
- Genetic and Kidney Cyst Diseases
- Pharmaceutical and Antibiotic Environmental Impacts
- Animal testing and alternatives
- Berberine and alkaloids research
- Environmental Chemistry and Analysis
- Chemical Reactions and Isotopes
- Adipose Tissue and Metabolism
- Medical and Biological Ozone Research
- Microbial bioremediation and biosurfactants
- Per- and polyfluoroalkyl substances research
Masaryk University
2016-2025
RECETOX
2014-2023
Michigan State University
2008-2015
University of Michigan
2009-2015
Laboratoire d’immunologie intégrative du cancer
2015
University of Iowa
2004
Veterinary Research Institute
2004
Czech Academy of Sciences, Institute of Biophysics
2004
Stockholm University
2004
Czech Academy of Sciences
2004
Abstract We report the structure–activity relationship in antimicrobial activity of linear and branched poly(ethylene imine)s (L‐ B‐PEIs) with a range molecular weights (MWs) (500–12 000). Both L‐ B‐PEIs displayed enhanced against Staphylococcus aureus over Escherichia coli . B‐ L‐PEIs did not cause any significant permeabilization E. cytoplasmic membrane. induced depolarization S. membrane although not. The low MW caused little or no hemolysis while are hemolytic. less cytotoxic to human...
Low molecular weight random copolymers bearing protonated primary amine groups and hydrophobic alkyl in the side chains were synthesized their activities against E. coli , S. aureus human red blood cells, epithelial carcinoma cells (HEp-2) quantified. The mole fraction of (f(alkyl)) length major determinants activities. Against activity was diminished as f(alkyl) increased from 0 to about 0.2, but then enhanced dramatically further. Activity continually with increasing f(alkyl). cytotoxicity...
Hemolysis induced by antimicrobial polymers was examined to gain an understanding of the mechanism polymer toxicity human cells. A series cationic amphiphilic methacrylate random copolymers containing primary ammonium groups as functionality and either butyl or methyl hydrophobic side chains have been prepared radical copolymerization. Polymers with 0−47 mol % in chains, relative total number monomeric units, showed activity but no hemolysis. The 65 27 displayed both hemolytic activity....
Cationic amphiphilic polymethacrylate derivatives (PMAs) have shown potential as a novel class of synthetic antimicrobials. A panel PMAs with varied ratios hydrophobic and cationic side chains were synthesized tested for antimicrobial activity mechanism action. The are to be active against pathogenic bacteria, including drug-resistant Staphylococcus aureus, compared the natural peptide magainin which did not display any same strain. selected 47–63% methyl groups in showed minimum inhibitory...
In the present study, a series of 32 hydroxy- and dihydroxy-polychlorinated biphenyls (OH-PCBs) PCB-derived quinones were prepared evaluated for their in vitro potencies to downregulate gap junctional intercellular communication (GJIC) activate aryl hydrocarbon receptor (AhR) estrogen alpha (ER) well-established liver mammary cell models. The rat epithelial line WB-F344 was used determination GJIC inhibition; AhR-inducing activity determined hepatoma H4IIE.Luc cells stably transfected with...
Perfluoroalkanoates, [e.g., perfluorooctanoate (PFOA)], are known peroxisome proliferators that induce hepatomegaly and hepatocarcinogenesis in rodents, classic non-genotoxic carcinogens inhibit vitro gap-junctional intercellular communication (GJIC). This inhibition of GJIC is to be a function perfluorinated carbon lengths ranging from 7 10.The aim this study was determine if the by PFOA but not perfluoropentanoate (PFPeA) observed F344 rat liver cells also occurs rats vivo mechanisms...
Inhibition of gap junctional intercellular communication (GJIC) and the activation intracellular mitogenic pathways are common hallmarks epithelial derived cancer cells. We previously determined that 1‐methyl not 2‐methyl isomer anthracene, which prominent cigarette smoke components, activated extracellular receptor kinase, inhibited GJIC in WB‐F344 rat liver Using these same cells, we show an immediate upstream response to 1‐methylanthracene was a rapid (<1 min) release arachidonic acid....
Ambient air pollution and smoking are well-documented risk factors for male infertility. Prevalent pollutants cigarette smoke components, polycyclic aromatic hydrocarbons (PAHs), environmental occupational toxicants that act as chemicals disrupting endocrine regulation reproductive potential in males. Testicular gap junctional intercellular communication (GJIC) is critical normal development function of testicular tissue, thus we assessed GJIC a process potentially targeted by PAHs testes....
Microcystin-LR (MC-LR) is a potent hepatotoxin produced by harmful cyanobacterial blooms (CyanoHABs). MC-LR targets highly differentiated hepatocytes expressing organic anion transporting polypeptides OATP1B1 and OATP1B3 that are responsible for hepatocellular uptake of the toxin. The present study utilized an advanced 3D in vitro human liver model Hepoid-HepaRG based on cultivation collagen-matrix embedded multicellular spheroids composed polarized hepatocyte-like cells. 14-d-old cultures...
Toxic effects of many persistent organic pollutants (e.g., polychlorinated biphenyls or dibenzo-p-dioxins and furans) are mediated via the aryl hydrocarbon receptor (AhR). Although polycyclic aromatic hydrocarbons (PAHs) their derivatives also activate AhR, toxic remain to be fully elucidated. In present study, we used in vitro H4IIE-luc transactivation cell assay investigate cytotoxicity potencies AhR by 29 individual PAHs N-heterocyclic (aza-PAHs). The aza-PAHs were found significantly...
Dysregulation of gap junctional intercellular communication (GJIC) has been associated with different pathologies, including cancer; however, molecular mechanisms regulating GJIC are not fully understood. Mitogen Activated Protein Kinase (MAPK)-dependent GJIC-dysregulation have well-established, however recent discoveries implicated phosphatidylcholine-specific phospholipase C (PC-PLC) in the regulation GJIC. What is known how prevalent these two signaling toxicant/toxin-induced...
Abstract Gap junctional intercellular communication (GJIC) is a vital cellular process required for maintenance of tissue homeostasis. In vitro assessment GJIC represents valuable phenotypic endpoint that could be effectively utilized as an integral component in modern toxicity testing, drug screening or biomedical research. However, currently available methods quantifying with higher-throughputs typically require specialized equipment, proprietary software and/or genetically engineered cell...
Humans are exposed to phthalates released from plastics, cosmetics, or food on a daily basis. Phthalates have low acute liver toxicity, but their chronic exposures could induce molecular and cellular effects linked adverse health outcomes, such as tumor promotion diseases. The alternation of gap junctional intercellular communication (GJIC) MAPK-Erk1/2 pathways in progenitor oval cells can disrupt tissue homeostatic mechanisms affect the development severity these outcomes. Our study with 20...