A5011686325- Antimicrobial Peptides and Activities
- Alzheimer's disease research and treatments
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Endoplasmic Reticulum Stress and Disease
- Identification and Quantification in Food
- Environmental DNA in Biodiversity Studies
- Probiotics and Fermented Foods
- Receptor Mechanisms and Signaling
- Primate Behavior and Ecology
- Antimicrobial agents and applications
- Pancreatic function and diabetes
- Advanced Glycation End Products research
- Amphibian and Reptile Biology
- Animal Behavior and Reproduction
- Diabetes Treatment and Management
- Supramolecular Self-Assembly in Materials
- Microbial infections and disease research
- Bacterial Infections and Vaccines
- Bioinformatics and Genomic Networks
- Nicotinic Acetylcholine Receptors Study
- Digital Imaging for Blood Diseases
- Protein Hydrolysis and Bioactive Peptides
- Immune Response and Inflammation
- Drug Transport and Resistance Mechanisms
American Institute of Physics
2024
Rowan University
2012-2023
Stony Brook University
2015-2022
Abstract We report the structure–activity relationship in antimicrobial activity of linear and branched poly(ethylene imine)s (L‐ B‐PEIs) with a range molecular weights (MWs) (500–12 000). Both L‐ B‐PEIs displayed enhanced against Staphylococcus aureus over Escherichia coli . B‐ L‐PEIs did not cause any significant permeabilization E. cytoplasmic membrane. induced depolarization S. membrane although not. The low MW caused little or no hemolysis while are hemolytic. less cytotoxic to human...
Islet amyloidosis by IAPP contributes to pancreatic β-cell death in diabetes, but the nature of toxic species remains elusive. Using concurrent time-resolved biophysical and biological measurements, we define produced during amyloid formation link their properties induction rat INS-1 murine islet toxicity. These globally flexible, low order oligomers upregulate pro-inflammatory markers induce reactive oxygen species. They do not bind 1-anilnonaphthalene-8-sulphonic acid lack extensive...
The pancreatic peptide hormone, amylin, plays a critical role in the control of appetite, and synergizes with other key metabolic hormones such as glucagon-like 1 (GLP-1). There is opportunity to develop potent long-acting analogues amylin or hybrids between these GLP-1 mimetics for treating obesity. To achieve this, interrogation how 37 amino acid engages its complex receptor system required. We synthesized an extensive library peptides profile human sequence, determining disulfide loop,...
Antimicrobial peptides (AMPs) have been an area of great interest, due to the high selectivity these molecules toward bacterial targets over host cells and limited development resistance throughout evolution. The peptide C18G has shown be a selective, broad spectrum AMP with net +8 cationic charge from seven lysine residues in sequence. In this work, Lys were replaced other natural or non-proteinogenic amino acids: arginine, histidine, ornithine, diaminopropionic acid. These changes vary...
ABSTRACT Tryptophan (Trp) is a naturally occurring amino acid, which exhibits fluorescence emission properties that are dependent on the polarity of local environment around Trp side chain. However, this sensitivity also complicates interpretation data. A non‐natural analogue tryptophan, β‐(1‐azulenyl)‐L‐alanine, insensitive to solvent and does not impact structure or characteristics several peptides examined. In study, we investigated effect replacing with β‐(1‐azulenyl)‐L‐alanine in...
The hormone human islet amyloid polypeptide (hIAPP or amylin) plays a role in glucose metabolism, but forms the pancreas type 2 diabetes (T2D) and is associated with β-cell death dysfunction disease. Inhibitors of have therapeutic potential; however, there are no clinically approved inhibitors, mode action existing inhibitors not well understood. Rat IAPP (rIAPP) differs from hIAPP at six positions, does form amyloid, an inhibitor formation by hIAPP. Five differences located within segment...
Human islet amyloid polypeptide (hIAPP) is a hormone secreted from β-cells in the Islets of Langerhans response to same stimuli that lead insulin secretion. hIAPP plays an adaptive role glucose homeostasis but misfolds form insoluble, fibrillar aggregates type II diabetes are associated with disease. Along misfolding pathway, forms species toxic β-cells, resulting reduced β-cell mass. contains strictly conserved disulfide bond between residues 2 and 7, which small loop at N-terminus...
Abstract Arthropods (insects, spiders, etc.) can fulfill major nutritional requirements for primates, particularly in terms of proteins, fats, vitamins, and minerals. Yet, many primate species we know very little about the frequency importance arthropod consumption. Traditional methods prey identification, such as behavioral observations fecal dissections, offer limited taxonomic resolution and, a result, underestimate true diversity. Metabarcoding DNA from samples provides promising but...
Abstract Much of our knowledge diabetes is derived from studies rodent models. An alternative approach explores evolutionary solutions to physiological stress by studying organisms that face challenging metabolic environments. Polar bears eat an enormously lipid‐rich diet without deleterious consequences. In contrast, transgenic rodents expressing the human neuropancreatic polypeptide hormone amylin develop hyperglycemia and extensive pancreatic islet amyloid when fed a high‐fat diet. The...
Pancreatic amyloid formation by the polypeptide IAPP contributes to β-cell dysfunction in type 2 diabetes. There is a 1:1 correspondence between ability of from different species form vitro and susceptibility organism develop Rat non-amyloidogenic differs human at six positions, including three proline replacements: A25P, S28P, S29P. Incorporation these residues into leads analogue that used clinically. The role individual not understood. We examine single double substitutions context IAPP....
Antimicrobial resistance continues to be a major threat world health, with the continued emergence of resistant bacterial strains. peptides have emerged as an attractive option for development novel antimicrobial compounds in part due their ubiquity nature and general lack this class molecules. In work, we analyzed peptide C18G several truncated forms efficacy underlying mechanistic effects sequence truncation. The were screened against standard laboratory strains, further using fluorescence...
Abstract Zombitse‐Vohibasia National Park harbours a species‐rich but understudied lemur community in southwestern Madagascar. Local population estimates are dated or absent for its four sympatric species of Cheirogaleidae: the grey mouse ( Microcebus murinus ), Coquerel's giant Mirza coquereli fat‐tailed dwarf Cheirogaleus medius ) and pale fork‐marked Phaner pallescens ). To provide local density encounter rate these cheirogaleids, we conducted line transect surveys Zombitse sector over...