One of the main challenges for immune checkpoint blockade antibodies lies in malignancies with limited T-cell responses or immunologically "cold" tumors. Inspired by capability fever-like heat inducing an immune-favorable tumor microenvironment, mild photothermal therapy (PTT) is proposed to sensitize tumors inhibition and turn "hot." Here we present a combined all-in-one all-in-control strategy realize local symbiotic photothermal-assisted immunotherapy (SMPAI). We load both near-infrared...

Islet amyloid polypeptide (IAPP, amylin) is the major protein component of islet deposits associated with type 2 diabetes. The lacks a well-defined structure in its monomeric state but readily assembles to form amyloid. Amyloid fibrils formed from IAPP, intermediates generated assembly IAPP amyloid, or both are toxic β-cells, suggesting that formation may contribute pathology There relatively few reported inhibitors by IAPP. Here we show tea-derived flavanol, (-)-epigallocatechin 3-gallate...

The development of cancer nanotherapeutics has attracted great interest in the recent decade. Cancer have overcome several limitations conventional therapies, such as nonspecific biodistribution, poor water solubility, and limited bioavailability. Nanoparticles with tuned size surface characteristics are key components nanotherapeutics, designed to passively or actively deliver anti-cancer drugs tumor cells. We provide an overview nanoparticle-based drug delivery methods therapies based on...

Combining immune checkpoint blockade (ICB) therapy with photodynamic (PDT) holds great potential in treating immunologically "cold" tumors, but photo-generated reactive oxygen species (ROS) can inevitably damage co-administered ICB antibodies, hence hampering the therapeutic outcome. Here we create a ROS-responsive hydrogel to realize sustained co-delivery of photosensitizers and antibodies. During PDT, skeleton poly(deca-4,6-diynedioic acid) (PDDA) protects antibodies by scavenging harmful...

Aggregation and amyloid formation play critical roles in a range of human diseases including type 2 diabetes. Islet polypeptide (IAPP or amylin) is responsible for In vitro, IAPP one the most amyloidogenic naturally occurring sequences known. Thus development effective inhibitors extremely challenging. We demonstrate that single-point mutant, I26P, converts from highly into potent inhibitor formation. The mutant exerts pronounced effects on fibril by wild-type even at an equimolar ratio....

Islet amyloidosis by IAPP contributes to pancreatic β-cell death in diabetes, but the nature of toxic species remains elusive. Using concurrent time-resolved biophysical and biological measurements, we define produced during amyloid formation link their properties induction rat INS-1 murine islet toxicity. These globally flexible, low order oligomers upregulate pro-inflammatory markers induce reactive oxygen species. They do not bind 1-anilnonaphthalene-8-sulphonic acid lack extensive...

Abstract Live-cell Raman imaging based on bioorthogonal probes with distinct signals in the cellular Raman-silent region (1800–2800 cm −1 ) has attracted great interest recent years. We report here a class of water-soluble and biocompatible polydiacetylenes intrinsic ultrastrong alkyne that locate this for organelle-targeting live-cell imaging. Using host-guest topochemical polymerization strategy, we have synthesized functionalizable master polydiacetylene, namely poly(deca-4,6-diynedioic...

Abstract A dual‐functional photosensitizer that demonstrates exceptional photodynamic therapy (PDT) efficacy while simultaneously self‐monitoring the therapeutic response in real time is reported here. Possessing an ultrahigh 1 O 2 quantum yield of 98.6% water, TPCI can efficiently induce cell death a series carcinoma cells (IC 50 values less than 300 × 10 −9 m ) upon irradiation with extremely low fluence (460 nm, 4 mW cm −2 for min). In addition, self‐monitor time. It weakly fluorescent...

This review summarized most recent advances of designing strategies polydiacetylene-based smart biomaterials with unique colorimetric and mechanical properties, as well their applications in biosensing, drug delivery, tissue engineering.

Simple, rapid, and sensitive assays of DNA sequence hold great importance in genetic analysis, clinical diagnosis, molecular biology research. Most current methods for detection, based on the complementary base pairing, require hybridization with intricately modified single-stranded (ssDNA) probes or analytes. Herein, we have developed a powerful molecule aggregation-induced emission (AIE) characteristic, namely, TPBT, which can specifically recognize double-stranded (dsDNA) by emitting out...

Efficient therapeutic strategies that concurrently target both Aβ aggregation and oxidative stress in the Alzheimer's disease (AD) microenvironment emerge as a cutting-edge tool to combat intricate pathogenesis of AD. Here, multivalent nanobody conjugate with rigid, reactive oxygen species (ROS) scavenging scaffold is developed achieve simultaneous amyloidogenesis mitigation, ROS elimination, plaque clearance. Grafting segment (33-GLMVGGVVIA-42) into third complementary-determining region...

Diabetic wounds are usually entangled in a disorganized and self-perpetuating microenvironment accompanied by prolonged delay tissue repair. Sustained coordinated regulation regeneration key to the healing process of diabetic wounds, yet they continue pose formidable challenge. Here we report rational double-layered dressing design based on chitosan degradable conjugated polymer polydiacetylene, poly(deca-4,6-diynedioic acid) (PDDA), that can meet this intricate requirement. With an...

The rapid development of digital society and artificial intelligence has triggered explosive demands for specialty plastics, especially conjugated polymers that are instrumental flexible electronics smart devices. recycling degradation postconsumer have become more important than ever to reduce the pressure environment. Here we report discovery an environmentally self-degradable polymer poly(deca-4,6-diynedioic acid), or PDDA. PDDA is stable in dark without oxygen when used as a functional...

The highly immunosuppressive microenvironment after surgery has a crucial impact on the recurrence and metastasis in breast cancer patients. Programmable delivery of immunotherapy-involving combinations through single drug system is promising, yet greatly challenging, to reverse postoperative immunosuppression. Here, an injectable hierarchical gel matrix, composed dual lipid (DLG) layers with different soybean phosphatidylcholine/glycerol dioleate mass ratios, was developed achieve...

Abstract Two‐photon photodynamic therapy (2P‐PDT) that employs photosensitizers (PSs) with 2P absorption is particularly intriguing in cancer treatment, excitation enables precise spatial localization and deep tissue penetration. Here, a donor‐ π ‐acceptor PS (named TPBPy) near infrared (NIR) aggregation‐induced emission (AIE) designed synthesized for imaging‐guided 2P‐PDT. The maximal photoluminescence (PL) peak of TPBPy as high 720 nm when it encapsulated liposomes. Upon irradiation by...

Accurate diagnosis and timely therapeutic intervention of inflammatory bowel disease (IBD) is essential in preventing the progression disease, although it still represents an insurmountable challenge. Here we report design bacterial-flagella-inspired polydiiododiacetylene (PIDA) nanofibers its performance targeted computed tomography (CT) imaging on-demand IBD. With a morphology mimicking bacterial flagella, PIDA attach on mucus layer gastrointestinal (GI) tract after oral administration,...

Amyloid formation has been implicated in more than 20 different human diseases, including Alzheimer's disease, Parkinson's and type 2 diabetes. The development of inhibitors amyloid is a topic considerable interest, both because their potential therapeutic applications they are useful mechanistic probes. Recent studies have highlighted the use rifampicin as an inhibitor by variety polypeptides; however, there conflicting reports on its ability to inhibit islet polypeptide (IAPP). IAPP cause...

Islet amyloid polypeptide (IAPP) is a 37-residue hormone that responsible for islet formation in type II diabetes. Human IAPP extremely amyloidogenic, while rat and mouse do not form vitro or vivo. Rat have identical primary sequences, but differ from the human at six positions, five of which are localized between residues 20 29. The ability to inhibit by was tested, peptide found be an effective inhibitor. Thioflavin-T fluorescence-monitored kinetic experiments, transmission electron...

Photodynamic theranostics that allows for concurrent photodynamic therapy (PDT) and precise therapeutic response report has emerged as an intriguing direction in the development of precision medicine. An ultra-efficient platform was developed here based on combining potentiating a theranostic photosensitizer, TPCI, with other therapies synergistic anticancer effect synchronous self-reporting response. In this study, TPCI chemotherapy agent paclitaxel (PTX) were co-encapsulated liposomes,...

Abstract Ferroptosis is a recently-defined tumor suppression mechanism, but the sensitivity of many tumorigenic cells to ferroptosis limited by their deficient expression acyl-CoA synthetase long-chain family member 4 (ACSL4). Here, we report discovery photosensitizer, namely TPCI, which can evoke ACSL4-independent cancer in photodynamic therapy. Through co-localization with 12-lipoxygenase (ALOX12) multiple subcellular organelles, TPCI activates ALOX12 generate lipid reactive oxygen species...

Radiotherapy is a mainstay treatment used in clinics for locoregional therapy, although it still represents great challenge to improve the sensitivity and accuracy of radiotherapy tumors. Here, we report conjugated polymer, polydiiododiacetylene (PIDA), with an iodine content 84 wt %, as highly effective computed tomography (CT) contrast agent tumor microenvironment-responsive radiosensitizer. PIDA exhibited several key properties that contribute improvement precision radiotherapy. The...

Abstract α‐Synuclein (α‐Syn) amyloidogenesis is considered a promising diagnostic marker and therapeutic target for Parkinson's disease (PD). Simultaneously visualizing mitigating α‐Syn are essential future PD theranostics, yet they continue to pose an insurmountable challenge. This study have herein developed nanobody‐decorated polydiacetylene approach straightforward solution. Grafting α‐Syn61‐95 segment into the third complementary determining region of parent nanobody generates...

Cationic photosensitizers (PSs) offer many intriguing advantages, in addition to generating heat or reactive oxygen species for cancer phototherapy. However, the preparation of cationic PSs with enhanced near-infrared (NIR) absorption remains a significant challenge. In this work, we have synthesized PS TPBBT, which incorporates strong electron-withdrawing unit, benzobisthiadiazole, and four terminal pyridinium groups. It self-assembles into mixed H/J aggregated state maximal peak at 620 nm...