A5026132509- Lymphatic System and Diseases
- Sympathectomy and Hyperhidrosis Treatments
- Aerodynamics and Fluid Dynamics Research
- Cellular Mechanics and Interactions
- Effects of Vibration on Health
- Planarian Biology and Electrostimulation
- Diagnosis and Treatment of Venous Diseases
- Lymphatic Disorders and Treatments
- Myofascial pain diagnosis and treatment
- Fluid Dynamics and Turbulent Flows
- Skin and Cellular Biology Research
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Tendon Structure and Treatment
- Ion channel regulation and function
- Thermoregulation and physiological responses
- Myasthenia Gravis and Thymoma
- Neonatal Respiratory Health Research
- Ion Transport and Channel Regulation
- Cerebrospinal fluid and hydrocephalus
- Wnt/β-catenin signaling in development and cancer
- Spaceflight effects on biology
- Cystic Fibrosis Research Advances
- Blood properties and coagulation
- Ear and Head Tumors
Georgia Institute of Technology
2015-2025
Texas A&M Health Science Center
2010-2014
Temple College
2012
Lymphedema is a common debilitating condition with very limited treatment options, and leukotriene B 4 may be key pathogenic molecule therapeutic target.
Numerous studies on metabolic syndrome (MetSyn), a cluster of abnormalities, have demonstrated its profound impact cardiovascular and blood microvascular health; however, the effects MetSyn lymphatic function are not well understood. We hypothesized that would modulate muscle activity alter muscularized similar to impairment vessel associated with MetSyn, particularly given direct proximity lymphatics chronically inflamed adipose depots. To test this hypothesis, rats were placed...
Given the known mechanosensitivity of lymphatic vasculature, we sought to investigate effects dynamic wall shear stress (WSS) on collecting vessels while controlling for transmural pressure. Using a previously developed ex vivo perfusion system (ELPS) capable independently both transaxial pressure gradient and average an isolated vessel, imposed multitude flow conditions rat thoracic ducts, measuring diameter changes. By gradually increasing through determined WSS at which vessel first shows...
Muscular lymphatics use both phasic and tonic contractions to transport lymph for conducting their vital functions. The molecular mechanisms regulating lymphatic muscle are not well understood. Based on the well-established finding that phosphorylation of myosin light chain 20 (MLC(20)) plays an essential role in blood vessel smooth contraction, we investigated if phosphorylated MLC(20) (pMLC(20)) would modulate and/or muscle. effects ML-7, a MLC kinase inhibitor (1-10 microM), were tested...
The lymphatic system plays complex, often contradictory, roles in many cancers, including melanoma; these include contributions to tumor cell metastasis and immunosuppression the microenvironment as well generation of antitumor immunity. Advancing our understanding vessel involvement regulating growth immune response may provide new therapeutic targets or treatment plans enhance efficacy existing therapies. We utilized a syngeneic murine melanoma model which we surgically disrupted network...
Abstract It has been suggested that many forms of secondary lymphedema in humans are driven by a progressive loss lymphatic pump function after an initial risk-inducing event. However, the link between failure and disease progression remained elusive due to experimental challenges clinical setting lack adequate animal models. Using novel surgical model injury, we track adaptation functional decline network response surgery. This mimics histological hallmarks typical mouse tail while leaving...
The aim of this study was to elucidate the molecular signaling mechanisms by which substance P (SP) modulates lymphatic muscle contraction and determine whether SP stimulates both contractile as well inflammatory pathways in lymphatics.A rat mesenteric cell culture model (RMLMCs) known specific pharmacological inhibitors were utilized delineate SP-mediated lymphatics.We detected expression neurokinin receptor 1 (NK1R) 3 (NK3R) RMLMCs. stimulation increased phosphorylation myosin light chain...
Non‐Technical Summary The contractile activities of lymphatic muscle cells, which are the key for function, vary between beds. We show that pressure‐dependent changes and myosin light chain kinase (MLCK) inhibition differentially affect 20 (MLC ) phosphorylation in muscle, thereby controlling behaviour thoracic duct cervical lymphatics. Our findings suggest pressure‐induced function act through MLC di‐phosphorylation to modulate tonic contractions, whereas MLCK inhibitor ML‐7 affects both...
Mechanical loading conditions are likely to play a key role in passive and active (contractile) behaviour of lymphatic vessels. The development microstructurally motivated model tissue is necessary for quantification mechanically mediated maladaptive remodelling the vasculature. Towards this end, we performed cylindrical biaxial testing Sprague-Dawley rat thoracic ducts (n = 6) constitutive modelling characterize their mechanical behaviour. Spontaneous contraction was quantified at...
Abstract Lymphedema, a disfiguring condition characterized by an asymmetrical swelling of the limbs, is suspected to be caused dysfunctions in lymphatic system. A possible source dysfunction reduced mechanosensitivity lymphangions, spontaneously contracting units In this study, entrainment lymphangions oscillatory wall shear stress (OWSS) rat thoracic ducts relation their sensitivity. The critical above which show substantial inhibition contraction was found significantly negatively...
The contractile activity of muscle cells lining the walls collecting lymphatics is responsible for generating and regulating flow within lymphatic system. Activation PKC signaling contributes to regulation smooth contraction by enhancing sensitivity apparatus Ca 2+ . It currently unknown whether contraction. We hypothesized that activation would increase myofilament To test this hypothesis, we determined effects with phorbol esters [PMA or dibutyrate (PDBu)] on behavior α-toxin-permeabilized...
Previous studies have shown the ability of many lymphatic vessels to contract phasically pump lymph. Every lymphangion can act like a heart with pacemaker sites that initiate phasic contractions. The contractile wave propagates along vessel synchronize contraction. However, determining location within these has proven be very difficult. A high speed video microscopy system an automated algorithm detect and calculate propagation velocity, speed, duration, frequency contractions is presented...
We describe the development of an optical flow visualization method for resolving velocity vector field in lymphatic vessels vitro. The aim is to develop experimental protocol accurately estimating parameters, such as rate and shear stresses, with high spatial temporal resolution. Previous studies situ have relied on lymphocytes tracers, but their low density resulted a reduced resolution whereas assumption that was fully developed order determine parameters interest may not be valid,...
The intrinsic contraction of collecting lymphatic vessels serves as a pumping system to propel lymph against hydrostatic pressure gradients it returns interstitial fluid the venous circulation. In present study, we proposed and validated that maximum opposing outflow along chain lymphangions at which flow can be achieved increases with length chain. Using minimally invasive near-infrared imaging measure effective various locations in rat tail, demonstrated tail. Computational simulations...
Abstract The lymphatic system has been proposed to play a crucial role in preventing the development and progression of osteoarthritis (OA). As OA develops progresses, inflammatory cytokines degradation by-products joint tissues build up synovial fluid (SF) providing feedback exacerbate disease. plays critical resolving inflammation maintaining overall homeostasis; however, there is some evidence that lymphatics can become dysfunctional during OA. We hypothesized functional mechanics vessels...
We present the first in vivo evidence that lymphatic contraction can entrain with an external oscillatory mechanical stimulus. Lymphatic injury alter collecting contractility, but not much is known about how its mechanosensitivity to pressure affected, which crucial given current application methods for treating lymphoedema. show waves (OPW), akin intermittent pneumatic compression (IPC) therapy, optimally contractility and modulate function depending on frequency propagation speed of OPW....
Significance: Changes in interstitial fluid clearance are implicated many diseases. Using near-infrared (NIR) imaging with properly sized tracers could enhance our understanding of how venous and lymphatic drainage involved disease progression or drug delivery strategies. Aim: We investigated multichromatic NIR multiple to assess vivo microvascular kinetics pathways different tissue spaces. Approach: used a chemically inert IR Dye 800CW (D800) target capillaries purified conjugate dye 680RD...
C-reactive protein (CRP) is the classic acute phase reactant with serum levels rapidly increasing up to a thousand-fold during inflammatory responses. Sequential CRP in 2–10 μg/ml range have been suggested as prognostic indication of subsequent cardiovascular event. Lymphatics play an important role cardiovasculature providing fluid transport proteins, products tissue disintegration and infectious agents, thereby major edema formation immune response. We proposed that correlation existed...
Calcium plays essential roles in the contraction of striated and smooth muscle types. Changes cytoplasmic concentration Ca 2+ depend general on calcium channels pumps at endoplasmic/sarcoplasmic reticulum membrane. As we have shown previously, lymphatic has a unique combination contractile regulatory proteins (Muthuchamy et al., 2003). However, very little is known about composition ‐channels pumping structures intracellular stores muscle. Our immunohistochemistry data with confocal...