ABSTRACT Current strategies for bone regeneration after traumatic injury often fail to provide adequate healing and integration. Here, we combined the poly (ethylene glycol) diacrylate (PEGDA) hydrogel with allogeneic “carrier” cells transduced an adenovirus expressing BMP2. The system is unique in that biomaterial encapsulates cells, shielding them thus suppressing destructive inflammatory processes. Using this system, complete of a 5 mm‐long femur defect rat model occurs under 3 weeks,...

To evaluate the feasibility of short-term 3-D tissue culture in maintaining structure and function equine synovial explants demonstrate that day 4 explant characteristics were not significantly different from 0. We hypothesized a 4-day period would disrupt health compared to baseline measurements. Synovial (n = 24/horse) healthy carpal joints 5 horses cultured 12-well plates using tissue-stabilizing agar rings. Explants evaluated triplicate or quadruplicate at days 0, 2, for metabolism,...

Abstract Objective —To compare measurements of myeloperoxidase (MPO) in plasma, laminar tissues, and skin obtained from control horses given black walnut heartwood extract (BWHE). Animals —22 healthy 5- to 15-year-old horses. Procedures —Horses were randomly assigned 4 groups as follows: a group water (n = 5) 3 experimental BWHE (17) via nasogastric intubation. Experimental consisted 5, 6, 6 that received euthanatized at 1.5, 3, 12 hours after intubation, respectively. Control Plasma samples...

Mesenchymal stem cells (MSCs) produced for clinical purposes rely on culture media containing fetal bovine serum (FBS) which is xenogeneic and has the potential to significantly alter MSC phenotype, rendering these immunogenic. As a result of bovine-derived exogenous proteins expressed cell surface, MSCs may be recognized by host immune system as non-self rejected. Platelet lysate (PL) obviate some concerns shows promising results in human medicine possible alternative FBS. Our goal was...

The goal of this study was to establish an SPIO-based cell-tracking method in ovine model tendonitis and determine if may be useful for further cellular therapies vivo. Functional assays were performed on labeled unlabeled cells ensure that no significant changes induced by intracellular SPIOs. Following biosafety validation, tendon lesions mechanically (n = 4) or chemically four sheep scanned ex vivo at 7 14 days the presence distribution intralesional cells. Ovine MSCs with 50 µg SPIOs/mL...

Abstract OBJECTIVE To investigate risk factors for the development of pasture- and endocrinopathy-associated laminitis (PEAL) in horses ponies North America. DESIGN Case-control study. ANIMALS 199 with incident cases PEAL 351 from 2 control populations (healthy [n = 198] lameness not caused by [153]) that were evaluated America between January 2012 December 2015 veterinarian members American Association Equine Practitioners. PROCEDURES Practitioners contacted to participate study,...

Six normal, healthy horses age 3-10 years underwent left and right thoracoscopic examination using a rigid telescope. A minimum of 30 days was allowed between procedures. Horses were restrained in stocks sedated with continuous detomidine infusion. After surgical preparation the hemithorax elected for surgery, administration local or regional anaesthesia surgery sites, thoracoscopy completed during two 15 min pneumothorax periods. During procedures, thoracic structures viewed 57 cm, 10 mm...

Equine laminitis is a crippling condition associated with variety of systemic diseases. Although it apparent that the prodromal stages involve microvascular dysfunction, little known regarding physiology this vasculature. The aim present study was to determine relative responses equine laminar arteries and veins vasoconstrictor agonists phenylephrine (1 nM-10 microM), 5-HT PGF2alpha nM-100 endothelin-1 pM-1 microM). We have determined were more sensitive, respect concentration agonist...

Abstract Objective —To evaluate the incidence of colic and risk factors for in equids hospitalized ocular disease. Design —Retrospective observational study. Animals —337 (317 horses, 19 ponies, 1 donkey) Procedures —Medical records > 24 hours treatment disease between January 1997 December 2008 were reviewed. Information from only first hospitalization was used that on more than occasion. gathered included signalment, type lesion administered, any signs recorded during as well severity,...

Equine laminitis purportedly involves haemodynamic dysfunction at the level of laminar vasculature. However, to date, no studies have been performed characterising function arteries and veins during prodromal stages equine laminitis.That are associated with contractile vasculature.To assess phenylephrine (PE) 5-hydroxytryptamine (5-HT).Horses were administered black walnut heartwood extract (BWHE) or water (control horses) via nasogastric intubation. After euthanasia, vessels (100-800 microm...

Objectives: To determine (1) the short- (to hospital discharge) and long- (>6 months) term survival, (2) factors associated with short-term (3) perioperative course for horses resection anastomosis of descending colon. Study Design: Multicentered case series. Animals: Horses (n=43) that had colon anastomosis. Methods: Medical records (January 1995–June 2009) 7 equine referral hospitals were reviewed recovered from anesthesia. Retrieved data included history, results clinical...

Six healthy, awake, and pharmacologically restrained mature horses were studied in order to define the changes cardiopulmonary function during after exploratory thoracoscopy determine presence of postoperative complications occurring 48 hours thoracoscopy. In a randomised 3 x latin square design with 2 replications, 18 procedures performed: 6 right (RTH) left thoracoscopies (LTH) sham (STH). Prior each procedure physical examination bronchoalveolar lavage fluid analysis performed. During...

To evaluate the safety and efficacy of thoracoscopically guided pulmonary wedge resection in horses.10 horses (5 control 5 affected with recurrent airway obstruction [ie, heaves]).Each horse underwent a resection. Before, during, after surgery, heart rate, respiratory arterial blood gases, systemic pressures were measured. Physical examination, CBC, thoracic radiography ultrasonography performed 24 hours before 2 48 surgery. Pulmonary specimens assessed by histologic examination. A second...

Summary Reasons for performing study: Laminitis is a serious complication of horses suffering from sepsis/endotoxaemia‐related events. in and organ injury human sepsis are both reported to involve inflammatory the laminae/organs including early activation endothelium leucocytes leading emigration neutrophils into tissue interstitium. In black walnut extract (BWE) model, systemic events coincide with marked increase laminar mRNA concentrations genes proinflammatory cytokines (i.e. IL‐1β,...

Autologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating development of alternative therapeutics. Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together induce rapid formation. However, methods make these treatments available clinical applications presently lacking. In this study we used mesenchymal stem cells (MSCs) due their ease harvest, replication potential,...

Summary This reports describes the surgical management of a horse evaluated for recurrent colic. These frequent colic episodes were attributed by exclusion to an abnormally enlarged spleen (idiopathic splenomegaly). Splenectomy was elected and performed with laparoscopic assisted technique. The advantages observed using this approach clear view visceral aspect consequently accurate dissection hilus associated vasculature. Ultimately technique allowed surgeons reduce size laparotomy incision...

Abstract A 5‐year‐old Draft Horse gelding presented for evaluation of a large, fleshy, ulcerated third eyelid mass OD 3 weeks duration. Complete ophthalmic examination, ocular ultrasound and skull radiographs revealed large soft‐tissue involving the entire extending into ventral right orbit to level globe equator. No other abnormalities were noted on physical or examination. Surgical removal via exenteration was performed months after initial presentation. lacrimal adenocarcinoma diagnosed...

Platelet preparations containing growth factors, attachment and enzymes are appealing to enhance healing of injured tissues as an alternative xenogenic serum in cell culture media. Plateletpheresis is commonly used collect platelets human medicine but has not been validated horses.Plateletpheresis platelet concentrate was performed on six female, mixed breed, chemically restrained horses using commercially available apheresis equipment. Before immediately after plateletpheresis, we physical...

Abstract Objective —To describe a single-portal cranial arthroscopic approach to the stifle joint in horses and determine clinical outcome with femorotibial disease which this was used. Design —Retrospective study. Animals —23 adult horses. Procedure —Medical records were reviewed obtain information on Results —Twenty-nine joints of 23 examined arthroscopically, using described approach. Subchondral bone cysts treated 19 medial femoral condyles 12 Unilateral cruciate ligament desmitis (4...