A5049402685- Reproductive Physiology in Livestock
- Parasitic Infections and Diagnostics
- Gut microbiota and health
- Ruminant Nutrition and Digestive Physiology
- Milk Quality and Mastitis in Dairy Cows
- Infant Nutrition and Health
- Mesenchymal stem cell research
- Amoebic Infections and Treatments
- Animal Virus Infections Studies
- Animal Genetics and Reproduction
- Probiotics and Fermented Foods
- Mycotoxins in Agriculture and Food
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Genetic and phenotypic traits in livestock
- Virus-based gene therapy research
- Selenium in Biological Systems
- Effects of Environmental Stressors on Livestock
- Extracellular vesicles in disease
- Liver Disease Diagnosis and Treatment
- Tissue Engineering and Regenerative Medicine
- Coccidia and coccidiosis research
- Protein Hydrolysis and Bioactive Peptides
- Animal Nutrition and Health
- Sesquiterpenes and Asteraceae Studies
Sichuan Agricultural University
2016-2025
Peking University People's Hospital
2025
Peking University
2025
Maternal and Child Health Hospital of Sichuan Province
2025
Fudan University
2023
Michigan State University
2015
University of Missouri
2006
Placenta is a kind of traditional Chinese medicine, known as "Ziheche", which has the function tonifying qi and blood, nourishing liver kidney. extract (PE) been used for delaying organismal aging treating various diseases. Cow placenta rich natural resource with large mass. Its composition similar to that human placenta, but it not effectively utilized. However, little about effect CPE on mice.
Background/Objectives: The transition from a non-lactating to lactating state is critical period for lipid metabolism in dairy cows. Danggui Buxue Tang (DBT), stimulating energy metabolism, ameliorates diseases related disorders and expected be an effective supplement alleviating excessive mobilisation periparturient This study aimed investigate the effects of supplemental DBT on serum biochemical indices, faecal microbial communities, plasma metabolites Methods: Thirty cows were randomly...
Based on the experimentally determined atomic coordinates for RNA helices and self-avoiding walks of P (phosphate) C4 (carbon) atoms in diamond lattice polynucleotide loop conformations, we derive a set conformational entropy parameters pseudoknots. parameters, develop folding thermodynamics model that enables us to compute sequence-specific pseudoknot free energy landscape thermodynamics. The is validated through extensive experimental tests both native structures predicts strong...
Abstract Genetic engineering in livestock was greatly enhanced by the emergence of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9), which can be programmed with a single-guide RNA (sgRNA) to generate site-specific DNA breaks. However, uncertainties caused wide variations sgRNA activity impede utility this system generating genetically modified pigs. Here, we described single blastocyst genotyping provide simple and rapid solution evaluate compare...
The metabolic responses of cows undergo substantial changes during the transition from late pregnancy to early lactation. However, molecular mechanisms associated with these in physiological metabolism have not been clearly elucidated. objective this study was investigate perspective plasma metabolites. Plasma samples collected 24 multiparous dairy on approximately d 21 prepartum and immediately postpartum were analyzed using ultra-high-performance liquid chromatography/time-of-flight mass...
Metabolic maladaptation in dairy cows after calving can lead to long-term elevation of ketones, such as β-hydroxybutyrate (BHB), representing the condition known hyperketonemia, which greatly influences health and production performance during lactation period. Although gut microbiota is alter with association microbial metabolites development hyperketonemia remains unknown. In this study, we performed a multi-omics analysis investigate associations between fecal community, fecal/plasma...
Viroporins are a family of low-molecular-weight hydrophobic transmembrane proteins that encoded by various animal viruses. form pores in host cells via oligomerization, thereby destroying cellular homeostasis and inducing cytopathy for virus replication virion release. Among the Picornaviridae viruses, 2B protein enteroviruses is well understood, whereas viroporin activity foot-and-mouth disease (FMDV) has not yet been described. An analysis FMDV domains computer-aided programs conducted...
Increasing evidences have demonstrated that Ageratina adenophora (A. adenophora) can cause hepatotoxicity of animals. Liver is an important site in immune regulation and inflammatory responses. However, the information about induced by A. relation to inflammation still finite. To investigate underlying mechanism, we conducted animal experiments with different dosage adenophora. Mice were randomly divided into 4 groups administrated 0%, 10%, 20% 30% levels pallet diet control, group A, B C,...
Abstract Virus entry is an attractive target for therapeutic intervention. Here, using a combination of electron microscopy, immunofluorescence assay, siRNA interference, specific pharmacological inhibitors and dominant negative mutation, we demonstrated that the foot-and-mouth disease virus (FMDV) triggered substantial amount plasma membrane ruffling. We also found internalization FMDV induced robust increase in fluid-phase uptake virions internalized within macropinosomes colocalized with...
Enterocytozoon bieneusi, the most commonly identified microsporidian species in humans, is also livestock, birds, rodents, reptiles, companion animals, even wastewater. However, there no information available on occurrence of E. bieneusi pet chipmunks. The aim present study was to determine genotypes, molecular characterization chipmunks, and assess zoonotic potential. A total 279 fecal specimens were collected from chipmunks seven shops one breeding facility Sichuan province, China....
Bovine mastitis is a common disease occurring in dairy farms and can be caused by more than 150 species of pathogenic bacteria. One the most causative organisms Streptococcus agalactiae , which also potentially harmful to humans aquatic animals. At present, research on S . China mostly concentrated northern region, with limited southeastern southwestern regions. In this study, total 313 clinical samples from large-scale five regions Sichuan were collected for isolation The epidemiological...
With the improper use of antibiotics, an increasing number multidrug-resistant bacteria have been reported worldwide, posing challenges for disease treatment. Klebsiella pneumoniae is important zoonotic pathogen that colonises respiratory tract. Endolysin therapy has emerged with development phages. In this study, a lytic phage vB_KpnS_MK54 was isolated from drinking water forest musk deer (FMD) farm in Sichuan Province. It first obtained FMD. The primary biological characteristics were...
In recent years, nonalcoholic fatty liver disease (NAFLD) has become the most common in world. As an important model animal, characteristics of gut microbiota alteration mice with NAFLD have been studied but changes metabolite abundance and how affects these intestinal metabolites remain unclear. this experiment, a mouse for was established by high-fat diet. The use 16S rDNA technology showed that while there were no significant alpha diversity cecum mice, beta changed significantly....
Dairy cows with ketosis have high circulating beta-hydroxybutyric acid (BHBA) concentrations alongside which inflammation is concomitantly developed. Tryptophan (Trp) an essential amino that participates in the regulation of inflammatory response. However, association between Trp metabolism and dairy remains unclear. Therefore, blood samples from healthy (n = 10) ketotic primiparous were collected at calving date day diagnosis (7 days milk DIM)). Serum levels non-esterified fatty acids...
Abstract Background The burden of IBD in the elderly is increasing globally. However, mechanisms underlying age-related susceptibility remain unclear. Aging leads to a progressive functional decline immune system, rendering increasingly susceptible inflammatory and autoimmune disease. As fundamental mRNA modification, N6-methyladenosine (m6A) participates various pathological processes. role METTL3-mediated m6A RNA modification remains Herein, we sought determine immunosenescence...
Postpartum dairy cows often face significant challenges due to metabolic disorders. Danggui Buxue Tang (DBT), a botanical drug composed of Astragali radix and Angelica sinensis in 5:1 ratio, has been recognized for its potential alleviate Its regulatory mechanisms on livestock health have remained unexplored. This study integrated the analyses serum pharmacochemistry, network pharmacology, metabolomics, fecal microbiota investigate effects DBT adaptation postpartum cows. Following oral...
The ketogenic diet (KD) induces prolonged hyperketonemia, characterized by elevated circulating level of β-hydroxybutyrate. However, the KD can negatively affect host metabolic health altering gut microbial community. Despite this, regulatory effect microbiota on hepatic ketogenesis and triacylglycerol (TAG) accumulation during a remains poorly understood. Here, we hypothesized that commensal bacterium regulates lipid metabolism in association with KD-induced hyperketonemia. disrupts...
Giardia duodenalis and Cryptosporidium spp. are common human animal pathogens. They have increasingly been reported in dairy calves recent years; however, multilocus genotyping information for G. infecting pre-weaned southwestern China is limited. In the present study, prevalence of central Sichuan province was determined pathogens were analyzed molecularly. Of 278 fecal samples from calves, 26 (9.4%) positive 40 (14.4%) bovis ( n = 28), ryanae 5) parvum 7) detected. All seven C. isolates...