A5048280974- MicroRNA in disease regulation
- RNA Research and Splicing
- RNA modifications and cancer
- Pregnancy and preeclampsia studies
- Neurogenetic and Muscular Disorders Research
- HIV Research and Treatment
- RNA regulation and disease
- Amyotrophic Lateral Sclerosis Research
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Genetics and Neurodevelopmental Disorders
- Erythrocyte Function and Pathophysiology
- Aquaculture disease management and microbiota
- Prenatal Screening and Diagnostics
- Genomic variations and chromosomal abnormalities
- Immune Cell Function and Interaction
- Nuclear Receptors and Signaling
- Synthetic Organic Chemistry Methods
- Phagocytosis and Immune Regulation
- Tryptophan and brain disorders
- Genomics and Rare Diseases
- Identity, Memory, and Therapy
- Genetic Neurodegenerative Diseases
- Stress Responses and Cortisol
- Hair Growth and Disorders
Tel Aviv University
2013-2023
Mislocalization of the predominantly nuclear RNA/DNA binding protein, TDP-43, occurs in motor neurons ~95% amyotrophic lateral sclerosis (ALS) patients, but contribution axonal TDP-43 to this neurodegenerative disease is unclear. Here, we show accumulation intra-muscular nerves from ALS patients and axons human iPSC-derived patient, as well neuromuscular junctions (NMJs) a mislocalization mouse model. In axons, hyper-phosphorylated promotes G3BP1-positive ribonucleoprotein (RNP) condensate...
MicroRNAs are short non-coding RNAs that play a central role in regulation of gene-expression by binding to target-gene mRNAs. Several studies have revealed alterations cellular-miRNA profiles following HIV-1 infection, mostly inhibiting viral-infection. These miRNA expression modifications may also serve block the innate inhibition mechanism. As result, it is expected during infection miRNAs target genes hinder or prevent progression different steps replication cycle. One major sets known...
Preeclampsia is one of the most dangerous pregnancy complications, and leading cause maternal perinatal mortality morbidity. Although clinical symptoms appear late, its origin early, hence detection feasible already at first trimester. In current study, we investigated abundance circulating small non-coding RNAs in plasma pregnant women their trimester, seeking transcripts that best separate preeclampsia samples from those healthy women. To this end, performed sequencing 75 control samples,...
Stress research has progressively become more integrative in nature, seeking to unfold crucial relations between the different phenotypic levels of stress manifestations. This study sought unravel stress-induced variations expression human microRNAs sampled peripheral blood mononuclear cells and further assess their relationship with neuronal psychological indices. We obtained samples from 49 healthy male participants before three hours after performing a social task, while undergoing...
Rapid responses to changes within subcellular compartments of highly polarized cells, such as neuron axons, depend on local translation and post-transcriptional regulation. The mechanism by which microRNAs (miRNAs) regulate this process is not fully understood. Here, using live cell imaging RNA sequencing analysis, we demonstrated how miRNAs can differentially control hundreds transcripts at the level. We that seed match length miRNA target-sequence regulates both mRNA stability protein...
HIV-1 is the causative agent of AIDS (Autoimmune Deficiency Syndrome). infection results in systemic CD4+ T cell depletion, thereby impairing cell-mediated immunity. MicroRNAs are short (~22 nucleotides long), endogenous single-stranded RNA molecules that regulate gene expression by binding to 3' untranslated regions (3' UTR) mRNA transcripts. The relation between and human miRNA profile has been previously investigated, studies have shown virus can alter vice versa. Here, we broaden...
Background: Selective serotonin reuptake inhibitor (SSRI) antidepressant drugs are the first-line of treatment for major depressive disorder (MDD) but effective in <70% patients. Our earlier genome-wide studies indicated that two genes encoding cell adhesion proteins, close homolog L1 (CHL1) and integrin beta-3 (ITGB3), microRNAs, miR-151a-3p miR-221/222, implicated variable sensitivity response human lymphoblastoid lines (LCL) from unrelated individuals to SSRI drugs. Methods: The microRNAs...
Abstract The impact of microRNAs (miRNAs) known to regulate numerous biologic processes on complement-dependent cytotoxicity (CDC) was investigated in K562 cells. C5b-9 complex is the executioner CDC. Cells protect themselves from CDC by elimination, a process involving mitochondrial chaperone mortalin/GRP75. Potential miR-200 (b and c) miR-217 regulatory sites were identified mortalin mRNA. Overexpression miR-200b/c or lowered expression miRNA inhibitors for miR-200b, miR-200c, enhanced...
Abstract Alopecia–neurological defects–endocrinopathy ( ANE ) syndrome is a rare inherited hair disorder, which was shown to result from decreased expression of the RNA ‐binding motif protein 28 RBM ). In this study, we attempted delineate role in biology. First, sought obtain evidence for direct involvement growth. When downregulated human follicle HF organ cultures, observed catagen induction and growth arrest, indicating that necessary normal We also aimed at identifying molecular targets...
A recent E-Rare workshop reviewed the ethical aspects of whole exome and genome-sequencing studies (WES WGS, respectively) in rare diseases. Leveraging new genomic technologies, which output vast amounts known novel genetic variants, researchers are learning more about basis mechanisms involved In some cases, these findings translated into diagnostic tools for benefit disease patients. Among disclosed data, can assist treatment management, incidental await, bringing with them concerns clinicians,
Preeclampsia is a dangerous pregnancy complication. The source of preeclampsia unknown, though the placenta believed to have central role in its pathogenesis. An association between maternal infection and has been demonstrated, yet involvement placental microbiome etiology not determined. In this study, we examined whether associated with an imbalanced microorganism composition placenta.To end, developed novel method for identification bacteria/viruses based on sequencing small non-coding...
Abstract Mislocalization of the predominantly nuclear RNA/DNA binding protein, TDP-43, occurs in motor neurons ~95% ALS patients, but contribution axonal TDP-43 to this fatal neurodegenerative disease is unclear. Here, we find accumulation axons intra-muscular nerves from and neuromuscular junctions (NMJs) a mouse model with mislocalization. This leads formation G3BP1- TDP-43- positive RNA-granules neuron axons, inhibition local protein synthesis NMJs. Specifically, synaptic levels...
Splicing of transcripts is catalyzed by the spliceosome, a mega-complex consisting hundreds proteins and five snRNAs, which employs direct interactions. When U1 snRNA forms high-affinity binding, namely more than eight base pairs, with 5'SS, result usually suppressing effect on splicing activity. This likely occurs due to inefficient unwinding U1/5'SS base-pairing or other regulatory obstructions. Here, we show in vitro patient-derived cell lines that pre-microRNAs can modulate reaction...
Rapid spatiotemporal responses within highly polarized cells, such as motor neurons (MN), depend on local translation and post-transcriptional regulation in distinct subcellular compartments axons. MicroRNAs (miRNAs) can regulate this process. Here, using live cell imaging RNA-sequencing, we demonstrated how miRNAs differentially control hundreds of transcripts at the resolution via variations seed length or repeats. Interestingly, transcriptome analysis neuron did not reveal any differences...