A5100780475- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Aquaculture disease management and microbiota
- Virology and Viral Diseases
- Viral Infections and Vectors
- Identification and Quantification in Food
- Herpesvirus Infections and Treatments
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Biosensors and Analytical Detection
- RNA and protein synthesis mechanisms
- Genetic diversity and population structure
- interferon and immune responses
- Marine Bivalve and Aquaculture Studies
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- Vector-Borne Animal Diseases
Guangxi Academy of Agricultural Science
2025
Rice Research Institute
2025
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2025
Shanghai Institute of Nutrition and Health
2024
Guangxi Veterinary Research Institute
2015-2024
University of Chinese Academy of Sciences
2024
Ministry of Agriculture and Rural Affairs
2022-2024
Chinese Academy of Sciences
2023
Hunan Agricultural University
2021
Nanning Normal University
2018
Low pathogenic avian influenza virus (LPAIV) usually causes mild disease or asymptomatic infection in poultry. However, some LPAIV strains can be transmitted to humans and cause severe infection. Genetic rearrangement recombination of even low may generate a novel with increased virulence, posing substantial risk public health. Southern China is regarded as the world "influenza epicenter", due rash outbreaks recent years. In this study, we conducted an epidemiological survey at different...
Abstract BACKGROUND The objective was to determine how molasses and Lactobacillus plantarum affect chemical composition, fermentation quality, aerobic stability, the microbial community of an ensiled mixture amaranth ( Amaranthus hypochondriaus , AF) rice straw. Treatments were control (C, no addition), L. (L; 2 × 10 5 cfu g −1 fresh weight), (M; 40 kg matter), their combination (LM). All treatments for 1, 3, 5, 7, 30 days. RESULTS additives improved quality with greater lactic acid (LA),...
Abstract An electrochemical immunoassay for the ultrasensitive detection of Newcastle disease virus (NDV) was developed using graphene and chitosan-conjugated Cu(I)/Cu(II) (Cu(I)/Cu(II)-Chi-Gra) signal amplification. Graphene (Gra) used both conjugation an anti-Newcastle monoclonal antibody (MAb/NDV) immobilization polyclonal antibodies (PAb/NDV). selected as electroactive probe, immobilized on a chitosan-graphene (Chi-Gra) hybrid material, detected by differential pulse voltammetry (DPV)...
Low temperature at the seedling stage adversely affects sorghum growth and development limits its geographical distribution. APETALA2/Ethylene-Responsive transcription factors (AP2/ERFs), one of largest factor families in plants, play essential roles growth, development, responses to abiotic stresses. However, AP2/ERF genes cold tolerance mechanisms underlying their effects remain largely unknown. Here, transcriptome sequencing (RNA-seq) was performed on leaves seedlings before after...
Influenza A viruses (IAVs) evade the immune system of host by several regulatory mechanisms. Their genomes consist eight single-stranded segments, including nonstructural proteins (NS), basic polymerase 1 (PB1), 2 (PB2), hemagglutinin (HA), acidic (PA), matrix (M), neuraminidase (NA), and nucleoprotein (NP). Some these are known to suppress responses. In this review, we discuss roles, functions underlying strategies adopted IAV escape targeting different in interferon (IFN) signaling...
Fowl adenovirus serotype 4 (FAdV-4) is highly pathogenic to broilers aged 3 5 weeks and has caused considerable economic loss in the poultry industry worldwide. FAdV-4 causative agent of hydropericardium-hepatitis syndrome (HHS) or hydropericardium (HPS). The virus targets mainly liver, HPS symptoms are observed infected chickens. This disease was first reported Pakistan but now spread worldwide, over time, various deletions FAdV genome mutations its major structural proteins have been...
Deoxynivalenol (DON), commonly known as vomitoxin, is a mycotoxin produced by fungi and frequently found contaminant in various cereal-based food worldwide. While the harmful effects of DON have been extensively studied different tissues, its specific impact on proliferation skeletal muscle cells remains unclear. In this study, we utilized murine C2C12 myoblasts model to explore influence their proliferation. Our observations indicated that exhibits dose-dependent toxicity, significantly...
Abstract Background Recent epidemiological investigation of different HA subtypes avian influenza viruses (AIVs) shows that the H3 subtype is most predominant among low pathogenic AIVs (LPAIVs), and seasonal variations in isolation are consistent with human viruses. Consequently, development a rapid, simple, sensitive detection method for required. The loop-mediated isothermal amplification (LAMP) assay cost-effective nucleic acid does not require any specialized equipment. Results A reverse...
We report a novel electrochemical immunosensor that can sensitively detect avian influenza virus H5 subtype (AIV H5) captured by graphene oxide-H5-polychonal antibodies-bovine serum albumin (GO-PAb-BSA) nanocomposite. The oxide (GO) carried H5-polychonal antibody (PAb) were used as signal amplification materials. Upon amplification, the showed 256-fold increase in detection sensitivity compared to without GO-PAb-BSA. designed PAb labeling GO strategy and procedure allow ultrasensitive...
Infections with low pathogenic avian influenza viruses (LPAIVs) can be mild or asymptomatic in poultry; however, humans, LPAIVs cause severe infections and death, as demonstrated by the H7N9 H10N8 human infection outbreaks 2013 China. In this study, we conducted an epidemiological survey of at live poultry markets (LPMs) Guangxi Province, Southern China, which is near several Southeast Asian countries. From January 2012 to December 2015, collected 3,813 swab samples from LPMs Guangxi. Viral...
Chicken parvovirus (ChPV), chicken infectious anaemia virus (CIAV) and fowl adenovirus serotype 4 (FAdV-4) are avian viruses that have emerged in recent years endangered the global poultry industry, causing great economic loss. In this study, a multiplex fluorescence-based loop-mediated isothermal amplification (mLAMP) assay for detecting ChPV, CIAV FAdV-4 was developed to simultaneously diagnose single mixed infections chickens. Three primer sets composite probes were designed according...
The H10 subtype avian influenza viruses (H10N4, H10N5 and H10N7) have been reported to cause disease in mammals, the first human case of H10N8 virus was 2013. Recently, (AIVs) followed more closely, but routine diagnostic tests are tedious, less sensitive time consuming, rapid molecular detection assays for AIVs not available. Based on conserved sequences within HA gene AIVs, specific primer sets were designed assay reaction conditions optimized. A reverse-transcription loop-mediated...
Interferon-inducible transmembrane protein 3 (IFITM3) is an antiviral factor that plays important role in the host innate immune response against viruses. Previous studies have shown IFITM3 upregulated various tissues and organs after avian reovirus (ARV) infection, which suggests may be involved ARV infection. In this study, chicken gene was cloned analyzed bioinformatically. Then, of infection further explored. The results showed molecular weight approximately 13 kDa. This found to...
A loop-mediated isothermal amplification (LAMP) assay was optimized for the rapid detection of Group I avian adenoviruses. set six primers designed from DNA sequences hexon genes adenovirus. The performed in a water bath 60 min at 63 C, and result visualized by adding fluorescence dye reagent or inspecting white sediment. results showed that LAMP could detect all 12 serotypes adenovirus nine Guangxi isolates. This I-specific 238 copies No cross-reactions were detected using with adenoviruses...
Duck circovirus (DuCV) infection in farmed ducks is associated with growth problems or retardation syndromes. Rapid identification of DuCV infected essential to control effectively. Therefore, this study aims develop an assay for be highly specific, sensitive, and simple without any specialized equipment. A set six specific primers was designed target the sequences Rep gene DuCV, loop-mediated isothermal amplification (LAMP) were developed reaction conditions optimized rapid detection DuCV....
Immunosuppressive viruses are frequently found as co-infections in the chicken industry, potentially causing serious economic losses. Because traditional molecular biology methods have limited detection ability, a rapid, high-throughput method for differential diagnosis of these is needed. The objective this study to develop GenomeLab Gene Expression Profiler Analyser-based multiplex PCR (GeXP-multiplex PCR) simultaneous eight immunosuppressive viruses. Using chimeric primers, such viruses,...
Loop-mediated isothermal amplification (LAMP) is a nucleic acid technique that can be used to amplify target genes at constant temperature, and it has several advantages, including convenience, specificity sensitivity. However, due the special interpretation methods of this technology for reaction results, all previously reported LAMP detection have been restricted identifying single target, which limits application technology. In study, we modified conventional include quencher-fluorophore...