A5101975070- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Fungal and yeast genetics research
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Polysaccharides and Plant Cell Walls
- Fungal Biology and Applications
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Advanced Cellulose Research Studies
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Catalysis for Biomass Conversion
- Microbial Metabolism and Applications
- Plant Gene Expression Analysis
- Nanoparticles: synthesis and applications
- Cassava research and cyanide
- Insect Pest Control Strategies
- Fermentation and Sensory Analysis
- Graphene and Nanomaterials Applications
- Studies on Chitinases and Chitosanases
- Nanomaterials for catalytic reactions
Guangxi University
2016-2025
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2015-2023
Google (United States)
2018
Tobacco Research Institute
2013
Guangxi Academy of Sciences
2010
Peking University
2006
Sainsbury Laboratory
1998-2003
John Innes Centre
1998-2003
Norwich Research Park
1998-2003
National University of Ireland
2003
Mutations in the seven clustered rpf genes cause downregulated synthesis of extracellular enzymes and reduced virulence Xanthomonas campestris pathovar ( Xcc ). The phenotype mutants one genes, rpfF , can be restored by a diffusible factor (DSF) produced all strains tested, apart from rpfB mutants. DSF accumulates early stationary phase (when is maximal), but levels decline subsequently. Addition to exponentially‐growing wild‐type bacteria does not precocious enzyme synthesis. are expressed...
The rpf gene cluster of Xanthomonas campestris pathovar ( Xcc ) is required for the pathogenesis this bacterium to plants. Several genes are involved in coordinate positive regulation production virulence factors mediated by small diffusible molecule DSF (for signal factor). RpfF directs synthesis DSF, and a two-component sensory transduction system comprising RpfC RpfG has been implicated perception transduction. In L medium, rpfF , rpfG, rpfC rpfGHC mutants grew as matrix-enclosed...
Xanthomonas campestris pathovar (Xcc) is the causative agent of crucifer black rot disease, which causes severe losses in agricultural yield world-wide. This bacterium a model organism for studying plant-bacteria interactions. We sequenced complete genome Xcc 8004 (5,148,708 bp), highly conserved relative to that ATCC 33913. Comparative genomics analysis indicated that, addition significant genomic-scale rearrangement cross replication axis between two IS1478 elements, loss and acquisition...
African strains of Xanthomonas oryzae pv. contain fewer TAL effectors than Asian strains, and their contribution to pathogenicity is unknown. Systematic mutagenesis tal genes was used decipher the each eight effector paralogs X. BAI3. A strain mutated in talC severely affected production disease symptoms. Analysis growth planta upon leaf-clip inoculation showed that mutant bacteria multiplied only at site apex leaf, suggesting a requirement for during colonization vascular tissues. Such...
From different natural reserves in the subtropical region of China, a total 245 aerobic bacterial strains were isolated on agar plates containing sugarcane bagasse pulp as sole carbon source. Of strains, 22 showed hydrolyzing zones carboxymethyl cellulose after Congo-red staining. Molecular identification that belonged to 10 genera, with Burkholderia genus exhibiting highest strain diversity and accounting for 36.36% all strains. Three isolates among higher cellulase (CMCase) activity,...
To clone and characterize genes encoding novel cellulases from metagenomes of buffalo rumens.A ruminal metagenomic library was constructed functionally screened for cellulase activities 61 independent clones expressing were isolated. Subcloning sequencing 13 positive endoglucanase MUCase identified 14 genes. Two carried two gene clusters that may be involved in the degradation polysaccharide nutrients. Thirteen recombinant partially characterized. They showed diverse optimal pH 4 to 7. Seven...
Starch is a very abundant and renewable carbohydrate an important feedstock for industrial applications. The conventional starch liquefaction saccharification processes are energy-intensive, complicated, not environmentally friendly. Raw starch-digesting glucoamylases capable of directly hydrolyzing raw to glucose at low temperatures, which significantly simplifies processing reduces the cost producing starch-based products.A novel glucoamylase PoGA15A with high enzymatic activity was...
Cellulose is the most abundant biomass on earth. The major players in cellulose degradation nature are cellulases produced by microorganisms. Aerobic filamentous fungi main sources of commercial cellulase. Trichoderma reesei has been explored extensively for cellulase production; however, its limitations low β-glucosidase activity and inefficiency degradation. aim this work was to isolate new fungal strains from subtropical tropical forests China, which produce high levels order facilitate...
Summary Cyclic di‐GMP is a second messenger with role in regulation of range cellular functions diverse bacteria including the virulence pathogens. Cellular levels cyclic are controlled through synthesis, catalysed by GGDEF protein domain, and degradation EAL or HD‐GYP domains. Here we report comprehensive study signalling bacterial disease which examine contribution all proteins GGDEF, domains to factor production phytopathogen Xanthomonas campestris pathovar ( Xcc ). Genes significant...
Alpha-amylase is a very important enzyme in the starch conversion process. Most of α-amylases are calcium-dependent and exhibit poor performance simultaneous saccharification fermentation process industrial bioethanol production that uses as feedstock. In this study, an extracellular amylolytic was purified from culture broth newly isolated Talaromyces pinophilus strain 1-95. The enzyme, with apparent molecular weight 58 kDa on SDS-PAGE, hydrolyzed maltopentaose, maltohexaose, maltoheptaose...
The filamentous fungus Penicillium oxalicum is a potential alternative to Trichoderma reesei for industrial production of complete cellulolytic enzyme system bio-refinery. Comparative omics approaches can support rational genetic engineering and/or breeding fungi with improved cellulase capacity. In this study, comparative genomic, transcriptomic and secretomic profiling P. HP7-1 its xylanase hyper-producing mutant EU2106 were employed screen novel regulators gene expression.The 30.62 Mb...
Xanthomonas campestris pathovar (Xcc) is the causal agent of black rot disease crucifers worldwide. The molecular genetic diversity and host specificity Xcc are poorly understood.We constructed a microarray based on complete genome sequence strain 8004 investigated by array-based comparative hybridization analyses 18 virulent strains. results demonstrate that core comprising 3,405 4,186 coding sequences (CDSs) spotted array conserved flexible gene pool with 730 CDSs absent/highly divergent...
Xanthomonas campestris pathovar causes black rot, a vascular disease on cruciferous plants, including Arabidopsis thaliana. The gene XC1553 from X. pv. strain 8004 encodes protein containing leucine-rich repeats (LRRs) and appears to be restricted strains of campestris. LRRs are found in number type III-secreted effectors plant animal pathogens. These prompted us investigate the role interaction between A. Translocation assays using hypersensitive-reaction-inducing domain AvrBs1 as reporter...
Cyclic di-GMP [(bis-(3'-5')-cyclic di-guanosine monophosphate)] is an almost ubiquitous second messenger in bacteria that implicated the regulation of a range functions include developmental transitions, aggregative behaviour, adhesion, biofilm formation and virulence. Comparatively little known about mechanism(s) by which cyclic exerts these various regulatory effects. PilZ has been identified as binding protein domain; proteins with this domain are involved specific cellular processes,...
Xanthomonas campestris pv ( Xcc ) is a plant pathogenic bacterium that controls the production of pathogenicity factors in part by cluster genes designated rpf (regulation factors). Sequence analysis one these rpfA revealed an open reading frame with amino acid sequence similarity to aconitases from other bacteria. Aconitase activity was lower cellular extracts ::Tn 5 mutant than those wild type. A zymogram aconitase after native gel electrophoresis showed presence two distinct ; major...