A5090631049- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- interferon and immune responses
- Respiratory viral infections research
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- Plant Virus Research Studies
- Viral Infections and Vectors
- Advanced biosensing and bioanalysis techniques
Guangxi Veterinary Research Institute
2022-2024
Ministry of Agriculture and Rural Affairs
2022-2024
Fowl adenovirus serotype 4 (FAdV-4) is highly pathogenic to broilers aged 3 5 weeks and has caused considerable economic loss in the poultry industry worldwide. FAdV-4 causative agent of hydropericardium-hepatitis syndrome (HHS) or hydropericardium (HPS). The virus targets mainly liver, HPS symptoms are observed infected chickens. This disease was first reported Pakistan but now spread worldwide, over time, various deletions FAdV genome mutations its major structural proteins have been...
Interferon-inducible transmembrane protein 3 (IFITM3) is an antiviral factor that plays important role in the host innate immune response against viruses. Previous studies have shown IFITM3 upregulated various tissues and organs after avian reovirus (ARV) infection, which suggests may be involved ARV infection. In this study, chicken gene was cloned analyzed bioinformatically. Then, of infection further explored. The results showed molecular weight approximately 13 kDa. This found to...
Loop-mediated isothermal amplification (LAMP) is a nucleic acid technique that can be used to amplify target genes at constant temperature, and it has several advantages, including convenience, specificity sensitivity. However, due the special interpretation methods of this technology for reaction results, all previously reported LAMP detection have been restricted identifying single target, which limits application technology. In study, we modified conventional include quencher-fluorophore...
Abstract Among the common methods used for antibody immobilization on electrode surfaces, which is best available option immunosensor fabrication? To answer this question, we first graphene-chitosan-Au/Pt nanoparticle (G-Chi-Au/PtNP) nanocomposites to modify a gold (GE). Second, avian reovirus monoclonal (ARV/MAb) was immobilized GE surface by using four methods, included glutaraldehyde (Glu), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/ N -hydroxysuccinimide (EDC/NHS), direct incubation...
The GX2020-019 strain of fowl adenovirus serotype 4 (FAdV-4) was isolated from the liver chickens with hydropericardium hepatitis syndrome in Guangxi Province, China, and purified by plaque assay three times. Pathogenicity studies showed that can cause typical FAdV-4 pathology, such as yellowing swelling. Four-week-old specific pathogen-free (SPF) inoculated virus at doses 103 median tissue culture infectious dose (TCID50), 104 TCID50, 105 106 107 TCID50 had mortality rates 0, 20, 60, 100,...
Interferon-alpha inducible protein 6 (IFI6) is an important interferon-stimulated gene. To date, research on IFI6 has mainly focused human malignant tumors, virus-related diseases and autoimmune diseases. Previous studies have shown that plays role in antiviral, antiapoptotic tumor-promoting cellular functions, but few the structure or function of avian IFI6. Avian reovirus (ARV) virus can exert immunosuppressive effects poultry. Preliminary expression upregulated various tissues organs...
Starting in 2015, the widespread prevalence of hydropericardium-hepatitis syndrome (HHS) has led to considerable financial losses within China’s poultry farming industry. In this study, pathogenicity assessments, whole-genome sequencing, and analyses were conducted on 10 new isolates novel genotype FAdV-4 during a HHS outbreak Guangxi Province, China, from 2019 2020. The results indicated that strains GX2019-010 GX2019-013 GX2019-015 GX2019-018 highly virulent, while strain GX2020-019...
H5, H7, and H9 are pivotal avian influenza virus (AIV) subtypes that cause substantial economic losses pose potential threats to public health worldwide. In this study, a novel triplex fluorescence reverse transcription-loop-mediated isothermal amplification (TLAMP) assay was developed in which traditional LAMP techniques were combined with probes for detection. Through innovative approach, of AIV can be simultaneously identified differentiated, thereby offering crucial technical support...
The H9N2 subtype of avian influenza virus (AIV) causes enormous economic losses and poses a significant threat to public health; the development vaccines against is ongoing. To study immunogenicity hemagglutinin (HA) protein, we constructed recombinant pET-32a-HA plasmid, induced HA protein expression with isopropyl β-D-1-thiogalactopyranoside (IPTG), verified it by SDS-PAGE Western blotting, determined sensitivity acid heat. Subsequently, mice were immunized purified immunization effect was...
Fowl adenovirus serotype 4 (FAdV-4) outbreaks have caused significant economic losses in the Chinese poultry industry since 2015. The relationships among viral structural proteins infected hosts are relatively unknown. To explore role of different parts fiber-1 protein FAdV-4-infected hosts, we truncated into fiber-1-Δ1 (73-205 aa) and fiber-1-Δ2 (211-412 aa), constructed pEF1α-HA-fiber-1-Δ1 pEF1α-HA-fiber-1-Δ2 then transfected them leghorn male hepatocyte (LMH) cells. After FAdV-4...