A5021030176- Osteoarthritis Treatment and Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Lower Extremity Biomechanics and Pathologies
- Total Knee Arthroplasty Outcomes
- Knee injuries and reconstruction techniques
- Adipose Tissue and Metabolism
- Exercise and Physiological Responses
- Bone health and osteoporosis research
- Bone and Joint Diseases
- Veterinary Equine Medical Research
- Tendon Structure and Treatment
- Inflammatory mediators and NSAID effects
- Diabetic Foot Ulcer Assessment and Management
- Bone Metabolism and Diseases
- Rheumatoid Arthritis Research and Therapies
- RNA Interference and Gene Delivery
- Orthopedic Infections and Treatments
- Hydrogels: synthesis, properties, applications
- Spaceflight effects on biology
- Vitamin D Research Studies
- Proteoglycans and glycosaminoglycans research
- Antimicrobial Peptides and Activities
- Bone fractures and treatments
- 3D Printing in Biomedical Research
- Musculoskeletal pain and rehabilitation
Montana State University
2016-2025
University of Washington
2015-2024
Montana State University System
2020
Complexo Hospitalario Universitario A Coruña
2019
University of California, Davis
2008-2018
GTx (United States)
2016
University of California San Diego Medical Center
2011
University of California, San Diego
2008-2011
Howard Hughes Medical Institute
2009
University of California Davis Medical Center
2008
Cellular uptake of the human immunodeficiency virus TAT protein transduction domain (PTD), or cell-penetrating peptide, has previously been surmised to occur in a manner dependent on presence heparan sulfate proteoglycans that are expressed ubiquitously cell surface. These acidic polysaccharides form large pool negative charge surface PTD binds avidly. Additionally, sulfated glycans have proposed aid interaction and other arginine-rich PTDs with membrane, perhaps aiding their translocation...
ObjectiveOsteoarthritis (OA) is a complex disease involving contributions from both local joint tissues and systemic sources. Patient characteristics, encompassing sociodemographic clinical variables, are intricately linked with OA rendering its understanding challenging. Technological advancements have allowed for comprehensive analysis of transcripts, proteomes metabolomes in tissues/fluids through omic analyses. The objective this review to highlight the achieved by studies enhancing our...
ABSTRACT The gut microbiome impacts bone mass, which implies a disruption to homeostasis. However, it is not yet clear how the affects regulation of mass and quality. We hypothesized that germ‐free (GF) mice have increased decreased toughness compared with conventionally housed mice. tested this hypothesis using adult (20‐ 21‐week‐old) C57BL/6J GF raised female male ( n = 6–10/group). Trabecular microarchitecture cortical geometry were measured from micro–CT femur distal metaphysis midshaft....
Abstract Articular cartilage functions to provide a low‐friction surface for joint movement many decades of life. Superficial zone protein (SZP) is glycoprotein secreted by chondrocytes in the superficial layer articular that contributes effective boundary lubrication. In both cell and explant cultures, TGF‐β1 IL‐1β have been demonstrated to, respectively, upregulate downregulate SZP levels. It was hypothesized friction coefficient could also be modulated these cytokines through regulation....
Osteoarthritis (OA) is a chronic joint disease with heterogenous metabolic pathology. To gain insight into OA-related metabolism, metabolite extracts from healthy (n = 11) and end-stage osteoarthritic cartilage 35) were analyzed using liquid chromatography–mass spectrometry metabolomic profiling. Specific metabolites pathways, including lipid amino acid differentially regulated in osteoarthritis-derived cartilage. The detected alterations acids lipids highlighted key differences bioenergetic...
We hypothesized that daily exercise promotes joint health by upregulating anti-inflammatory mediators via adaptive molecular and metabolic changes in the infrapatellar fat pad (IFP). tested this hypothesis conducting time-resolved analyses between 1 14 days of voluntary wheel running C57BL/6J mice. IFP structure cellularity were evaluated histomorphology, picrosirius red collagen staining, flow cytometry analysis stromal vascular fraction cells. Joint inflammation metabolism multiplex gene...
Viral infections induce major shifts in cellular metabolism elicited by active viral replication and antiviral responses. For the virus, harnessing evading changes that limit are essential for productive replication. In contrast, response to infection disrupts metabolic pathways prevent promote an state host cell neighboring bystander cells. This competition between virus results measurable differ depending on type, extracellular environment. The resulting can be observed analyzed using...
Obesity is a risk factor for osteoarthritis (OA), and leptin among the adipokines implicated in obesity-induced OA. However, specific role of OA severity pain not known. Using lipodystrophic (LD) mice, we show that fat-secreted factors are required knee development, implicating fat-cartilage cross-talk. Fat pad implantation or systemic restoration LD mice reintroduced structural pain, whereas leptin-deficient fat did change susceptibility. Isochronic parabiosis spatial transcriptomics...
Intracellular delivery of functional macromolecules using peptide transduction domains (PTDs) is an exciting technology with both experimental and therapeutic applications. Recent data indicate that PTD-mediated occurs via fluid-phase macropinocytosis involving intracellular pH drop to approximately 5. Nitrilotriacetic acid (NTA)-coordinated metals avidly bind hexahistidine-tagged macromolecules, including peptides proteins. Histidine's imidazole ring has a pK(a) 6, making this attractive...
Osteoarthritis occurs frequently after joint injury. Currently, osteoarthritis is diagnosed by radiographic changes that are typically found the disease has progressed to multiple tissues. The primary objective was compare potential metabolomic biomarkers of injury between synovial fluid and serum in a mouse model posttraumatic osteoarthritis. secondary gain insight into pathophysiology examining profiles Twelve-week-old adult female C57BL/6 mice (n = 12) were randomly assigned control, Day...
Articular cartilage is comprised of two main components, the extracellular matrix (ECM) and pericellular (PCM). The PCM helps to protect chondrocytes in from mechanical loads, but patients with osteoarthritis, weakened, resulting increased chondrocyte stress. As are responsible for synthesis maintenance, it important understand how loads affect cellular responses chondrocytes. Many studies have examined