A5090229853- Hepatitis B Virus Studies
- Hepatitis Viruses Studies and Epidemiology
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Liver Diseases and Immunity
- Viral Infections and Immunology Research
- Drug-Induced Hepatotoxicity and Protection
- Maternal and Perinatal Health Interventions
- Bacterial Identification and Susceptibility Testing
- Pharmacological Effects of Natural Compounds
- Celiac Disease Research and Management
- Streptococcal Infections and Treatments
- Bacterial Infections and Vaccines
- RNA Interference and Gene Delivery
- Pneumonia and Respiratory Infections
- Metastasis and carcinoma case studies
- Microscopic Colitis
- Animal Virus Infections Studies
- Helicobacter pylori-related gastroenterology studies
- Muscle metabolism and nutrition
- Phytochemistry and Bioactivity Studies
KIIT University
2023
North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences
2016-2022
All India Institute of Medical Sciences
2012-2021
Jawaharlal Institute of Post Graduate Medical Education and Research
2015
All India Institute of Medical Sciences Raipur
1990-2013
Boston Children's Hospital
2012
All India Institute of Medical Sciences Bhopal
1990-2000
International Centre for Genetic Engineering and Biotechnology
1992-1997
ICMR-National Institute of Virology
1992
Hepatitis E virus (HEV) is a major human pathogen in the developing world. In absence of an vitro culture system, very little information exists on basic biology virus. A small protein (approximately 13.5 kDa) unknown function, pORF3, encoded by third open reading frame HEV. We expressed pORF3 transiently transfected COS-1 and Huh-7 cells showed that it phosphoprotein which modified at serine residue(s). Deletion site-directed mutants were created to establish Ser-80 as phosphorylation site....
The profiles of patients with fulminant hepatic failure (FHF) from developing countries have not been reported earlier. current study was conducted prospectively, at a single tertiary care center in India, to document the demographic and clinical characteristics, natural course, causative profile FHF as well define simple prognostic markers these patients. Four hundred twenty-three consecutive admitted January 1987 June 1993 were included study. Each patient's serum tested for various...
Abstract Pregnant patients with acute liver failure (ALF) are believed to have a worse outcome than nonpregnant women and men ALF. However objective data supporting this supposition scant. Therefore, the current study compared outcome, complications, causes of ALF among pregnant girls age-matched boys One thousand fifteen consecutive in reproductive age group, admitted at All India Institute Medical Sciences, New Delhi, from January 1986 December 2006, were included study. A total 249...
Antituberculosis therapy (ATT)–associated acute liver failure (ATT-ALF) is the commonest drug-induced ALF in South Asia. Prospective studies on ATT-ALF are lacking. The current study prospectively evaluated magnitude, clinical course, outcome, and prognostic factors ATT-ALF. From January 1986 to 2009, 1223 consecutive patients were evaluated: ATT alone was cause 70 (5.7%) patients. Another 15 (1.2%) had simultaneous hepatitis virus infection. In 44 (62.8%) patients, prescribed empirically...
Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It positive-strand RNA with 7.5-kb polyadenylated genome consisting three open reading frames (ORFs). In absence an vitro culture system, replication and expression strategy HEV nature its encoded polypeptides are not well understood. We have expressed two ORFs constituting structural portion COS-1 cells by using simian 40-based vectors coupled transcription-translation system. show here that capsid protein,...
ABSTRACT Hepatitis E virus (HEV) is the etiological agent for viral hepatitis type E, which a major problem in developing world. Because HEV cannot be cultured vitro, very little information exists on mechanisms of gene expression and genome replication. positive-strand RNA with three potential open reading frames (ORFs), one (ORF2) postulated to encode capsid protein (pORF2). We earlier showed (S. Jameel, M. Zafrullah, H. Ozdener, S. K. Panda, J. Virol. 70:207–216, 1996) pORF2 ∼88-kDa...
ABSTRACT Hepatitis E virus (HEV) is an important etiological agent of epidemic and sporadic hepatitis, which endemic to the Indian subcontinent prevalent in most developing parts world. The infection often associated with acute liver failure high mortality, particularly pregnant women. In order develop methods intervention, it essential understand biology virus. This as no reliable vitro culture system available. We have constructed a cDNA clone encompassing complete HEV genome from...
<h3>Objective</h3> It is difficult to predict the outcome in patients with acute liver failure (ALF) using existing prognostic models. This study investigated whether early changes levels of dynamic variables can better than models based on static baseline variables. <h3>Design</h3> 380 ALF (derivation cohort n=244, validation n=136) participated a prospective observational study. The derivation was used identify predictors mortality. (ALFED) model constructed predictive remained...
The aetiological agents responsible for, and the outcome of, acute liver failure were investigated prospectively in 44 children (29 males, 15 females) attending a tertiary health care facility India. between ages of 2 months 13 years. Studies for viral infections other etiologies could be carried out 40 patients. Specific labels possible 35 (87.5%) Thirty (75%) had evidence hepatitis. Acute hepatitis E virus (HEV) infection was found total 18 children, with A (HAV) 16, B 5, C 1. Seven...
Abstract We studied epidemics of viral hepatitis occurring at three different places in India. One was a combined epidemic due to E virus (HEV) and A (HAV) infections. In this epidemic, HAV affected children below 10 years age, whereas HEV infected the young adult population. transmitted rhesus monkeys (Macaca mulata) confirmed by polymerase chain reaction (PCR) on bile from animals. Fecal material acutely patients one also found positive for RNA PCR. This may help confirming nature future...
Hepatitis E virus (HEV) causes enterically transmitted epidemic and sporadic viral hepatitis affecting millions of people in the developing world. Different geographical isolates HEV show a high degree homology at nucleotide amino acid levels. The approximately 7.2 kb RNA genome has three open reading frames which ORF1 is predicted to code for nonstructural polyprotein. expression, processing properties polyprotein have not been reported so far. In this study, complete was reconstructed from...
The magnitude and significance of perinatal transmission hepatitis B virus (HBV) were assessed in infants 8,575 women, whom 3.7% seropositive for HBV surface antigen (HBsAg). e (HBeAg) was found 7.8% these carriers, the antibody to HBeAg (anti-HBe) 30.1% them, HBsAg alone 62.1% them. estimated incidence positivity by 6 months age carrier women significantly (P less than .001) higher controls (18.6% vs 3.0%). Transmission most frequent (87.5%) if mother positive much so she anti-HBe (17.5%)...
Hepatitis E virus (HEV) is a common cause of acute on chronic liver failure (ACLF) in HEV hyperendemic regions with high mortality. Treatment for HEV-induced ACLF currently not available. Recently, efficacy ribavirin genotype 3 hepatitis patients has been reported; however, whether effective 1 infection yet known. The present study includes four treated region. Diagnosis was made by conventional criteria and as the confirmed detection RNA reverse transcriptase PCR. Ribavirin dose ranged from...
Summary. We investigated the virus–host interaction for hepatitis E virus (HEV) by performing competitive binding assays using in vitro assembled virus‐like particles (VLPs). used Escherichia coli expressed native capsid protein (pORF2) and its mutants with an attached Gly (5) ‐Ala (linker) reporter [enhanced green fluorescent (EGFP)/firefly luciferase (Fluc)]. Transmission electron microscopy nanoparticle tracking showed near uniform of approximately 30–35 nm diameter pORF2 VLPs 60–100...
Abstract Background and Aim: Acute on chronic liver failure (ACLF) because of precipitating factors (variceal bleed/infections) identifies cirrhotics at risk for high short‐term mortality. Information ACLF acute hepatic insults is lacking. The aim the study was to evaluate in their effect course outcome. Methods: In a prospective study, 213 consecutive patients were included. Etiology insult, frequency silent, overt disease (CLD), organ (OF), outcomes assessed. Prognostic models such as...