Mouse embryos segregate three different lineages during preimplantation development: trophoblast, epiblast and hypoblast. These differentiation processes are associated with restricted expression of key transcription factors (Cdx2, Oct4, Nanog Gata6). The mechanisms segregation have been extensively studied in the mouse, but not as well characterised other species. In human embryo, hypoblast has previously characterised. Here we demonstrate co-exclusive immunolocalisation Gata4 blastocysts,...

Mouse and rat embryonic stem cells can be sustained in defined medium by dual inhibition (2i) of the mitogen-activated protein kinase (Erk1/2) cascade glycogen synthase 3. The inhibitors suppress differentiation enable self-renewal pluripotent that are ex vivo counterparts naïve epiblast mature blastocyst. Pluripotent cell lines also derived from unipotent primordial germ via a poorly understood process epigenetic reprogramming. These termed (EG) to denote their distinct origin. Here we...

Germline-competent embryonic stem cells (ESCs) have been derived from mice and rats using culture conditions that include an inhibitor of glycogen synthase kinase 3 (GSK3). However, rat ESCs remain susceptible to sporadic differentiation. Here, we show unsolicited differentiation is attributable overinhibition GSK3. The self-renewal effect inhibiting GSK3 mediated via β-catenin, which abrogates the repressive action TCF3 on core pluripotency genes. In ESCs, however, inhibition also leads...

Introduction: The study investigated the impact of museum object handling sessions on hospital clients receiving occupational therapy in neurological rehabilitation and an older adult acute inpatient mental health service. Methods: research used a qualitative approach based objectivist constructionist methods, from which themes typical were derived. Results: Themes emerging detailed analysis discourse involving (n = 82) healthcare staff 8) comprised: distraction decreasing negative emotion;...

The ability of cultured pluripotent cells to contribute the germline chimaeric animals is essential their utility for genetic manipulation. In three years since rat embryonic stem (ES) were first reported anticipated proliferation genetically modified models from this new resource has not been realised. Culture instability, karyotypic anomalies, and strain variation are postulated poor colonisation capacity. resolution these issues bring cell-based manipulation technology in level efficiency...

This work investigates the use of micro-computed tomography (micro-CT) for identification New Zealand plant leaf material from artefacts. Micro-CT was explored as a result difficulties in preparing transverse sections aged artefacts to compare with reference slides microscopic species. Plant names te reo Māori (Māori language) are followed by English name and botanic name, subsequently referred names. The three plants investigated (harakeke, flax, Phormium tenax, J. R. Forst & G. Forst; tī...

This qualitative study explores the dynamic changes that occur in family interaction patterns when Alzheimer's disease is present. Semi-structured interviews were conducted with 15 participants who have a member disease. Using modified analytic induction, guided by dimensions of Family Fundamental Interpersonal Relations Orientation (FIRO) Model, shared how affected structure, control dynamics, and intimacy among members. Findings demonstrate (a) families reorganize restructure based on...

We explore the use of cinematic "pre-visualization" (previs) techniques as a rapid ideation and design futuring method for human computer interaction (HCI) research. Previs approaches, which are widely used in animation film production, digital tools to create medium-fidelity videos that capture richer interaction, motion, context than sketches or static illustrations. When method, previs can facilitate rapid, iterative discussions reveal tensions, challenges, opportunities new performed...

Art is not Research. Research Art. a multimedia, multi-site participatory installation by collective of artists and researchers from Calgary, Toronto, Lancaster; it informed these contexts. It reflects the tensions between how "participants" are treated in art interaction research. offers framework through which we can explore epistemologies might evolve blending Visitors download paper to read, critically reflect on relationship research, experientially engage with material series creative...

Chronic obstructive pulmonary disease (COPD) has been identified as the fourth leading cause of death. The primary purpose this article is to discuss risk factors for COPD and identify at-risk populations. A secondary report findings a recent survey nurse practitioners (NPs) in Colorado regarding their knowledge about COPD. Data sources list registered NPs State was obtained from Board Nursing. Surveys were sent 2916 assess COPD.Although response rate less than 10%, highlighted that there...

In Brief For APNs teaching in NP programs, the importance of faculty practice is clear. order to have expertise, one must be clinically up-to-date.

Although hardly a recent phenomenon, the battered woman has still not been clearly recognizable presence to primary caregivers. Unawareness of actual victims stems from an incomplete understanding reality "battered wife syndrome," lack knowledge about various forms abuse and silence themselves. The victim rarely reports care provider without being asked. She does, however, present with common psychosomatic complaints, such as choking, gastrointestinal disorders or nervousness. Her injuries...

ABSTRACT The "graying of America" will have profound effects on the health care delivery system. With shrinking amounts dollars, nurses and primary providers need to promote maintenance disease prevention strategies that foster healthier aging. This study was designed examine promotion family practice physicians residents. target population a sample 201 women age 60 older. issues were counseling about hormone replacement therapy (HRT), urinary incontinence (Ul), immunizations. results...

Machine learning and predictive algorithms find patterns in large stores of data make predictions which corporations governments use to support decision-making. Yet, the system's representation reality can be more influential outcomes than complexities daily life. They become problematic when they undermine inclusivity public decision making, their perpetuates social or economic inequality.