To investigate the frequencies and bacterial load of three species periodontal bacteria in samples from oropharyngeal cancer patients versus healthy individuals.This is a case-control study based on biopsies collected tumor tissues obtained with squamous cell carcinoma between 2016 2017 shed oral mucosal epithelial cells that were controls using Cepimax® brush, carrying out several brushings towards posterior third edge tongue cheek. Porphyromonas gingivalis, Tannerella forsythia Prevotella...

Bone tissue regeneration strategies have incorporated the use of natural polymers, such as hydroxyapatite (nHA), chitosan (CH), gelatin (GEL), or alginate (ALG). Additionally, platelet concentrates, platelet-rich fibrin (PRF) been suggested to improve scaffold biocompatibility. This study aimed develop scaffolds composed nHA, GEL, and CH, with without ALG lyophilized PRF, evaluate scaffold's properties, growth factor release, dental pulp stem cells (DPSC), osteoblast (OB) derived from DPSC...

Objective The objective of this study is to investigate the expression and concentration ligand receptor activator NFκB (RANKL) osteoprotegerin (OPG) in human periodontal ligament (hPDL) with orthodontic forces different magnitudes. Methods Right premolars 32 patients were loaded 4oz or 7oz force for 7 days. Left first not loaded. After days, extracted treatment as indicated. OPG RANKL mRNA expressions measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), ELISA...

Abstract Introduction: Human papilloma virus (HPV) and oral bacteria capable of acetaldehyde production from ethanol, such as Streptococcus anginosus, Prevotella melaninogenica, Fusobacterium naviforme are among oropharyngeal squamous cell carcinoma (OSCC) infectious risk factors. Objective: Determine associations with HPV S. P. F. in patients without OSCC. Methods: Presence HPV-16 was determined 26 OSCC by conventional PCR simultaneous presence quantification through q-PCR. Statistical...

Chronic periodontitis is a multifactorial infectious disease associated with Gram-negative strict anaerobes which are immersed in the subgingival biofilm. Porphyromonas gingivalis, an important periodontal pathogen, frequently detected patients chronic periodontitis. Although isolates of P. gingivalis tend to be susceptible most antimicrobial agents, relatively little information available on its vitro susceptibility. The aim this study was determine frequency and assess susceptibility terms...

Opportunistic intestinal parasites are a common cause of diarrhea in HIV-infected patients. To determine the prevalence microsporidia and other opportunistic infecting HIV patients Bogotá, Colombia, 115 were examined for these infections during year 2001. The institution sample percent from each as follows: Santa Clara Hospital, 33.0%; San Pedro Claver, 20.0%; Simón Bolívar 14.8%; José 13.9%; Central de la Policía 6.1%; Compensar, 5.2%; Colombian League against AIDS, 2.6%; Ignacio 2.6%,...

Background: Conventional periodontal therapy relies on bone regeneration strategies utilizing scaffolds made of diverse materials, among which collagen, to promote cell adhesion and growth. Objective: To evaluate ligament fibroblast (HPdLF) viability for purposes hydroxyapatite containing collagen (HAp-egg shell) combined with polylactic acid−polyglycolic acid copolymer (PLGA) Platelet-Rich Fibrin (PRF). Methods: Four variations the HAp-egg shell were used seed HPdLF 24 h through a live/dead...

Cancer of the uterine cervix (CC) is second most common cancer in women worldwide. In Colombia, CC frequent among entire population and first aged between 15 44 years, with an estimated incidence 24.9 cases/100,000 inhabitants. The main risk factor infection one or more high-risk human papillomavirus (HPV) types. aim this study was to estimate genotype-specific prevalence DNA patients cervical pathology using multiplex PCR Luminex xMAP technology. addition, we compared genotyping Reverse...

Abstract Objective To identify the characteristics of oral microbiota and relationship dental caries periodontal status in patients aged 0 to 18 years with non-syndromic cleft lip palate (CLP). Materials methods A systematic review literature was carried out. Five databases were consulted, including publications English, Spanish Portuguese. The evaluations quality observational studies experimental out Newcastle–Ottawa scale CONSORT guidelines, respectively. risk bias determined using Rev...

This study investigates how varying concentrations of hydroxyapatite (OHAp) and the addition gelatin influence ionic crosslinking time alginate-based bioinks, as well shear stress experienced by neonatal human dermal fibroblasts (HDFn) during extrusion. These factors are crucial for validating bioinks developing viable 3D bioprinted models. Four bioink formulations were created with a 50/50 ratio alginate to gelatin, incorporating different calcium phosphate (0%, 1%, 5%, 10%). The...

To identify human papillomavirus (HPV), Epstein-Barr virus (EBV), and Candida albicans in oral leukoplakia with different degrees of dysplasia.An observational, cross-sectional, descriptive study was performed using 30 formalin-fixed paraffin-embedded tissues from patients clinical suspicion confirmed diagnosis dysplasia. Histological analyses were by two pathologists (interobserver) dysplasias classified as mild, moderate, or severe. Conventional PCR used to detect HPV EBV viruses C....

Infections of the oral cavity have a broad microbial etiological profile that varies according to each microenvironment in mouth. Such infections often require antimicrobial treatment, which can lead development resistance. There is thus need find new therapeutic strategies based on natural plant-derived compounds. The aim this study was determine phytochemical nuclei and effect Anacardium excelsum leaf stem extracts, fractions derived from extract, against Streptococcus mutans ATCC 25175,...

<p><strong>Introducción.</strong> Uno de los factores riesgo del carcinoma células escamosas en la cavidad oral es infección por el virus papiloma humano (HPV), cuyas prevalencias dependen región geográfica.<br /><strong>Objetivo.</strong> Identificar tipos más frecuentes cáncer bucal, sus niveles expresión y estado físico genoma viral.<br /><strong>Materiales métodos.</strong> Se seleccionaron 46 pacientes que asistían a servicios...

Antecedentes: el cáncer escamocelular de cabeza y cuello puede ser causado principalmente por un alto consumo cigarrillo alcohol. Sin embargo, cerca 30 % los tumores ocasionado una infección virus del papiloma humano (VPH), en la orofaringe. En última década, frecuencia este tipo tumor se ha incrementado mundialmente. Propósito: analizar las características diferencias que presentan orofaringe asociados o no a VPH. Métodos: revisó literatura sobre asociación entre VPH, su pronóstico....

The development of polyclonal antibodies requires laboratory animals, as the usually rabbits, which are bled for obtaining immunoglobulins. Hens an alternative to developing IgY antibodies.In current study, hens were used develop anti-Giardia duodenalis and evaluate five protocols its purification.Three immunized intramuscularly with Colombian Giardia isolates trophozoites on day 0, 15, 30, 45, 60, 90 120. hen eggs collected before each immunization, purified from yolk by delipidation (D)...

When the normal microbiota-host interactions are altered, commensal microbial community evolves to a dysbiotic status resulting in some species becoming pathogenic and acting synergistically development of local systemic diseases, including cancer. Advances genetics, immunology microbiology during last years have made it possible gather information on oral gastrointestinal microbiome its interaction with host, which has led better understanding interrelationship between microbiota There is...

Objetivo: genotipificar el VPH en muestras de pacientes con diferentes grados histopatológicos lesión cervical.Materiales y métodos: estudio corte transversal cepillado cervical mujeres lesiones cervicales histopatológicas la ciudad Bogotá año 2009. Se utilizó Luminex® xMAP® una técnica altamente sensible, reproducible automatizada, Reverse Line Blot (RLB).Resultados: las infecciones por los tipos 16 18 fueron más frecuentes todos patología del cérvix. Las mixtas predominaron un 78,04% casos...

<strong>ABSTRACT<em>. Background:</em></strong><em> </em>Growth factors used in health treatments can be obtained from a first-generation source called platelet-rich plasma. The variety of protocols to prepare PRP produces variable results regarding activation time and its effects on cell proliferation viability. <strong><em>Purpose:</em></strong><em> </em>To evaluate viability periodontal ligament fibroblasts...

Objetivos: investigar los biotipos de S. mutans aislados niños con y sin caries dentaly determinar su susceptibilidad antimicrobiana. Métodos: este estudio observacionaldescriptivo incluyó 206 entre 3 5 años dos preescolares en Bogotá, D.C., Colombia. Después aislamiento agar Mitis Salivarius Bacitracina, lascepas se identificaron por pruebas bioquímicas biotipificaron elsistema api-ZYM. En todos aislamientos determinó el método diluciónenvagar la concentración inhibitoria mínima (CIM)...

Saliva samples are important for diagnosis, because they noninvasive and easy to acquire. The objective of this study was investigate the value saliva have in detecting SARS-CoV-2 comparison nasal swabs a new system named CovidCheck. A standard methodology identified virus 185 nasopharyngeal revealing sensitivity, specificity positive negative predictive values 82,100,100 94.67%, respectively samples. Viral presence with CovidCheck evaluated 67 68, 81, 68 81%, respectively. In conclusion,...

Saliva samples are important for diagnosis, because they noninvasive and easy to acquire. The objective of this cross-sectional study was investigate the value saliva have in detecting SARS-CoV-2 comparison nasal swabs a new system named CovidCheck. A standard methodology identified virus 185 nasopharyngeal revealing sensitivity, specificity positive negative predictive values 82,100,100 94.67%, respectively samples. Viral presence with CovidCheck evaluated 67 68, 81, 68 81%, respectively....