Pradhyumna Kumar Singh

ORCID: 0000-0003-0780-8312
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About
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Research Areas
  • Insect Resistance and Genetics
  • Transgenic Plants and Applications
  • Plant tissue culture and regeneration
  • Plant Virus Research Studies
  • Toxin Mechanisms and Immunotoxins
  • CRISPR and Genetic Engineering
  • Insect and Pesticide Research
  • Research in Cotton Cultivation
  • Insect-Plant Interactions and Control
  • Plant biochemistry and biosynthesis
  • Microbial bioremediation and biosurfactants
  • Alzheimer's disease research and treatments
  • Photosynthetic Processes and Mechanisms
  • Insect symbiosis and bacterial influences
  • Glycosylation and Glycoproteins Research
  • Protein Kinase Regulation and GTPase Signaling
  • RNA and protein synthesis mechanisms
  • Ion Transport and Channel Regulation
  • Insect Pest Control Strategies
  • Plant Molecular Biology Research
  • Neuroinflammation and Neurodegeneration Mechanisms
  • Nanoparticles: synthesis and applications
  • Plant Disease Resistance and Genetics
  • Biochemical and biochemical processes
  • Parkinson's Disease Mechanisms and Treatments

National Botanical Research Institute
2015-2024

Academy of Scientific and Innovative Research
2014-2024

Council of Scientific and Industrial Research
2010-2023

Immunovaccine (Canada)
2010-2011

Flavonoids synthesized by the phenylpropanoid pathway participate in myriad physiological and biochemical processes plants. Due to diversity of secondary transformations complexity regulation branched pathways, single gene strategies have not been very successful enhancing accumulation targeted molecules. We expressed an Arabidopsis (Arabidopsis thaliana) transcription factor, AtMYB12, tobacco (Nicotiana tabacum), which resulted enhanced expression genes involved pathway, leading severalfold...

10.1104/pp.109.150979 article EN PLANT PHYSIOLOGY 2010-02-26

Background The concept of DNA barcoding for species identification has gained considerable momentum in animals because fairly successful using cytochrome oxidase I (COI). In plants, matK and rbcL have been proposed as standard barcodes. However, complex genera is a challenging task. Methodology Principal Findings We investigated the discriminatory power four reportedly most promising plant loci (one from nuclear genome- ITS, three plastid trnH-psbA, matK) Indian Berberis L. (Berberidaceae)...

10.1371/journal.pone.0013674 article EN cc-by PLoS ONE 2010-10-27

Background Expression of double strand RNA (dsRNA) designed against important insect genes in transgenic plants have been shown to give protection pests through interference (RNAi), thus opening the way for a new generation insect-resistant crops. We earlier compared efficacy dsRNAs/siRNAs, number target genes, growth whitefly (Bemisia tabaci) upon oral feeding. The v-ATPase subunit A (v-ATPaseA) coding gene was identified as crucial target. now report effectiveness tobacco expressing siRNA...

10.1371/journal.pone.0087235 article EN cc-by PLoS ONE 2014-03-03

Abstract Background Cotton ( Gossypium hirsutum L.) is a major fiber crop that grown worldwide; it faces extensive damage from sap-sucking insects, including aphids and whiteflies. Genome-wide transcriptome analysis was performed to understand the molecular details of interaction between L. pests, namely Aphis gossypii (Aphid) Bemisia tabacci (Whiteflies). Roche’s GS-Titanium used sequence transcriptomes cotton infested with whiteflies for 2 h 24 h. Results A total 100935 contigs were...

10.1186/1471-2164-14-241 article EN cc-by BMC Genomics 2013-04-11

Isoniazid is used either alone or in combination with other drugs for the treatment of tuberculosis. It also prevention Chronic may cause severe liver damage leading to acute failure. The mechanism through which causes investigated. generates reactive oxygen species and induces apoptosis Hep3B cells. antioxidative apoptotic genes increase mRNA expression protein levels Whole genome analysis cells treated has resulted differential various playing prime role regulation apoptotic,...

10.1016/j.redox.2015.06.020 article EN cc-by-nc-nd Redox Biology 2015-07-08

Abstract The applicability of the statistical tools coupled with artificial intelligence techniques was tested to optimize critical medium components for production extracellular cholesterol oxidase (COD; an enzyme commercial interest) from Streptomyces rimosus MTCC 10792. initial component screening performed using Placket-Burman design yeast extract, dextrose, starch and ammonium carbonate as significant factors. Response surface methodology (RSM) attempted develop a model coefficient...

10.1038/s41598-018-29241-9 article EN cc-by Scientific Reports 2018-07-13

Nucleotide positions conserved on the 3' side of initiator codon ATG and corresponding N-terminal amino acid residues in a number highly abundant plant proteins were identified by computational analysis dataset expressed genes. The reporter genes uidA gfp modified to introduce these features. Insertion GCT TCC after augmented expression for both insertion each successive improved beta-glucuronidase (GUS) an incremental fashion transient transformation tobacco (Nicotiana tabacum) leaves....

10.1104/pp.126.4.1630 article EN PLANT PHYSIOLOGY 2001-08-01

Tomatoes are one of the most consumed crops in whole world because their versatile importance dietary food as well many industrial applications. They also a rich source secondary metabolites, such phenolics and flavonoids. In present study, we described method to produce these compounds from hairy roots tomato (THRs). Agrobacterium rhizogenes strain A4 was used induce explants. The Ri T-DNA confirmed by polymerase chain reaction amplification rolC gene. Biomass accumulation root lines...

10.1021/jf405695y article EN Journal of Agricultural and Food Chemistry 2014-03-03

Ormeloxifen is a nonsteroidal selective estrogen receptor modulator (SERM) and has been shown to possess anticancer activities in breast uterine cancer. Here, we show that ormeloxifen induces apoptosis dose-dependent manner variety of leukemia cells, more strikingly K562. 2-DE-gel electrophoresis K562 cells induced with showed 57 30% proteins belong cell-cycle pathways, respectively. Our data demonstrate ormeloxifen-induced involves activation extracellular signal-regulated kinases (ERKs)...

10.1002/pmic.201000720 article EN PROTEOMICS 2011-02-14
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