A5068519671- Plant tissue culture and regeneration
- Coffee research and impacts
- Transgenic Plants and Applications
- Plant Virus Research Studies
- Cocoa and Sweet Potato Agronomy
- Growth and nutrition in plants
- Plant Disease Management Techniques
- CRISPR and Genetic Engineering
- Organic Food and Agriculture
- Plant-Microbe Interactions and Immunity
- Seed Germination and Physiology
- Nematode management and characterization studies
- Energy and Environment Impacts
- Plant Molecular Biology Research
- Rice Cultivation and Yield Improvement
- Plant Stress Responses and Tolerance
- Chromosomal and Genetic Variations
- Agricultural Innovations and Practices
- Pineapple and bromelain studies
- Plant Gene Expression Analysis
- Agriculture and Rural Development Research
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Agroforestry and silvopastoral systems
- Garlic and Onion Studies
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2013-2024
Université de Montpellier
2018-2024
Institut de Recherche pour le Développement
2011-2024
Diversité, adaptation et développement des plantes
2022-2024
Agropolis International
2011-2019
Laboratoire des Interactions Plantes Micro-Organismes
2016-2019
Diversité, génomes et interactions micro-organismes-insectes
2019
Interactions Arbres-Microorganismes
2015
Embryogenic suspensions that involve extensive cell division are risky in respect to genome and epigenome instability. Elevated frequencies of somaclonal variation embryogenic suspension-derived plants were reported many species, including coffee. This problem could be overcome by using culture conditions allow moderate proliferation. In view true-to-type large-scale propagation C. arabica hybrids, suspension protocols based on low 2,4-D concentrations short proliferation periods developed....
Long-term cell cultures were used in coffee to study the cytological, genetic and epigenetic changes occurring during culture ageing. The objective was identify mechanisms associated with somaclonal variation (SV). Three embryogenic lines established Coffea arabica (2n = 4x 44) somatic seedlings regenerated after 4, 11 27 months. Phenotyping AFLP, MSAP, SSAP molecular markers performed on 199 124 plants, respectively. SV only observed from 27-month-old cultures, affecting 30 94 % of...
Abstract Conventional American cultivars of coffee are no longer adapted to global warming. Finding highly productive and stable in different environments without neglecting quality characteristics has become a priority for breeders. In this study, new Arabica F1 hybrids clones were compared conventional varieties seven contrasting environments, yield, rust incidence volume the canopy. The was assessed through size, weight 100 beans, biochemical analysis (24 aroma precursors 31 volatiles...
Abstract Background Somatic embryogenesis (SE) is one of the most promising processes for large-scale dissemination elite varieties. However, many plant species, optimizing SE protocols still relies on a trial and error approach. We report first global scale transcriptome profiling performed at all developmental stages in coffee to unravel mechanisms that regulate cell fate totipotency. Results RNA-seq 48 samples (12 × 4 biological replicates) generated 90 million high quality reads per...
Coffea arabica F1 hybrids derived from crosses between wild Sudan-Ethiopian and American cultivars propagated by somatic embryogenesis have been obtained in Central America. These new considerably enhanced the genetic diversity of coffee region. We conducted 15 trials to assess whether using represents substantial progress terms productivity agroforestry full-sun cropping systems. The germplasm was grown same conditions as best cultivar (homozygous pure lines). results showed that yields...
In Coffea arabica L., the development of direct sowing somatic embryos (SE) in planting substrate, with subsequent nursery production plants, has promoted industrialization embryogenesis. However, plant conversion rates are still low and require improvements to enhance cost-effectiveness commercial micropropagation. With aim improving regeneration from SE, we studied morphological histological criteria water characteristics during germination zygotic (ZE) SE. At cotyledonary stage, SE...
In general, the current industrial somatic embryogenesis (SE) propagation processes for coffee are costly because they not productive enough. We show that SE-derived plantlets from C. arabica hybrids were temporarily − between 10 and 25 weeks of development in nursery able to root with a high success rate (up 90%) whatever genotype tested, before gradually losing capacity. took advantage this transient rooting capacity, probably due rejuvenation process occurring during SE, establish new...
In the present paper, we evaluated implementation of a seed production system based on exploitation male sterility coffee. We studied specifically combination between CIR-SM01 and Marsellesa® (a Sarchimor line), which provides hybrid population called Starmaya. demonstrated that establishment garden under natural pollination is possible produces sufficient amount seeds to be multiplied efficiently economically. As expected for F1 hybrid, performances Starmaya are highly superior conventional...
Adapting to climate change in vulnerable coffee regions is crucial maintain rural livelihoods. Among the solutions, breeding strategies aim produce varieties with higher output performance than traditional while reducing competition for land. This paper investigates of hybrid (e.g., Starmaya and Centroamericano – H1), introgressed Marsellesa obatá) Caturra Villa Sarchi) varieties. By using plot-level panel data among commercial farms Costa Rica, we estimate three pooled ordinary least...
The effects of the environment and genotype in coffee bean chemical composition were studied using nine trials covering an altitudinal gradient [600-1100 m above sea level (a.s.l.)] with three genotypes Coffea arabica northwest mountainous region Vietnam. impacts climatic conditions on physical characteristics assessed.We showed that had a significant effect density all compounds. was stronger than genotype-environment interaction for cafestol, kahweol, arachidic (C20:0), behenic acid...
Somatic embryogenesis (SE) is one of the most promising processes for large-scale dissemination elite varieties. However, many plant species, optimizing SE protocols still relies on a trial-and-error approach. Using coffee as model plant, we report here first global analysis metabolome and hormone dynamics aiming to unravel mechanisms regulating cell fate totipotency. Sampling from leaf explant dedifferentiation until embryo development covered 15 key stages. An in-depth statistical...
Of all the possible micropropagation techniques, vegetative propagation by somatic embryogenesis is far most promising one for rapid, large-scale dissemination of elite individuals. Yet, to date, examples processes applied at an industrial scale are very few and between. There many complications. They usually involve a major genotypic effect, particularly obtaining embryogenic tissues, or related quality regenerated embryos, incidence somaclonal variation and, more generally, lack...
Over 80 % of all Coffea arabica - a species African origin is produced in Latin America. The gene pool C. arabica, which was introduced America the 19th century, includes only two relatively similar varieties (Bourbon and Typica). Despite this very weak pool, researchers have managed to develop pure line that are well adapted crop intensification (1950-1980) produce coffee recognised standard quality. With advent rust, resistance genes diploid canephora were transferred these improved...
Abstract The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using extract plants tissues and demonstrate that this extraction method works not only at room temperature but also elevated temperatures provides the simplest most effective single-step undegraded directly tropical in field. complex field environment opens up way for studying gene-environment interactions transcriptome level decipher regulatory network involved multiple-stress responses.
Breeding programs have developed high-yielding Coffea arabica F1-hybrids as an adaptation against adverse conditions associated with climate change. However, theresponse to drought of coffee F1 hybrids has seldom been assessed.
Societal Impact Statement Despite strong historical declines, Guadeloupe and Haiti's coffee sectors remain important to rural communities' livelihood resilience. Coffee also holds value as part of the islands' legacy cultural identities. Furthermore, it is often grown in agroforestry systems providing ecosystem services, which will become more these vulnerable islands work adapt a changing climate. Current efforts revitalize farms target strategically specialty markets would benefit from...