Herein, we report a bottom-up approach to assemble series of stochastic DNA walkers capable probing dynamic interactions occurring at the bio–nano interface. We systematically investigated impact varying interfacial factors, including intramolecular interactions, orientation, cooperativity, steric effect, multivalence, and binding hindrance on enzymatic behaviors interfaces spherical nucleic acids. Our mechanistic study has revealed critical roles various factors that significantly alter...

Based on selective and sensitive determination of Hg(2+) released from mercury complex by cold vapor generation (CVG) atomic fluorescence spectrometry (AFS) using SnCl2 as a reductant, novel label-free separation-free strategy was proposed for DNA protein bioassay. To construct the bioassay platform, an Hg(2+)-mediated molecular beacon (hairpin) without labeling but possessing several thymine (T) bases at both ends employed probe. It is well-known that could trigger formation hairpin...

A selective reduction-based method was proposed for the sensitive detection of iodide and melamine using chemical vapour generation (CVG) coupled with atomic fluorescence spectrometry (AFS).

Stimuli-responsive nanomachines are attractive tools for biosensing, imaging, and drug delivery. Herein, we demonstrate that the orientation of macromolecules subsequent dynamic interactions at biomolecule-nanoparticle (bio-nano) interfaces can be rationally controlled to engineer stimuli-responsive DNA nanomachines. The success this design principle was demonstrated by engineering a series antibody-responsive walkers capable moving persistently on three-dimensional track made functionalized...

<title>Abstract</title> This article proposes a method based on elastic wave data reconstruction and denoising to solve the problem of defect localization in grounding grid down conductor flat steel. Due environmental factors, cross media propagation, interference from excitation sources, noise is coupled into original signal during acquisition, seriously affecting processing. To this problem, paper uses Particle Swarm Optimization Orthogonal Matching Pursuit (PSO-OMP) algorithm reconstruct...

Cells take advantage of the spatial organization to accelerate reaction kinetics diverse components within a crowded intracellular environment. Inspired by this, we hereby designed principle constraint overcome limitations response in DNAzyme-powered DNA nanomachines. First, proposed type-1 spatially constrained nanomachines (scDN-1) co-localizing aptamer probe and power unit (DNAzyme), allowing accomplish faster cyclic cleavage DNAzyme as intramolecular reactions. To expand scDN into...

Colloidal nanoparticle biosensors capable of on-particle biocatalysis are powerful tools for amplified detection biomolecules. The development and practical uses such concentric amplifiers can be complicated because the biorecognition that involves varying interfacial factors at biomolecule-nanoparticle interfaces. Herein, we reason a biosensor equipped with an in-solution element may better fabricated, predicted, controlled, performed. shall also streamlined so overall analytical kinetic...

Herein, a DNAzyme-powered nanomachine responsive to multiple hepatocellular carcinoma (HCC)-related miRNAs derived from clinical samples was designed. Initially, three types of nanomachines were constructed with dye molecule [(fluorescein (FAM), tetramethylrhodamin (TMR), and Cyanine 5 (Cy5)]-labeled DNA-RNA chimeric substrates specific recognized probe for the corresponding target. Once target captured by two recognizing probes, DNA initiated, leading hybridization between DNAzyme...

Herein, we describe two homogeneous conversion systems that can convert protein recognition into the release of predesigned output DNA for activation nanomachines. With high efficiency, this strategy allows us to initiate nanomachines respond any proteins, opening up possibility applying in clinical practice.

To obtain enhanced physical and biological properties, various nanoparticles are typically assembled into hybrid through the binding of multiple homologous DNA strands to their complementary counterparts, commonly referred as homomultivalent assembly. However, poor affinity limited controllability disassembly restrict assembly yield dynamic functionality nanoparticles. achieve a higher flexible choice, we utilized paired heteromultivalency construct demonstrate excellent characteristics...

DNA-based logic computing potentially for analysis of biomarker inputs and generation oligonucleotide signal outputs is great interest to scientists in diverse areas. However, its practical use sensing multiple biomarkers limited by the universality robustness. Based on a proximity assay, lanthanide encoded logically gated micromachine (LGM-Ln) was constructed this work, which capable responding multiplex biological matrices. Under function controls triggered Boolean "AND" algorithm, it...

Isothermal exponential amplification technology has rarely been fabricated as a universal sensing platform for the detection of proteins. To broaden their application, we have developed strategy, named protein-recognition-initiated reaction (PRIEAR) using protein recognition to induce DNA assembly, which converts events into ssDNA amplicons and combines two-stage achieve amplification. Taking advantage this principle, diverse biomarkers can be quantified at sub-picomolar concentrations in...

Using the principle of binding-induced DNA strand displacement (BINSD), a DNAzyme-powered nanomachine biosensor for multiple biomarkers via magnetic beads-based signal conversion was designed. This sensor can convert biomarker recognition into release predesigned output nucleic acids tagged with streptavidin proteins (SA-DNA) activation nanomachines. In general, we adopted complementary base pairing rules and affinity ligand specific recognition, three types systems were constructed that...

A DNAzyme-powered micromachine with anti-interfering properties and displaying resistance to being inhibited by biological matrices was built. This able respond a specific target in high-concentration serum or whole blood.

In this paper, the soybean straw was modified into activated carbon adsorbent by chemical activation using zinc chloride as activator, and influence of pH on adsorption effect kinetic characteristics reactive brilliant red K-2BP under different dosage, oscillation speed temperature were investigated static experiments.In addition, date respectively fitted pseudo-first-order equation, pseudo-second-order equation intra particle diffusion equation.It found that could reach best decolorization...

A comparison of Geant4 physics lists is conducted in the calculation total absorbed dose, boron and non-boron dose phantom, depth-dose, depth-dose along beam axis for neutrons a range 0.0253 eV to 10 MeV. Physics processes are included neutrons, photons, charged particles, calculations secondary particles. The results obtained from QBBC, QGSP_BERT, neutron high precision with without S(α, β) data compared FLUKA values. Neutron showed best agreement studied energy range.

Seven compounds, including two flavanones, dihydrokaempferol ( 1 ) and naringenin 2 ), five terpenoids, boscartol A 3 3,7-dioxo-tirucalla-8,24-dien-21-oic acid 4 3α-acetoxyl-7-oxo-tirucalla-8,24-dien-21-oic 5 11-keto-β-boswellic 6 acetyl-11-keto-boswellic 7 have been purified by high-speed counter-current chromatography (HSCCC) from olibanum. For the separation, 250 mg of crude extract, 3.1 (95.2% purity), 2.7 (96.1% 9.1 (96.7% 4.5 (95.3% 5.4 (96.3% 48.1 (96.8% 45.5 (98.1% purity) were...

Abstract Background Extracorporeal membrane oxygenation (ECMO) is increasingly being used for critically ill patients with cardiopulmonary failure. Air in the ECMO circuit an emergency, a rare but fatal complication. Case presentation: We introduce case of 76-year-old female who suffered from cardiac arrest complicated severe trauma and was administered veno-arterial extracorporeal oxygenation. In managing patient ECMO, air entered circuit, which had not come out nor folded or broken....

Extracorporeal membrane oxygenation (ECMO) is increasingly being used for critically ill patients with cardiopulmonary failure. Air in the ECMO circuit an emergency, a rare but fatal complication.

Background: We sought to describe our experience and outcomes of a zone 0 landing physician-modified aortic stent for the treatment arch pathologies late after open ascending replacement. Methods: This study included consecutive patients with diseases treated total endovascular repair using different techniques. The indication was agreed on in multidisciplinary discussion. Study end points were technical success, early outcomes. Results: From November 2018 May 2022, 16 underwent pathologies....