A5082638904- Cell Adhesion Molecules Research
- dental development and anomalies
- Cleft Lip and Palate Research
- Proteoglycans and glycosaminoglycans research
- Corneal Surgery and Treatments
- Cellular Mechanics and Interactions
- Osteoarthritis Treatment and Mechanisms
- Wound Healing and Treatments
- Bone and Dental Protein Studies
- Oral microbiology and periodontitis research
- Hedgehog Signaling Pathway Studies
- Periodontal Regeneration and Treatments
- Peptidase Inhibition and Analysis
- Endodontics and Root Canal Treatments
- Protease and Inhibitor Mechanisms
- Silk-based biomaterials and applications
- Bone Metabolism and Diseases
- Corneal surgery and disorders
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- Signaling Pathways in Disease
- Wnt/β-catenin signaling in development and cancer
- Pesticide Exposure and Toxicity
- Glaucoma and retinal disorders
- Mesenchymal stem cell research
Texas A&M University
2010-2024
The University of Texas at Dallas
2024
Australian Regenerative Medicine Institute
2023
Monash University
2023
National Cancer Institute
2023
The University of Texas Southwestern Medical Center
2002-2020
Medical University of Vienna
2019
Baylor University
2009-2018
Division of Undergraduate Education
2018
Texas A&M Health Science Center
2006-2016
Hyperbaric oxygen (HBO) is being studied as a therapeutic intervention for ischemia/reperfusion (I/R) injury. We have developed an in vitro endothelial cell model of I/R injury to study the impact HBO on expression intercellular adhesion molecule-1 (ICAM-1) and polymorphonuclear leukocyte (PMN) adhesion. Human umbilical vein (HUVEC) bovine aortic (BAEC) induction ICAM-1 required simultaneous exposure both hypoxia hypoglycemia determined by confocal laser scanning microscopy, ELISA, Western...
Chondrocytes in specific areas of the chick sternum have different developmental fates. Cephalic chondrocytes become hypertrophic and secrete type X collagen into extracellular matrix prior to bone deposition. Middle caudal remain cartilaginous throughout development continue types II, IX, XI. The interaction integrin receptors with molecules has been shown affect cytoskeleton organization, proliferation, differentiation, gene expression other cell types. We hypothesized that chondrocyte...
Periodontal mesenchymal stem cells (MSCs) play a crucial role in maintaining periodontium homeostasis and tissue repair. However, little is known about how periodontal MSCs vivo respond under disease conditions, posing challenge for regeneration. In this study, Gli1 was used as MSC marker combined with Gli1-cre ERT2 mouse model lineage tracing to investigate fate an induced periodontitis model. Our findings show significant changes the number contribution of + within inflamed periodontium....
We have analyzed embryonic chicken cornea for the presence of type IX collagen mRNA and protein. Using RNA transfer blot analysis, we demonstrate that alpha 1(IX) 2(IX) mRNAs are expressed by corneal epithelial cells at time primary stromal components synthesized. The levels decrease with increasing developmental age barely detectable day 11 development. In contrast, protein is immunofluorescence days 5 6 undetectable 8. probes specific mRNAs, size same in as chondrocytes, major source...
ABSTRACT Objectives: To study effects of various concentrations hydrogen peroxide on mature waterline biofilms and in controlling planktonic (free-floating) organisms simulated dental treatment water systems; to vitro the 2%, 3%, 7% removal inorganic compounds waterlines. Methods: Four units an automated unit system simulation device was used for 12 weeks. All were initially cleaned control deposits. H2O2 at 1%, 3% weekly periodic cleaning three group (units 1, 2 & 3), with 0.05%, 0.15%...
ABSTRACT The optic vesicle develops as an evagination of the cephalic neural folds. We have examined early development in Swiss Webster mice using correlated transmission electron microscopy (TEM), scanning (SEM), light microscopic (LM) measurements cell shape changes, immunohistochemical localization basal lamina (BL) components (type IV collagen, laminin and heparan sulphate proteoglycan (HSPG)) ultrastructural analysis BL. Like neuroepithelium other regions, low columnar cells plate...
The goals of this study were (1) to compare the injury at basement membrane zone (BMZ) rabbit corneal organ cultures exposed half mustard (2 chloroethyl ethyl sulfide, CEES) and nitrogen with that in vivo eyes sulfur (SM); (2) test efficacy 4 tetracycline derivatives attenuating vesicant-induced BMZ disruption 24-h period postexposure; (3) use most effective derivative improvement when drug is delivered as drops or hydrogels SM.Histological analysis hematoxylin eosin–stained sections was...
Abstract In palatogenesis, the MEE (Medial Edge Epithelium) cells disappear when palates fuse. We hypothesize that undergo EMT (Epithelial‐Mesenchymal Transition) to achieve mesenchyme confluence. Twist has an important role in for tumor metastasis. The purpose of this study was analyze function during palatal fusion. protein expressed shelves and both vivo vitro just prior mRNA increased chicken 3 6 hr after TGFβ3 treatment. Palatal fusion decreased cultured were treated with 200 nM siRNA...
The development of the head involves interaction several cell populations and coordination signalling pathways, which when disrupted can cause defects such as facial clefts. This review concentrates on genetic contributions to clefts with without cleft palate (CP). An overview early palatal emphasis muscle bone is blended effects environmental insults known mutations that impact human development. extensive table genes in syndromic non-syndromic CP, or lip (CL), provided. We have also...
Cigarette smoking is a risk factor in the development of periodontal diseases. In addition, delayed healing process has been shown smokers compared with non-smokers after treatment. Cell migration key wound and it highly regulated by variety signalling pathways. The small G protein, Rac, necessary for cell migration. Our aim was to determine if nicotine disrupted Rac its downstream proteins, p21-activated kinase 1/2 (PAK1/2), p44/42 mitogen-activated protein (MAPK) (extracellular...
Abstract Cigarette smoking has been suggested as a risk factor for several periodontal diseases. It also found that smokers respond less favorably than non‐smokers to therapy. Previous work in our lab shown nicotine inhibits human gingival cell migration. Since myofibroblasts play an important role wound closure, we asked if affects healing process by regulating myofibroblast differentiation. Human fibroblasts (HGFs) from two patients were cultured 10% fetal bovine serum culture medium....
Multiple studies have shown that dentin matrix protein 1 (DMP1) is essential for bone and mineralization. After post-translational proteolytic cleavage, DMP1 exists within the extracellular of as an NH 2 -terminal fragment, a COOH-terminal proteoglycan form 2- terminal fragment (DMP1-PG). To begin to assess biological function each we evaluated distribution both fragments in rat tooth using antibodies specific regions confocal microscopy. In first molar organs, localized predentin, whereas...
Dentin matrix protein 1 (DMP1) has been shown to be critical for the formation of dentin and bone. However, precise pathway by which DMP1 participates in dentinogenesis osteogenesis remains clarified. is present extracellular bone as processed NH<sub>2</sub>- COOH-terminal fragments. The NH<sub>2</sub>-terminal fragment occurs a proteoglycan, whereas highly phosphorylated. differences biochemical properties suggest that these fragments may have different tissue cell...
Background: Smoking is associated with an increased risk of oral health and dental problems. The aim this study to address the hypothesis that nicotine impairs wound healing by increasing reactive oxygen species inhibiting cell migration, antioxidants (AOs) may counteract effects. Methods: Primary human gingival fibroblasts (HGFs) periodontal ligament (HPDL) were grown confluence, pretreated 6 mM for 2 hours, treated AOs in presence nicotine. pure AO compounds ferulic acid (F), phloretin...
Abstract The development of suitable scaffolds for bone tissue engineering requires an in‐depth understanding the interactions between osteoblasts and scaffolding biomaterials. Although there have been a large amount knowledge accumulated on cell–material two‐dimensional (2D) planar substrates, our how respond to biomimetic nanostructured three‐dimensional (3D) scaffold is very limited. In this work, we developed approach use confocal microscopy as effective tool visualizing, analyzing,...