A5065387771- Hepatitis C virus research
- Hepatitis Viruses Studies and Epidemiology
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Animal Virus Infections Studies
- Veterinary medicine and infectious diseases
- Viral Infections and Immunology Research
- Liver Diseases and Immunity
- Cardiac Valve Diseases and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- Oral microbiology and periodontitis research
- Plant Virus Research Studies
- Virus-based gene therapy research
- Periodontal Regeneration and Treatments
- Cardiac Structural Anomalies and Repair
- Cardiac Imaging and Diagnostics
- Cardiac and Coronary Surgery Techniques
- Marine and fisheries research
- Cardiovascular Function and Risk Factors
- Coronary Interventions and Diagnostics
- Aortic Disease and Treatment Approaches
- Bacteriophages and microbial interactions
Jichi Medical University
2016-2025
Yokohama Municipal Citizen's Hospital
2023-2024
St. Luke's International Hospital
2019-2024
Toyama Prefectural University
2018-2024
Aishin Memorial Hospital
2024
Kawasaki Medical School
2015-2023
Takamatsu Red Cross Hospital
2015-2023
Hyogo Prefectural Awaji Medical Center
2011-2022
Center for Global Health
2022
National Center for Global Health and Medicine
2022
Based on variation in nucleotide sequence within restricted regions the putative C (core) gene of hepatitis virus (HCV), four groups HCV have been postulated a panel 44 isolates. They were provisionally designated types I, II, III and IV. A method for typing was developed, depending amplification by polymerase chain reaction using universal primer (sense) mixture type-specific primers (antisense). determined size products specific to each them. Type II found samples from 131 (82%) 159 blood...
Summary The complete nucleotide sequences of the DNA three hepatitis B virus (HBV) genomes subtype adw, cloned from plasma samples asymptomatic carriers living in mainland and Okinawa Prefecture Japan Indonesia were determined. All comprised 3215 bp differed sequence by only 3.9 to 5.6%. When these isolates compared with reported two HBV same derived American carriers, however, differences greater (8.3 9.3%) an extent comparable divergence between genome adw that a heterotypic subtype, such...
Although there are case reports of vertical transmission hepatitis C virus (HCV), it remains uncertain to what extent infected mothers transmit this their infants.We investigated the HCV from babies by analyzing RNA in blood. Three independent studies were performed. First, 7698 parturient women tested for anti-HCV antibodies; 53 positive. Their 54 infants (including one set twins) followed prospectively at least six months and disease studied. Third, families three HCV-infected examined...
The family Hepeviridae consists of positive-stranded RNA viruses that infect a wide range mammalian species, as well chickens and trout. A subset these infects humans can cause self-limiting acute hepatitis may become chronic in immunosuppressed individuals. Current published descriptions the taxonomical divisions within are contradictory relation to assignment species genotypes. Through analysis existing sequence information, we propose taxonomic scheme which is divided into genera...
Among ten patients who contracted sporadic acute or fulminant hepatitis E between 2001 and 2002 in Hokkaido, Japan, nine (90 %) had a history of consuming grilled undercooked pig liver 2–8 weeks before the disease onset. We tested packages raw sold grocery stores as food Hokkaido for presence virus (HEV) RNA by RT-PCR. Pig specimens from seven (1·9 363 detectable HEV RNA. Partial sequence analyses revealed that swine isolates belonged to genotype III IV. One isolate (swJL145) packaged 100 %...
The genomic DNA of a novel virus named TT (TTV), associated with posttransfusion hepatitis unknown etiology, was cloned from plasma blood donor an elevated transaminase level but without serological markers known viruses, and its sequence 3739 bases determined. TTV had density 1.26 g/cm3 in sucrose, which did not change after the treatment Tween 80. viral genome sensitive to DNase I Mung Bean Nuclease. Hence, would be unenveloped, single-stranded virus. Two possible open reading frames...
The C gene of hepatitis B virus (HBV) codes for a nucleocapsid protein made 183 amino acid residues and is preceded in phase by the precore (pre-C) region, encoding 29 residues. pre-C-region product required synthesis secretion e antigen (HBeAg), which C-terminal 10 N-terminal 149 C-gene product. HBV mutants with defects prevailed circulation three asymptomatic carriers as they seroconverted from HBeAg to corresponding antibody (anti-HBe), these finally replaced nondefective HBV. DNA clones...
Hepatitis B virus (HBV) DNA clones were propagated from 57 carriers with antibody to hepatitis e antigen (HBeAg) and sequenced within nucleotides (nt) 1685 1926 including the core promoter (nt 1742 1849) pre-C region 1814 1900). Mutations in or those region, both, detected 328 (97.9%) of 335 them. Five infected HBV mutants mutations alone, while 20 only abort translation HBeAg precursor; remaining 32 both region. Some exclusively had high titers, comparable wild-type HBV, thereby indicating...
The complete nucleotide sequence of a hepatitis C virus derived from plasma human carrier in Japan was determined. cDNA the isolate (HC-J6) contained 9481 nucleotides and an additional T stretch 30 to 108 at 3′ end, had one large open reading frame coding for polyprotein 3033 amino acids. It differed by 31.8 32.1% 27.4 27.7% acid American two Japanese isolates previously reported. Among these four isolates, 5′ non-coding region 329 341 well conserved (>93% identity), whereas 39 45 (T...
A recently discovered non-A–E hepatitis virus has been designated GB C (HGBV-C), but little is known about its mode of transmission and clinical manifestations. We studied 519 patients on maintenance hemodialysis to determine whether they were infected with HGBV-C.
Hepatitis C virus infections in medical personnel after needlestick accidents have been documented generally by detection of seroconversion to a hepatitis nonstructural region antigen, c100-3 (a marker infection). We tested for core-derived antibodies and genomic RNA addition antibody 159 cases exposure that did not involve patients positive HBsAg. Of these we found 68 with index both members negative HCV core or before the accidents. Seven became infected Their was subclinical self-limited...
We have identified four new hepatitis C virus (HCV) isolates whose genomic RNA could be amplified by PCR using primers from the 5' untranslated region (UTR), but not detected with genotype I to IV (or types 1a, 1b, 2a and 2b respectively)-specific core region-derived primers. compared nucleotide sequences of positions 65 1850 (3' end UTR, C, E1 E2/NS1) 8276 9394 NS5 3' UTR) those for genotypes IV. The had following characteristics: (i) overall sequence similarity between was 95 96%, 73 74%,...
The nomenclature of hepatitis E virus (HEV) subtypes is inconsistent and makes comparison different studies problematic. We have provided a table proposed complete genome reference sequences for each subtype. criteria subtype assignment vary between genotypes methodologies, so conservative pragmatic approach has been favoured. Updates to this will be posted on the International Committee Taxonomy Viruses website (http://talk.ictvonline.org/r.ashx?C). use common facilitate communication...
In this recommendation, we update our 2016 table of reference sequences subtypes hepatitis E virus (HEV; species Orthohepevirus A , family Hepeviridae ) for which complete genome are available (Smith et al ., 2016). This takes into account subsequent publications describing novel viruses and additional proposals subtype names; there now eight genotypes 36 subtypes. Although it remains difficult to define strict criteria distinguishing between subtypes, is not within the remit International...
We recently developed a cell culture system for hepatitis E virus (HEV) in PLC/PRF/5 and A549 cells, using fecal specimens from HEV-infected patients. Since transfusion-associated has been reported, we examined cells the ability to support replication of HEV various serum samples obtained 23 patients with genotype 1, 3, or 4 HEV. progenies emerged media regardless coexistence antibodies but dependent on load inoculated (31% at 2.0 x 10(4) copies per well 100% >or=3.5 well), were successfully...
The family Hepeviridae includes enterically transmitted small non-enveloped positive-sense RNA viruses. It the genera Piscihepevirus, whose members infect fish, and Orthohepevirus, mammals birds. Members of genus Orthohepevirus include hepatitis E virus, which is responsible for self-limiting acute in humans several mammalian species; infection may become chronic immunocompromised individuals. Extrahepatic manifestations Guillain-Barré syndrome, neuralgic amyotrophy, glomerulonephritis...
The family Hepeviridae includes enterically transmitted small quasi-enveloped or non-enveloped positive-sense single-stranded RNA viruses infecting mammals and birds (subfamily Orthohepevirinae) fish (Parahepevirinae). Hepatitis E virus (genus Paslahepevirus) is responsible for self-limiting acute hepatitis in humans; the infection may become chronic immunocompromised individuals extrahepatic manifestations have been described. Avian Avihepevirus) causes hepatitis-splenomegaly syndrome...