The cyclooxygenase (COX) product, prostacyclin (PGI 2 ), inhibits platelet activation and vascular smooth-muscle cell migration proliferation. Biochemically selective inhibition of COX-2 reduces PGI biosynthesis substantially in humans. Because deletion the receptor accelerates atherogenesis fat-fed low density lipoprotein knockout mouse, we wished to determine whether would accelerate this model. To address hypothesis, used dosing with nimesulide, which inhibited ex vivo , depressed urinary...

Endothelial dysfunction and excessively stimulated autophagy, often caused by oxidant injury or inflammation, will lead to atherosclerosis development progression in diabetes.The aim of this study is investigate the protective effect glucagon-like peptide-1 (GLP-1) treatment on preventing oxidative stress-induced endothelial autophagy.Treatment cells with GLP-1 significantly attenuated which was associated reduction intracellular reactive oxygen species (ROS) levels.These effects were likely...

Objective: Apoptosis and autophagy of retinal cells, which may be induced by oxidative stress, are tightly associated with the pathogenesis diabetic retinopathy (DR). The stress is considered as excessively stimulated autophagy, accelerates progression DR. This study aims to investigate protective effect GLP-1 treatment on alleviating apoptosis cells in type 2 rats reveals its possible mechanism. Methods: Type were fed high sugar, fat diet followed streptozotocin injection. was applied treat...

The mechanisms underlying fibrogenic responses after injury are not well understood. Epithelial cell cycle arrest in G2/M is a key checkpoint for determining wound-healing leading to either normal proliferation or fibrosis. Here, we identify kidney- and liver-enriched circular RNA, circBNC2, which abundantly expressed renal tubular cells hepatocytes but significantly downregulated acute ischemic toxic insult. Loss of circBNC2 at least partially mediated by upregulation DHX9. Gain-...

We hypothesized that moderate cardiac-selective overexpression of the angiotensin II type 2 receptor (AT2R) would protect myocardium from ischaemic injury after a myocardial infarction (MI) induced by coronary artery ligation. For in vitro studies, adenoviral vector expressing genomic DNA AT2R and enhanced green fluorescence protein (EGFP) was used to overexpress rat neonatal cardiac myocytes. Expression AT2R, measured real-time PCR immunostaining, demonstrated efficient transduction...

Objective. To determine plasma markers of oxidative stress during the second and third trimester pregnancy in patients with gestational diabetes mellitus (GDM). Study Design. We conducted a prospective nested case-control study involving 400 pregnant women, 22 whom developed GDM. As control group, 30 normal women were chosen randomly. Plasma samples analyzed for 8-iso-prostaglandin F2 α (8-iso-PGF2 ), advanced protein products (AOPPs), carbonyl (PCO), glutathione peroxidase-3 (GPX-3),...

Angiotensin II (Ang II) elicits Ang type 1 receptor (AT1-R)-mediated increases in neuronal firing within the hypothalamus and brainstem that are ultimately responsible for physiological actions such as increased blood pressure fluid intake. Although there is a growing literature on intracellular mechanisms mediate of via AT1-R neurons, little known about diminish or “switch-off” chronotropic action II. In present study, we identified macrophage migration inhibitory factor (MIF) an inhibitor...

The aim of the present study was to compare effects adipose‑derived mesenchymal stem cell (ADSC) and bone marrow (BMSC) transplantation into corpora cavernosa diabetic rats with erectile function. ADSCs BMSCs were isolated identified by flow cytometry. Rats streptozocin‑induced diabetes screened using apomorphine obtain a rat model dysfunction, followed cavernosa. Two weeks later, again injected apomorphine, intracavernous pressure (ICP) mean arterial (MAP) penile tissue measured, corpus...

Ureteral stricture caused by holmium: YAG laser lithotripsy is one of the most challenging issues for urologists. Currently, evidence rapamycin application in reducing ureterostenosis not sufficient. This study aimed to assess inhibition ureteral rapamycin-eluting stents vitro and vivo. A bilayered drug-eluting stent consisted drug blending with poly (lactic-co-glycolic acid) (PU/drug stent), which was over-layered polycaprolactone (PCL) ultrasonic atomizing spraying. Stent morphology...

Bladder cancer is a common tumor type of the urinary system, which has high levels morbidity and mortality. The first‑line treatment cisplatin‑based combination chemotherapy, but significant proportion patients relapse due to development drug resistance. Therapy‑induced senescence can act as 'back‑up' response chemotherapy in types that are resistant apoptosis‑based anticancer therapies. circadian clock serves an important role resistance cellular senescence. aim present study was...

Gestational diabetes mellitus (GDM) is one of the most common complications in pregnancy, impairing both maternal and fetal health short long term. As early interventions are considered desirable to prevent GDM, this study aims develop a simple-to-use nomogram based on multiple risk factors from electronic medical records (EMHRs). A total 924 pregnant women whose EMHRs were available at Peking University International Hospital January 2022 October included. Clinical demographics routine...

AimsThe macrophage migration inhibitory factor (MIF) is an intracellular inhibitor of the central nervous system actions angiotensin II on blood pressure. Considering that at nucleus solitary tract are important for maintenance hypertension in spontaneously hypertensive rats (SHRs), we tested if increased MIF expression SHR alters baseline high pressure these rats.

Abstract BACKGROUND Macrophage migration inhibitory factor (MIF) is an intracellular regulator of the actions angiotensin II in central nervous system. Renovascular hypertensive 2-kidney, 1-clip (2K1C) rats have increased activity renin–angiotensin system and a decrease baroreflex function compared to normotensive (NT) rats. In present study, we tested effects MIF overexpression within nucleus solitary tract (NTS), key brainstem region for cardiovascular regulation, on development...

BACKGROUND:Glucagon-like peptide-1 (GLP-1) has been reported to exert some beneficial effects on the central nervous system (CNS). However, effect of GLP-1 cognitive impairment associated with type 2 diabetes is not well known. This study investigated ameliorating memory deficits in diabetic rats. MATERIAL AND METHODS:Type rats were induced by a high-sugar, high-fat diet, followed streptozotocin (STZ) injection and then tested Morris Water Maze (MWM) 1 week after induction diabetes. The mRNA...

Polyurethane (PU) ureteral stents are used in clinics to maintain the patency. Due biofilm formation and encrustation complications, long-term clinical usage has been limited. It is therefore necessary develop an effective response this unmet medical need. A heparin/poly-l-lysine/copper (NPs) coating was developed our previous work that showed effect of preventing infection vitro. In work, a further study conducted by grafting NPs on then were implanted into infectious bladders Wistar rats...

Increasing MIF levels in the NTS reduces blood pressure SHR. Since inflammation has an important role hypertension we investigated whether increasing of SHR alters inflammatory cytokine and microglial activation. Male normotensive (NT) rats (8 weeks old) were microinjected with either AAV2‐CBA‐MIF or AAV2‐CBA‐eGFP to elicit chronic increases GFP (control) expression. Thirty days later, analyzed mRNAs activation NTS. Baseline pro‐inflammatory (PIC) such as IL‐1β, IL‐6 TNFα significantly...

Abstract Background: Wilson’s disease (WD) is a rare autosomal recessive inherited disorder of chronic copper toxicosis with high mortality and disability. Recent evidence suggests correlation between dysbiosis in the gut microbiome metabolic disease. Therefore, impact intestinal microbiota polymorphism WD need to be explore for seeking some benefit patients. Methods: In this study, 16S rRNA sequencing was performed on fecal samples from 14 patients were compared results 16 healthy...

Introduction: Ureteral stents blocked with encrustation are a common clinical complication and affect bacteria colonization inflammatory response. In this study, different concentrations of copper (0.25, 0.5, 1, 1.5, 2 g/L) were immobilized on polyurethane (PU) that showed functionalization microbe resistance regulation the inflammation response to RAW264.7. Methods: X-ray photoelectron spectroscopy (XPS), atomic force microscope (AFM) static water contact angles used analyze surface...

We previously reported that chronic increases in corticosterone within the dorsal hindbrain, including Nuclues of Solitary Tract (NTS), augment arterial pressure response to restraint stress. This study tested hypothesis diminishing actions selectively NTS catecholaminergic neurons would attenuate cardiovascular responses novel and repeated AAV2-based viral vectors were constructed express either GFP or 11-beta-hydroxysteroid dehydrogenase 2 (HSD2) using PRSx8 promoter. HSD2 degrades an...

The PVN is crucial to the CNS regulation of sympathetic outflow. Glutamate prevalent excitatory neurotransmitter in CNS, including PVN. Oxytocin (OT) specifically produced by and supraoptic nucleus neurons, microinjection OT into RVLM induces a pressor response. current study aimed at determining whether glutamate neurons projecting contain as co‐transmitter. Real time RT‐PCR analyses tissues from 4‐6 week‐old Sprague‐Dawley (SD) rats revealed presence phosphate‐activated glutaminase (PAG;...

We have shown that increases in dorsal hindbrain (DHB) corticosterone (Cort) enhance the blood pressure response to acute stress. Stress activates brain angiotensin type I receptors (AT1R), and glucocorticoids can increase AT1R expression. Thus, we hypothesized chronic DHB Cort elevate expression binding within nucleus of solitary tract (NTS). Male Sprague‐Dawley rats were treated with sham, 50% or 100% pellets, pellets subcutaneously (sc) for 6 days. mRNA was analyzed by quantitative...