A5062437071- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Molecular Junctions and Nanostructures
- Electrochemical Analysis and Applications
- RNA and protein synthesis mechanisms
- Electrochemical sensors and biosensors
- Conducting polymers and applications
- Analytical Chemistry and Sensors
- Molecular Sensors and Ion Detection
- X-ray Diffraction in Crystallography
- Free Radicals and Antioxidants
- Nanofabrication and Lithography Techniques
- nanoparticles nucleation surface interactions
- Photochromic and Fluorescence Chemistry
- Crystallization and Solubility Studies
- Crystallography and molecular interactions
- Acoustic Wave Resonator Technologies
- Click Chemistry and Applications
- RNA Research and Splicing
- Biochemical effects in animals
- Electrocatalysts for Energy Conversion
- Spectroscopy Techniques in Biomedical and Chemical Research
- Genomics and Chromatin Dynamics
Inserm
2013-2023
Université de Bordeaux
2014-2023
Institut Européen de Chimie et Biologie
2013-2023
Centre National de la Recherche Scientifique
2016-2023
University of Namur
2022-2023
Régulations Naturelle et Artificielle
2014-2022
Canadian Nautical Research Society
2019
Université Libre de Bruxelles
2009-2015
The G-quadruplexes (G4s) are currently being explored as therapeutic targets in cancer and other pathologies. Six carbohydrate naphthalene diimide conjugates (carb-NDIs) have been synthesized G4 ligands to investigate their potential selectivity binding cell penetration. Carb-NDIs shown certain for structures against DNA duplexes, but different sugar moieties do not induce a preference specific topology. Interestingly, when monosaccharides were attached through short ethylene linker the NDI...
A series of nine Ni(II) salophen complexes involving one, two, or three alkyl-imidazolium side-chains was prepared. The lengths the were varied from one to carbons. crystal structure complex revealed a square planar geometry nickel ion. Fluorescence resonance energy transfer melting G-quadruplex structures in presence performed. DNA stabilized complexes, but duplex not. binding constants for parallel and antiparallel DNA, as well hairpin measured by surface plasmon resonance. compounds...
A bulky cationic cobalt(<sc>iii</sc>) porphyrin carrying two water molecules as axial ligands interacts in a π-stacking like mode with the 5′-external guanine quartet of G-quadruplex DNA.
G-quadruplexes (G4s) are four-stranded nucleic acid structures formed by the stacking of G-tetrads. Here we investigated their formation and function during HIV-1 infection. Using bioinformatics biophysics analyses first searched for evolutionary conserved G4-forming sequences in genome. We identified 10 G4s with conservation rates higher than those regulatory such as RRE TAR. then used porphyrin-based G4-binders to probe infection human cells native HIV-1. The efficiently inhibited...
Abstract Analytical performance (selectivity, sensitivity, limit of detection) a sensor is ultimately connected to the interfacial architecture sensing surface that needs be optimized. Self‐assembly mixed monolayer thiolated probes and diluent onto gold surfaces used in design biosensing platforms nowadays commonly performed by two‐step immobilization procedure. In this work, merits one‐step coadsorption procedure are emphasized, it shown ionic strength very efficient tool fine control...
Abstract Aptamer‐based biosensors offer promising perspectives for high performance, specific detection of proteins. The thrombin binding aptamer (TBA) is a G‐quadruplex‐forming DNA sequence, which frequently elongated at one end to increase its analytical performances in biosensor configuration. Herein, we investigate how the elongation TBA 5′ affects structure and stability. Circular dichroism spectroscopy shows that folds an antiparallel G‐quadruplex conformation with all studied cations...
G-quadruplex (G4) structures in intron 3 of the p53 pre-mRNA modulate 2 splicing, altering balance between fully spliced transcript (FSp53, encoding full-length p53) and an incompletely retaining (p53I2 N-terminally truncated Δ40p53 isoform). The nucleotides forming G4s overlap polymorphism rs17878362 (A1 wild-type allele, A2 16-base pair insertion) which is linkage disequilibrium with rs1642785 (c.74+38 G>C). Biophysical biochemical analyses show alleles form similar G4 as A1 although their...
Four nickel(II)-salophen complexes containing alkyl-imidazolium chains connected at the ortho or meta positions were prepared: N,N'-bis(2-hydroxy-4-methyl-3H-imidazol-1-iumbenzylideneamino)phenylenediamine (1), N,N'-bis(2-hydroxy-3-methyl-3H-imidazol-1-iumbenzylideneamino)phenylenediamine (2), N,N'-bis(2-hydroxy-3-methyl-3H-imidazol-1-iumbenzylideneamino)methyl-3H-imidazol-1-iumphenylenediamine (3), and...
The Caenorhabditis elegans model has greatly contributed to the understanding of role G-quadruplexes in genomic instability. GGCTTA repeats C. telomeres resemble GGGTTA human telomeres. However, comparison telomeric sequences (Homo sapiens, Tetrahymena, Oxytricha, Bombyx mori and Giardia) revealed that small changes these can drastically change topology folded G-quadruplex. In present work we determined structure adopted by sequence d[GG(CTTAGG)3]. investigated is shown fold into an...
Telomeres and telomerase are key players in tumorogenesis. Among the various strategies proposed for inhibition or telomere uncapping, stabilization of telomeric G-quadruplex (G4) structures is a very promising one. Additionally, G4 stabilizing ligands also act over tumors mediated by alternative elongation telomeres. Accordingly, discovery novel compounds able to on telomeres and/or inhibit enzyme DNA as well development approaches efficiently prioritizing such constitute active areas...
Analytical tools enabling the discrimination between duplex DNA and G-quadruplex are necessary to unravel biological function(s) of G-quadruplexes. A methodology relying on electrochemical response electroactive hexaammineruthenium(III) cation at DNA-modified surfaces is presented. characteristic voltammetric peak evidenced for all investigated sequences, encompassing various types folding numbers quartets. In contrast, no such detected dsDNA sequences. The occurrence consequence a strong...
Gold(<sc>iii</sc>) porphyrins have a square planar geometry and an extra positive charge, compatible with stronger electrostatic π-stacking interactions.
Abstract G‐quadruplexes formed by nucleic acids are implicated in pathologies ranging from cancers to neurodegenerative diseases. We evaluated interactions of 29 bi‐ and terpyridine derivatives with duplexes. FRET‐melting, circular dichroism, 1 H NMR spectroscopy showed that one derivative interacted very selectively G‐quadruplexes. This G‐quadruplex ligand inhibited helicase activity should influence G‐quadruplex‐related biological processes.
Abstract not Available.