A5054303171- Atherosclerosis and Cardiovascular Diseases
- Nail Diseases and Treatments
- Spinal Cord Injury Research
- Immune cells in cancer
- Liver Disease Diagnosis and Treatment
- Ginger and Zingiberaceae research
- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Nerve injury and regeneration
- Neurological Disease Mechanisms and Treatments
- interferon and immune responses
- Sirtuins and Resveratrol in Medicine
- Fungal Infections and Studies
- Bone health and osteoporosis research
- Drug-Induced Hepatotoxicity and Protection
- Cancer Genomics and Diagnostics
- Mitochondrial Function and Pathology
- Genomic variations and chromosomal abnormalities
- Neurogenesis and neuroplasticity mechanisms
- Inflammasome and immune disorders
- Intensive Care Unit Cognitive Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- American Constitutional Law and Politics
- Dermatology and Skin Diseases
Academy of Medical Sciences
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2025
Tongji University
2011-2025
Third Affiliated Hospital of Southern Medical University
2016-2025
Peking Union Medical College Hospital
2025
Jiangsu University
2025
Guangxi Medical University
2025
Shanghai Pulmonary Hospital
2013-2024
Guiyang Medical University
2016-2024
Key Laboratory of Guangdong Province
2023-2024
ABSTRACT In a large scale mutagenesis screen for embryonic mutants in zebrafish, we have identified 63 mutations 24 loci affecting the morphogenesis of zebrafish brain. The expression marker genes and integrity axonal scaffold been studied to investigate abnormalities regionalization, neurogenesis axonogenesis Mutants can be broadly classified into two groups, one regionalization along anterior-posterior or dorsal-ventral axis, other general features brain morphology. first group includes...
Previous attempts to promote regeneration after spinal cord injury have succeeded in stimulating axonal growth into or around lesion sites but rarely beyond them. We tested whether a combinatorial approach of the neuronal cell body with cAMP and injured axon neurotrophins would propel injury. A preconditioning stimulus sensory bodies was delivered by injecting L4 dorsal root ganglion, postinjury administered neurotrophin-3 (NT-3) within cervical site grafted autologous bone marrow stromal...
Acetaminophen (APAP) overdose is a leading cause of drug-induced liver injury (DILI). The impact the gut microbiota and associated metabolites on APAP function remains unclear. We show that disturbance with distinct microbial community, notable decreases in Lactobacillus vaginalis. Mice receiving L. vaginalis showed resistance to hepatotoxicity due liberation isoflavone daidzein from diet by bacterial β-galactosidase. hepatoprotective effects APAP-exposed germ-free mice were abolished...
The p53 tumor suppressor is a key mediator of the cellular response to stress. Phosphorylation induced by multiple stress-activated kinases has been proposed be essential for stabilization, interaction with transcriptional co-activators, and activation target genes. However, genetic studies suggest that phosphorylation may not activation. We therefore investigated role on six serine residues (Ser6, Ser15, Ser20, Ser37, Ser46, Ser392) using nutlin-3, recently developed small molecule MDM2...
Abstract Recent studies have shown that activation of cell cycle checkpoints can protect normal proliferating cells from mitotic inhibitors by preventing their entry into mitosis. These used genotoxic agents act, at least in part, the p53 pathway. However, drugs are known also to p53-independent activities and could affect sensitivity tumor antimitotic agents. Recently, we developed first potent selective small-molecule p53-MDM2 interaction, nutlins, which activate pathway only with...
In this study, We demonstrated that Bax mitochondrial translocation plays a vital role in the initiation of signaling pathway upon activation by heat stress. addition, both p53 and Ca(2+) signal mediated MPTP opening activate translocation. Employing pifithrin-α (a inhibitor) CsA permeability transition pore (MPTP) inhibitor), we found stress induced was significantly inhibited cells pretreated with PFT CsA. Furthermore, generation reactive oxygen species (ROS) is critical mediator apoptosis...
Abstract Background Adrenocortical carcinoma (ACC) is an extremely rare, aggressive tumor with few effective therapeutic options or drugs. Mitotane (Mtn), which the only authorized drug, came out in 1970 and still first-line treatment for ACC spite of serious adverse reaction a high recurrence rate. Methods By silico analysis dataset cancer genome atlas (TCGA), we determined that expression levels cyclin-dependent kinase-1 (CDK1) were significantly related to clinical outcomes ACC. In vitro...
Gastrodia elata Bl. has a long edible history and is considered an important functional food raw material. Gastrodin (GAS) one of the main substances in G. BI. can be used as health care product for elderly to enhance resistance delay aging. This study investigated ameliorative effect mechanism GAS on cognitive dysfunction vascular dementia (VaD) rats, which provides theoretical basis development utilization food. The water maze test shows that improves learning memory impairment VaD rats....
Abstract The corticospinal projection is considered to influence fine motor function through nearly exclusively contralateral projections from the cortex in primates. However, unilateral lesions this system various species are frequently followed by significant functional improvement, raising possibility that bilateral of pathway may exist or emerge after injury. To examine detailed anatomy and neurons rhesus monkeys (n = 4), we injected high‐resolution anterograde tracer biotinylated...
Neurogenesis has been described in various regions of the CNS throughout life. We examined extent natural cell division and replacement from 7 weeks to months after cervical spinal cord injury four adult rhesus monkeys. Bromodeoxyuridine (BrdU) injections revealed an increase >80-fold number newly divided cells primate injury, with average 725,000 BrdU-labeled identified per monkey immediate zone. By 15% these new expressed mature markers oligodendrocytes 12% astrocytic markers. Newly...
We hypothesized that the activation patterns of flexor and extensor muscles resulting kinematics forelimbs hindlimbs during locomotion in Rhesus would have unique characteristics relative to other quadrupedal mammals. Adaptations limb movements motor pool recruitment accommodating a range treadmill speeds similar terrestrial animals both hindlimb forelimb were observed. Flexor neurons from pools lumbar segments, however, more highly coordinated than cervical segments. Unlike lateral sequence...
Six adult monkeys (Macaca mulatta) received a unilateral lesion of the lateral corticospinal tract (CST) in thoracic spinal cord. Prior to surgery, animals were trained perform quadrupedal stepping on treadmill, and item retrieval with foot. Whole body kinematics electromyogram (EMG) recordings made prior to, at regular intervals over period 12 weeks after CST lesion. After 1 week recovery, all able walk unaided quadrupedally treadmill. The animals, however, dragged hindpaw ipsilateral along...
Olfactory ensheathing cells (OECs) have been reported to migrate long distances and bridge lesion sites, guiding axonal regeneration after spinal cord injury (SCI). To understand mechanisms of OEC migration guidance, we injected lamina propria OECs 1 mm rostral caudal C4 SCI sites. One month later, formed an apparent migrating cell tract continuously extending from the injection site through lesion, physically bridging lesion. Confocal immunolabeling demonstrated that, whereas this displaced...
Trace amine-associated receptor 1 (TAAR1) is a G protein-coupled that directly responds to endogenous monoamines as well amphetamine-related psychostimulants, including methamphetamine. In the present study, we demonstrate TAAR1 mRNA and protein expression in rhesus monkey brain regions associated with monoaminergic systems, variable cellular distribution of brain, coexpression dopamine transporter (DAT) subset neurons both mouse substantia nigra. On this basis, evaluated activation by...
Emodin [1,3,8-trihydroxy-6-methylanthraquinone] has been reported to exhibit vascular anti-inflammatory properties. However, the corresponding mechanisms are not well understood. The present study was designed explore molecular target(s) of emodin in modifying lipopolysaccharide (LPS)-associated signal transduction pathways endothelial cells.
Recently identified trace amine receptors are potential direct targets for drugs of abuse, including amphetamine and 3,4-methylenedioxymethamphetamine (MDMA). We cloned full-length rhesus monkey receptor 1 (rhTA(1)) that was 96% homologous to human TA(1). The amines tyramine beta-phenylethylamine (PEA) the monoamine transporter substrates (+/-)-amphetamine (+/-)-MDMA stimulated cAMP accumulation in rhTA(1)-expressing cell lines, as measured by a response element-luciferase assay. Cocaine did...
1,8-Cineole (eucalyptol), a monoterpene, has been widely reported for the anti-inflammatory effects. Our previous data confirmed that 1,8-cineole ameliorated inflammatory phenotype of human umbilical vein endothelial cells (HUVECs) by mediating NF-κB expression in vitro. At present, we investigated protection effects on vascular endothelium lipopolysaccharide (LPS)-induced acute injury mice and potential mechanisms involved HUVECs. Results from enzyme linked immunosorbent assays revealed...