Introduction: Osteoarthritis (OA) is the most prevalent joint disorder in elderly population, and inflammatory mediators like IL-1βwere thought to play central roles its development. Schisandrin B, main active component derived from Schisandra chinensis , exhibited antioxidative antiinflammatory properties. Methods: In present study, protective effect underlying mechanism of B on OA was investigated vivo vitro. Results: The results showed that decreased IL-1β-induced upregulation matrix...

Metabolic disorders and inflammation of chondrocytes are major pathological changes in aging cells osteoarthritis (OA). Recent studies demonstrated age-related mitochondrial dysfunction may be a key contributing factor the development OA. Mitofusin 2 (MFN2) is regulator fusion, cell metabolism, autophagy apoptosis. This study was performed to ascertain whether MFN2 involved OA.Metabolic measurements were taken rat between different ages (3-week, 5-month, 12-month). activity detected both...

Abstract Diabetes mellitus (DM) is one of the prominent risk factors for pathological development and progression tendinopathy. One feature DM‐related changes in tendinopathy accumulation advanced glycation end products (AGEs) affected tendons. Pioglitazone (Pio), a peroxisome proliferator‐activated receptor γ agonist, performs protective effect against AGEs. The present study aimed to investigate pathogenetic role AGEs on tendon‐derived stem cells (TDSCs) determine Pio AGEs‐induced TDSC...

NR4A3 is a member of nuclear receptor subfamily 4, which an important regulator cellular function and inflammation. In this study, high expression in human osteoarthritis (OA) cartilage was firstly observed. To explore the relationship between OA, we used lentivirus overexpression system to simulate its study role OA. Additionally, siRNA-mediated knockdown confirm findings experiments. The results showed stimulatory effect IL-1β on matrix-degrading enzyme such as MMP-3, 9, INOS COX-2...

Tendinopathy is a common musculoskeletal disorder, mainly affects the athletes and aged people. Tumor necrosis factor-α (TNF-α) plays an initiator role in tendinopathy. Tectorigenin extract component of Belam-canda Chinesis, which performs anti-inflammatory anti-apoptosis effects. The present study stablished to investigate tectorigenin against pathogenetic effects TNF-α on tendon-derived stem cells (TDSCs) vivo vitro. findings indicated that able induce TDSC inflammation, apoptosis...

Heterotopic ossification (HO) is a pathological condition involved in tendinopathy. Adipokines are known to play key role HO of Nesfatin-1, an 82-amino acid adipokine closely reportedly associated with diabetes mellitus (DM), which, turn, related In the present study, we aimed investigate effects nesfatin-1 on osteogenic differentiation tendon-derived stem cells (TDSCs) and pathogenesis tendinopathy rats. vitro , TDSCs were incubated induction medium for 14 days different concentration. vivo...

Competitive endogenous RNAs (ceRNAs), as a newly identified regulating mechanism, have been demonstrated to play crucial role in various human diseases. An increasing number of recent studies revealed that circular (circRNAs) can function ceRNAs. However, little is known about the circFAM160A2 pathological process osteoarthritis (OA). This study first examine circFAM160A2‐miR‐505‐3p‐SIRT3 axis progression. miR‐505‐3p was selected from interaction microRNA (miRNA) microarray comparing...

Osteoarthritis (OA) is one of the most common chronic musculoskeletal disorder worldwide, representing a major source disability, pain and socioeconomic burden. Yet effective pharmaceutical treatments applied in clinical works are merely symptomatic management with uncertainty around their long-term safety efficacy, namely no drugs currently capable modulating biological progression OA. Here, we identified potent anti-inflammatory as well anti-oxidative properties Nitidine Chloride (NitC),...

Osteoarthritis (OA) is a chronic degenerative joint disease characterized by deterioration of articular cartilage. Dual specificity phosphatase 5 (DUSP5), member the DUSP subfamily, known to regulate cellular inflammation. Here, we studied relationship between DUSP5 and OA knockdown overexpression DUSP5, respectively. Results from in vitro experiments demonstrated that increased interleukin-1β (IL-1β)-induced expression inflammatory genes, such as inducible nitric oxide synthase (iNOS),...

As a joint disease, osteoarthritis (OA) is caused by the breakdown of subchondral bone and cartilage damage. Inflammatory factors, such as interleukin- (IL-) 1 β , mediate progression OA. Madecassoside (MA), triterpenoid component derived from gotu kola herb ( Centella asiatica ), exhibits various pharmacological effects, including antioxidative anti-inflammatory properties. In present study, protective effects possible mechanism MA on treatment OA were investigated. was demonstrated to...

Tendinopathy is a disabling musculoskeletal disease, the pathological process of which tightly associated with inflammation. Spironolactone (SP) has been widely used as diuretic in clinical practice. Recently, SP shown anti-inflammatory features several diseases. Tendon-derived stem cells (TDSCs), subset cell type from tendon tissue possessing clonogenic capacity, play vital role tendinopathy. In present study, protective effect on TDSCs was demonstrated under simulated tendinopathy conditions both

Osteoarthritis (OA) is a chronic joint disease involving cartilage erosion and matrix degradation. Costunolide sesquiterpene lactone that has been demonstrated to exert anti‑inflammatory activities in wide variety of cells. The aim the present study was investigate effect costunolide OA treatment, using rat chondrocytes an model, which animals were subjected destabilization medial meniscus. results revealed (2‑6 µM) had no on chondrocyte viability or phenotype maintenance. decreased...

Abstract Autophagy and cytoskeleton integrity of chondrocytes are a considered as major factors in the progression osteoarthritis (OA) involving excessive chondrocyte apoptosis senescence. Nesfatin-1, an adipokine, has been reported to be closely related cell autophagy malfunction. Our previous study found that nesfatin-1 was highly correlated with OA progress patient, expression rises knee articular tissue, serum chondrocytes. In current study, we aimed explore therapeutic effect on its...

Abstract Background Osteoarthritis (OA) is a debilitating disease that inflicts intractable pain, major problem humanity faces, especially in aging populations. Stem cells have been used the treatment of many chronic diseases, including OA. Cartilage progenitor/stem (CPSCs) are type stem with ability to self- renew and differentiate. They hold promising future for understanding progression OA its treatment. Previous studies reported relationship between mitochondrial dynamics mesenchymal...

Abstract Metabolism of bone is regulated by the balance between osteoblast‐mediated formation and osteoclast‐mediated resorption. Activation osteoclasts could lead to osteoporosis. Thus, inhibiting activity becomes an available strategy for treatment Tectorigenin extract Belamcanda chinensis In present study, anti‐osteoclastogenesis effects tectorigenin were investigated in vitro vivo. The results showed preventive therapeutic at concentrations 0, 10, 40, 80 μmol/L maturation activation...

Osteosarcoma is a malignant bone tumor that prone to metastasize early and primarily affects children adolescents. Cell migration-inducing protein (CEMIP) plays crucial role in the progression malignancy of various diseases, including osteosarcoma. Chitosan oligosaccharide (COS), an oligomer isolated from chitin, has been found have significant anti-tumor activity cancers. This study investigates effects COS on CEMIP expression osteosarcoma explores underlying mechanism. In present study,...

Competitive endogenous RNAs (ceRNAs), as a newly identified regulating mechanism, have been demonstrated to play crucial role in various human diseases. An increasing number of recent studies revealed that circular (circRNAs) can function ceRNAs. However, little is known about the circFAM160A2 pathological process osteoarthritis (OA). This study first examine circFAM160A2-miR-505-3p-SIRT3 axis progression. miR-505-3p was selected from interaction microRNA (miRNA) microarray comparing...

Abstract Osteosarcoma is a malignant bone tumor that prone to metastasize early and primarily affects children adolescents. Cell migration-inducing protein (CEMIP) plays crucial role in the progression malignancy of various diseases, including osteosarcoma. Chitosan oligosaccharide (COS), an oligomer isolated from chitin, has been found have significant anti-tumor activity cancers. This study investigates effects COS on CEMIP expression osteosarcoma explores underlying mechanism. In present...

Abstract Autophagy and cytoskeleton integrity of chondrocytes are a considered as major factors in the progression osteoarthritis (OA) involving excessive chondrocyte apoptosis senescence. Nesfatin-1, an adipokine has been reported to be closely related cell autophagy malfunction. Our previous study found that nesfatin-1 was highly correlated with OA progress patient, expression rises knee articular tissue, serum chondrocytes. In current study, we aimed explore therapeutic effect on its...

Abstract Background: Osteoarthritis (OA) is a debilitating disease that inflicts intractable pain, major problem humanity faces, especially in aging populations. Stem cells have been used the treatment of many chronic diseases, including OA. Cartilage progenitor (CPCs) are type stem with ability to self- renew and differentiate. They hold promising future for understanding progression OA its treatment. Previous studies reported relationship between mitochondrial dynamics mesenchymal cell...