A5041107220- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Adipokines, Inflammation, and Metabolic Diseases
- Genetics and Physical Performance
- Diet and metabolism studies
- Exercise and Physiological Responses
- Obstructive Sleep Apnea Research
- Nutrition and Health in Aging
- Cancer-related gene regulation
- Muscle Physiology and Disorders
- Pharmacology and Obesity Treatment
- Body Contouring and Surgery
- Therapeutic Uses of Natural Elements
- Liver Disease Diagnosis and Treatment
- Nuclear Structure and Function
- Immune Cell Function and Interaction
- Bariatric Surgery and Outcomes
- Nutritional Studies and Diet
- Sirtuins and Resveratrol in Medicine
- Childhood Cancer Survivors' Quality of Life
- Glycogen Storage Diseases and Myoclonus
- Restless Legs Syndrome Research
- Telomeres, Telomerase, and Senescence
- COVID-19 and Mental Health
- Heat shock proteins research
Inserm
2012-2024
Université Grenoble Alpes
2024
Santé Publique France
2018-2022
Novo Nordisk Foundation
2016-2021
University of Copenhagen
2016-2021
Physiologie et Médecine Expérimentale du Coeur et des Muscles
2012-2018
Centre National de la Recherche Scientifique
2016-2018
Université de Montpellier
2012-2018
State of the art techniques have been developed to isolate and analyze cells from various tissues, aiming capture their in vivo state. However, majority cell isolation protocols involve lengthy mechanical enzymatic dissociation steps followed by flow cytometry, exposing stress disrupting physiological niche. Focusing on adult skeletal muscle stem cells, we a protocol that circumvents impact procedures captures native quiescent We show current induce major transcriptional changes accompanied...
Methylation of nuclear genes encoding mitochondrial proteins participates in the regulation mitochondria function. The existence cytosine methylation genome is debated. To investigate whether DNA (mtDNA) methylated, we used both targeted- and whole bisulfite sequencing cell lines muscle tissue from mouse human origin. While unconverted cytosines were detected some portion genome, their abundance was inversely associated to depth, indicating that analysis can bias estimation mtDNA levels. In...
Remodeling of the sperm epigenome by lifestyle factors before conception could account for altered metabolism in next generation offspring. Here, we hypothesized that endurance training changes human spermatozoa. Using small RNA (sRNA) sequencing and reduced representation bisulfite (RRBS), we, respectively, investigated sRNA expression DNA methylation pure fractions motile spermatozoa collected from young healthy individuals before, after 6 weeks 3 months without exercise. Expression 8 PIWI...
Aim: To determine the genomic mechanisms by which adipose tissue responds to acute and chronic exercise. Methods: We profiled transcriptomic epigenetic response exercise in human collected before after endurance training. Results: Although exercises were performed at same relative intensities, magnitude of changes was reduced DNA methylation remodeling induced more prominent trained versus untrained state. found an overlap between gene expression for 32 genes pre-training six post-training,...
Exposure to ionizing radiation increases the risk of chronic metabolic disorders such as insulin resistance and type 2 diabetes later in life. We hypothesized that irradiation reprograms epigenome progenitor cells, which could account for impaired metabolism after cancer treatment. C57Bl/6 mice were treated with a single dose subjected high-fat diet (HFD). RNA sequencing reduced representation bisulfite used create transcriptomic epigenomic profiles preadipocytes skeletal muscle satellite...
Cancer treatments have substantially improved childhood cancer survival but are accompanied by long-term complications, notably chronic inflammatory diseases. We hypothesize that could lead to epigenetic changes in immune cells, resulting increased prevalence of diseases survivors. To test this hypothesis, we established the and transcriptomic profiles cells from 44 survivors (CCS, > 16 years old) on full remission (> 5 years) who had received chemotherapy alone or combination with total...
Exercise training triggers numerous positive adaptations through the regulation of genes controlling muscle structure and function. Epigenetic modifications, including DNA methylation, participate in transcriptional activation by allowing recruitment transcription machinery to gene promoters. induces dynamic demethylation at promoters; however, contribution precursor hydroxymethylcytosine is unknown. Given evanescent nature hydroxymethylcytosine, a contraction model that allows for...
Context Obesity is associated with insulin-resistance (IR), the key feature of type 2 diabetes. Although chronic low-grade inflammation has been identified as a central effector IR development, it never investigated simultaneously at systemic level and locally in skeletal muscle adipose tissue obese humans characterized for their insulin sensitivity. Objectives We compared metabolic parameters levels normal-weight subjects different sensitivity to better understand mechanisms involved...
Separately, polyphenols and exercise are known to prevent insulin resistance (IR) but their combined curative effects on established obesity IR require further investigation. Therefore, we compared the metabolic of a combination grape supplementation in obese rats with high-fat diet (EXOPP) effect alone (HF) or nutritional (PP) endurance (EXO) during 8 wks. We observed an improvement systemic skeletal muscle sensitivity EXO EXOPP rats. HF presented lower insulinemia HOMA-IR higher liver...
Obesity is associated with chronic low-grade inflammation and oxidative stress that blunt insulin response in its target tissues, leading to resistance (IR). IR a characteristic feature of type 2 diabetes. Skeletal muscle responsible for 75% total insulin-dependent glucose uptake; consequently, skeletal considered be the primary defect systemic development. Interestingly, some obese people stay insulin-sensitive metabolically healthy. With aim understanding this difference identifying...
Dietary intervention is a cornerstone of weight loss therapies. In obesity, dysbiotic gut microbiota (GM) characterized by high levels Bacteroides lineages and low diversity. We examined the GM composition changes, including 2 enterotype (Bact2), in real-world study subjects following high-protein hypocaloric diet with or without live microorganisms (LMP) supplement.263 volunteers were part this program. The first phase was low-carbohydrate calorie restriction LMP supplements. Fecal samples...
The aim of this study was to assess the impact nationwide total lockdown (LD) in France on weight loss and body composition modifications subjects participating a program evaluate remote consultations participants' adherence program. CO-RNPC prospective multicentre cohort including participants undergoing two six months rate kg/week computed before (15 days), during (99 days) after LD days). In 1550 completing participants, decreased from 87.1 kg [IQR 77.0; 100.2] 82.3 [72.1; 94.3] resulting...
Perinatal smoke/nicotine exposure alters lung development and causes asthma in exposed offspring, transmitted transgenerationally. The mechanism underlying the transgenerational inheritance of perinatal smoke/nicotine-induced remains unknown, but germline epigenetic modulations may play a role. Using well-established rat model nicotine-induced asthma, we determined DNA methylation pattern spermatozoa F1 rats perinatally to nicotine F0 gestation. To identify differentially methylated regions...
Although high-protein diets appear to be the most efficient way lose weight, concerns may arise about their innocuity on renal function. The objective of this study is assess impact a weight loss program A multicentric cohort-based was performed using RNPC© French national program. Patients with at least two creatinine measurements beginning and end phase between 1 January 2016 July 2021 were included. Renal function assessed by Modification Diet in Disease (MDRD) equation-based estimated...
Behavioural weight loss programmes are generally accepted as being beneficial in reducing cardiometabolic risk and improving patient-reported outcomes. However, prospective data from large real-world cohorts scarce concerning the mid-term long-term impact of such interventions. The objective this cohort study (n>10 000 participants) is to demonstrate effectiveness standardised Nutritional Psycho-Behavioural Rehabilitation programme (RNPC Programme) percentage subjects requiring insulin...
Obstructive sleep apnea (OSA) is one of the most frequent chronic diseases, and comorbid obesity occurs in more than 60% cases. Variations body weight influence both OSA severity OSA-related symptoms. We prospectively assessed impact a weight-loss program using Berlin score to reflect risk, we also used Epworth Sleepiness Scale (ESS) assess daytime sleepiness. DietSleep was prospective multicentric cohort study investigating risk sleepiness before after intervention. One hundred twenty-seven...
One of the major insulin resistance instigators is excessive adiposity and visceral fat depots. Individually, exercise training polyphenol intake are known to exert health benefits as improving sensitivity. However, their combined curative effects on established obesity need further investigation particularly white adipose tissue alterations. Therefore, we compared different depot alterations a combination grape supplementation in obese insulin-resistant rats fed high-fat diet alone or...
<title>Abstract</title> Systematic screening for liver fibrosis using FIB-4 score is recommended in primary care patients with chronic disease risk factors. This study assesses the prevalence and characteristics of at advanced a weight loss program. multicenter retrospective cohort includes obese overweight subjects participating program across 100 French centers. 27 643 participants baseline available were included, predominantly women (78.3%), median age 54 years, 70% obese. Baseline...