Probes based on anthra[1,2-d]imidazole-6,11-dione were designed and synthesized for selective ion sensing. Each probe acted as strong colorimetric sensors fluoride cyanide ions exhibited intramolecular charge transfer (ICT) band, which showed significant red-shifts after addition of either the F(-) or CN(-) ion. One probes (2) sensing both ions. In organic medium, 2 color change with cyanide, whereas in aqueous medium it a ratiometric response selectively

We present the selective sensing of multiple transition metal ions in water using a synthetic single probe. The probe is made up pyrene and pyridine as signaling interacting moiety, respectively. sensor showed different responses toward just by varying medium detection. In organic solvent (acetonitrile), detection Hg2+ ion. water, fluorescence quenching was observed with three ions, Cu2+, Hg2+, Ni2+. Further, surface charge on micellar aggregates, could detect discriminate above-mentioned...

The filovirus Ebola (EBOV) causes the most severe hemorrhagic fever known. EBOV RNA-dependent polymerase complex includes a filovirus-specific VP30, which is critical for transcriptional but not replication activity of polymerase; to support transcription, VP30 must be in dephosphorylated form. Here we show that phosphorylated only at N-terminal serine clusters identified previously also threonine residues positions 143 and 146. We host cell protein phosphatase 1 (PP1) controls...

New rhodamine based molecules have been designed as dual probes for the ppb-level selective detection of Hg2+ and F− ions in aqueous medium at physiological pH 7.4. The such a way to utilize both properties metal ion induced ‘turn-on’ mechanism spirolactam ring opening moiety reaction cleavage O-silyl bond presence fluoride ion. synthesized conveniently by coupling hydrazone with protected mono- di-hydroxybenzaldehydes. Both showed due upon treatment added However, ring. two mechanisms...

Abstract: Despite the remarkable success of combination antiretroviral therapy at curtailing HIV progression, emergence drug-resistant viruses, chronic low-grade inflammation, and adverse effects treatments, including metabolic disorders collectively present impetus for development newer safer drugs. Curcumin, a phytochemical compound, was previously reported to have some in vitro anti-HIV anti-inflammatory activities, but poor bioavailability has limited its clinical utility. To circumvent...

Abstract A new bis‐indolyl‐based colorimetric probe has been synthesized. This allows a Michael‐type adduct formation for the detection of cyanide ions. The shows remarkable color change from red to colorless upon addition ions in pure water. ion reacts with and removes conjugation bis‐indolyl moiety that 4‐substituted aromatic ring. renders colorless. mechanism reaction was established by using 1 H 13 C NMR spectroscopy, mass spectrometry, kinetic studies.

Cation sensing properties of the three positional isomers rhodamine based sensors (1–3) are studied in water. The differ only position pyridine's nitrogen. chemosensor 1, with pyridine nitrogen at ortho-position, showed a selective colorimetric detection Cu(II) ions water, physiological pH 7.4 and also medium containing BSA (bovine serum albumin) blood serum. Notably compound 2 3, end located meta- para-positions did not show any color change ions, although both compounds turn-on...

Abstract A new molecular probe based on an oxidized bis‐indolyl skeleton has been developed for rapid and sensitive visual detection of cyanide ions in water also the endogenously bound cyanide. The allows “naked‐eye” with a color change from red to yellow (Δ λ max =80 nm) immediate addition probe. It shows high selectivity towards ion without any interference other anions. by is ratiometric, thus making quantitative. Michael‐type reaction takes place during this chemodosimetric process. In...

Objectives Human immunodeficiency virus (HIV)-associated opportunistic fungal infections (OFI) are a major cause of mortality and morbidity in HIV-seropositive patients. This prospective study aimed to isolate various pathogens from HIV seropositive patients identify characterize these at the species level India. Material Methods Based on clinical signs symptoms, specimens (n=323) were collected (n=200) adult age group either sex underwent direct microscopy culture. Fungal isolates...

HIV-1 transcription is activated by the viral Tat protein that recruits host positive elongation factor-b (P-TEFb) containing CDK9/cyclin T1 to promoter. P-TEFb in cells exists as a lower molecular weight dimer and high complex of 7SK RNA, T1, HEXIM1 several additional proteins. Our previous studies implicated CDK2 regulation. We also found inhibition iron chelators leads CDK9 activity, suggesting functional link between CDK9. Here, we investigate whether phosphorylates regulates its...

Despite efficient antiretroviral therapy, eradication of HIV-1 infection is challenging and requires novel biological insights therapeutic strategies. Among other physiological environmental factors, intracellular iron greatly affects replication. Higher stores were shown to be associated with faster progression inversely correlate the survival infected patients. Iron required for several steps in life cycle, including reverse transcription, gene expression capsid assembly. Here, authors...

Highly active antiretroviral (ARV) therapy (HAART) for chronic suppression of HIV replication has revolutionized the treatment HIV/AIDS. HAART is no panacea; treatments must be maintained life. Although great progress been made in ARV therapy, continues to replicate anatomical and intracellular sites where drugs have restricted access. Nanotechnology considered a platform circumvent some challenges HIV/AIDS treatment. Dispersion polymerization was used fabricate two types (PMM ECA) polymeric...

A new colorimetric probe has been developed for the detection and estimation of Pd(II) at sub-nanomolar concentrations. The consisted rhodamine (signaling unit), which was linked with a bis-picolyl moiety (binding site) through phenyl ring. induced opening spirolactam ring generation prominent pink color. excellent selectivity towards over Pd(0) or Rh(II) ensured its potential utility residual palladium contamination in pharmaceutical drugs Pd-catalyzed reactions. showed "turn-on" (bright...

Cys34 albumin redox modifications (reversible "cysteinylation" and irreversible "di/trioxidation"), besides being just oxidative stress biomarkers, may have primary pathogenetic roles to initiate and/or aggravate cell, tissue, vascular damage in diabetes. In an exploratory "proof-of-concept" pilot study, we examined longitudinal changes oxidation during diabetes therapy.

HIV-1 transcription is activated by the Tat protein which recruits cyclin-dependent kinase CDK9/cyclin T1 to TAR RNA. binds phosphatase-1 (PP1) through Q(35) VCF(38) sequence and translocates PP1 nucleus. dephosphorylates CDK9 activates transcription. We have synthesized a low MW compound 1H4, that targets prevents interaction with inhibits gene Here, we report our further work 1H4-derived compounds analysis of their mechanism action.Using 1H4-PP1 complex as model, iteratively designed...

This communication describes a disassembly based approach for the detection of biologically relevant di- and tri-phosphates in water using locked fluorescent salicylaldehyde probes.

The low incidence of HIV-1 infection in patients with sickle cell disease (SCD) and inhibition replication vitro under the conditions intracellular iron or heme treatment suggests a potential restriction SCD. We investigated ex vivo SCD peripheral blood mononuclear cells (PBMCs) found that was inhibited at level reverse transcription (RT) transcription. observed increased expression iron-regulated genes, previously shown to inhibit HIV-1, including ferroportin, IKBα, HO-1, p21, SAM domain HD...

HIV-1 escapes antiretroviral drugs by integrating into the host DNA and forming a latent transcriptionally silent provirus. This provirus presents major hurdle in eradication cure. Transcriptional activation, which is prerequisite for reactivation of proviruses, impaired latently infected T cells due to lack transcription factors, primarily NF-κB P-TEFb (CDK9/cyclin T1). We others previously showed that protein phosphatase-1 (PP1) regulates modulating CDK9 phosphorylation. Recently we have...

Abstract A novel colorimetric probe 1 based on the picolyl moiety has been designed and synthesized. Probe is composed of a pyrene bispicolyl amine (BPA) unit, in which BPA acts as binding unit phenomenon sensed from changes signaling subunit. The detects Cu 2+ specifically water both Hg efficiently neutral Brij‐58 micellar media. shows color change visible to naked eye upon addition metal ions. Notably, medium, can detect ions even at parts‐per‐billion levels. Furthermore, ratiometric...

Sickle cell disease (SCD) is characterized by hemolysis, vaso-occlusion, and ischemia. HIV-1 infection was previously shown to be suppressed in SCD PBMCs. Here, we report that suppression attributed the increased expression of iron, hypoxia, interferon-induced innate antiviral factors. Inhibition upregulated genes,