Oral squamous cell carcinoma (OSCC), with high potential for metastasis, is the most common malignant tumor of head and neck. Cancer‑associated fibroblasts (CAFs) are main stromal cells in microenvironment aggravate progression. However, whether CAFs associated progression OSCC remains unknown underlying mechanism unclear. In present study, role mediating migration invasion was investigated, participation exosomal miR‑382‑5p this process elucidated. according to α‑SMA staining...

Gemcitabine (GEM)-based chemotherapy is the first-line treatment for locally advanced pancreatic cancer. GEM resistance, however, remains a significant clinical challenge. Here, we investigated whether exosomes derived from GEM-resistant cancer stem cells (CSCs) mediate cell-cell communication between that are sensitive or resistant to and, by doing so, regulate drug resistance.GEM-sensitive BxPC-3-derived BxS and PANC-1 were cultured with extracted CSCs isolated BxR (BxR-CSC). The effect of...

It is important to understand the unmet needs of family caregivers advanced cancer patients for developing and refining services address identified gaps in care. To explore their Chinese mainland possible factors associated with needs, a self-developed questionnaire was used survey sample 649 participants 15 hospitals Shanghai. The data were analysed using descriptive statistics, factor analysis, t-test, one-way ANOVA, Fishers least significant difference t-test. All statistical analyses...

BIX-01294, an euchromatic histone-lysine N-methyltransferase 2 (EHMT2) inhibitor, has been reported to induce apoptosis in human neuroblastoma cells and inhibit the proliferation of bladder cancer cells. However, definite mechanism mediated by BIX-01294 remains unclear. In present study, we found that induced caspase-dependent Moreover, our data show stimulates endoplasmic reticulum stress (ER stress) up-regulated expression PMAIP1 through DDIT3 up-regulation. Furthermore, down-regulation...

Abstract Gentamicin may cause acute kidney injury. The pathogenesis of gentamicin nephrotoxicity is unclear. Autophagy a highly conserved physiological process involved in removing damaged or aged biological macromolecules and organelles from the cytoplasm. role autophagy miniature pigs are more similar to humans than those rodents thus they suitable as human disease models. Here we established first model pigs, investigated gentamicin-induced injury determined prevention potential rapamycin...

Chemical exchange saturation transfer (CEST) signals at 3 ppm acquired with high irradiation powers, termed GluCEST, have been suggested as an indicator of glutamate in neural systems. However, amines proteins may also contribute to the GluCEST signal, but they ignored previous studies. This study aims investigate molecular origins and specificity signal rat brain.Dialysis was used selectively remove small molecules, such other metabolites, from tissue homogenates prepared brain. approach...

Neddylation is essential for cardiomyocyte survival in the presence of oxidative stress, and it participates autophagy regulation. However, whether MLN4924—an inhibitor neddylation—exerts cardioprotective effects against myocardial ischemia/reperfusion (MI/R) remains unknown. In present study, MLN4924 exerted strong effects, demonstrated by significantly elevated cell viability, a decreased LDH leakage rate, improved morphology following H2O2-induced injury vitro. also markedly serum...

Purpose Chemical exchange saturation transfer signals from amines are sensitive to pH, and detection of these can serve as an alternative pH imaging method amide proton (APT). However, conflicting results regarding amine CEST at 2 ppm in ischemic stroke have been reported. Here, we correlated with APT animal models evaluate its specificity investigated the reason for different through simulations sample studies. Methods A three‐point quantification was used quantify APT. polynomial fit a...

The purpose is to evaluate the relative contribution from confounding factors (T1 weighting and magnetization transfer) CEST ratio (CESTR)-quantified amide proton transfer (APT) nuclear Overhauser enhancement (NOE) (-3.5) in tumors as well whether CESTR can reflect distribution of solute concentration (fs ).We first provided a signal model that shows separate dependence on these clean CEST/NOE effects quantified by an apparent exchange-dependent relaxation (AREX) method. We then measured...

Abstract The energy sector is going through changes aiming at the creation of new sources and improvement existing ones to meet increasing demand. New have appeared, giving cleaner renewable choices, but same time, many difficulties emerged, like irregular supply low density. This leads importance multi-energy technologies maintain efficiency stability. research used Thermoflow software predict power plant performance, showing a good match with pre-production data confirming accuracy....

Accurate femoral tunnel positioning is crucial for successful anterior cruciate ligament reconstruction (ACLR), yet traditional arthroscopic techniques face significant challenges in spatial orientation and precise anatomical localization. This study presents a novel marker-less computer-assisted navigation system that integrates three-dimensional modeling with real-time guidance. The employs advanced image processing accurate condyle segmentation implements the Bernard & Hertel...

The accelerating conversion of agricultural land to non-agricultural uses poses critical threats food security and sustainable management, particularly in ecologically fragile karst mountainous regions. This study investigated the spatiotemporal patterns driving mechanisms cropland (CNAC) Qian-Gui region (Guangxi Guizhou, China) from 2000 2020, employing use datasets socioeconomic indicators through geographically weighted regression (GWR) modeling. results showed that (1) CNAC rate...

Abstract Background To establish a prognostic risk profile for ovarian cancer (OC) patients based on cancer-associated fibroblasts (CAFs) and gain comprehensive understanding of their role in OC progression, prognosis, therapeutic efficacy. Methods Data single-cell RNA sequencing (scRNA-seq) total RNA-seq were collected from the GEO TCGA databases. Seurat R program was used to analyze scRNA-seq data identify CAFs clusters corresponding markers. Differential expression analysis performed...