A5101590655- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Plant Molecular Biology Research
- Pancreatic and Hepatic Oncology Research
- Cancer, Lipids, and Metabolism
- Lipid metabolism and disorders
- Cancer-related molecular mechanisms research
- Growth Hormone and Insulin-like Growth Factors
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA Research and Splicing
- Cancer Research and Treatments
- Analytical Chemistry and Chromatography
- Extracellular vesicles in disease
- Medicinal Plant Pharmacodynamics Research
- Hedgehog Signaling Pathway Studies
- Cancer-related Molecular Pathways
- Hepatocellular Carcinoma Treatment and Prognosis
- Peptidase Inhibition and Analysis
- Traditional Chinese Medicine Analysis
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Plant Virus Research Studies
Zhongnan Hospital of Wuhan University
2019-2025
Wuhan University
2019-2025
First Affiliated Hospital of GuangXi Medical University
2025
Guangxi Medical University
2025
Huanggang Central Hospital
2015-2022
Guangdong Provincial People's Hospital
2020-2021
Yangtze University
2021
The First Hospital of Changsha
2020
Air Force General Hospital PLA
2016
Monash University
2001-2015
Methylation on the base or ribose is prevalent in eukaryotic ribosomal RNAs (rRNAs) and thought to be crucial for ribosome biogenesis function. Artificially introduced 2'-O-methyl groups small interfering (siRNAs) can stabilize siRNAs serum without affecting their activities RNA interference mammalian cells. Here, we show that plant microRNAs (miRNAs) have a naturally occurring methyl group of last nucleotide. Whereas methylation rRNAs depends guide RNAs, methyltransferase protein HEN1...
Gemcitabine (GEM)-based chemotherapy is the first-line treatment for locally advanced pancreatic cancer. GEM resistance, however, remains a significant clinical challenge. Here, we investigated whether exosomes derived from GEM-resistant cancer stem cells (CSCs) mediate cell-cell communication between that are sensitive or resistant to and, by doing so, regulate drug resistance.GEM-sensitive BxPC-3-derived BxS and PANC-1 were cultured with extracted CSCs isolated BxR (BxR-CSC). The effect of...
Pancreatic cancer (PC) remains a primary cause of cancer-related deaths worldwide. Existing literature has highlighted the oncogenic role microRNA-27a (miR-27a) in multiple cancers. Hence, current study aimed to clarify potential therapeutic PC cell-derived exosomal miR-27a human microvascular endothelial cell (HMVEC) angiogenesis PC. Initially, differentially expressed genes (DEGs) and miRs related were identified by microarray analysis. Microarray analysis provided data predicting...
Indocyanine green (ICG) fluorescence imaging technology is increasingly widely used in laparoscopic hepatectomy. However, whether it can provide long-term survival benefits to patients with liver malignancies remains unclear. This study investigated the clinical effect of hepatectomy for hepatocellular carcinoma (HCC) using ICG technology. We retrospectively analyzed HCC who underwent at Zhongnan Hospital Wuhan University from January 2016 December 2020. Propensity score matching (PSM) was...
HEN1‐dependent methylation of the 3′‐terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC‐Pro, p21 and p19) inhibit miRNA methylation. These have distinct effects on different miRNAs. We also show ∗ methylated vivo suppressor‐sensitive manner, suggesting proteins interfere with miRNA/miRNA duplexes. p19 bind both unmethylated duplexes vivo. findings suggest as substrates for HEN1 methyltransferase raise...
The progression of cancer depends on the establishment a tumour blood supply, and therefore angiogenesis has been identified as major target for new anticancer agents. Recent reports have suggested that osteoprotegerin (OPG) is involved in control endothelial cell survival through inhibition activity necrosis factor- (TNF) related apoptosis-inducing ligand (TRAIL). role OPG human development currently unknown. In present study we demonstrate ability to support survival, well formation...
Abstract Understanding the genetic variation underlying transcript splicing is essential for fully dissecting molecular mechanisms of common diseases. The available evidence from quantitative trait locus (sQTL) studies using pancreatic ductal adenocarcinoma (PDAC) tissues have been limited to small sample sizes. Here we present a genome-wide sQTL analysis identify SNP that control mRNA in 176 PDAC samples TCGA. From this analysis, 16,175 sQTLs were found be significantly enriched RNA-binding...
ABSTRACT Background Early‐stage pancreatic ductal adenocarcinoma (PDAC) is frequently misdiagnosed, contributing to its high mortality rate. Exosomal microRNAs (miRNAs) have emerged as potential biomarkers for the early detection of PDAC. Aims This study aimed evaluate feasibility using exosomal miRNAs from PDAC tissues and serum prognosis. Materials & Methods Exosomes were isolated healthy individuals patients via tissue samples, then identified by analyzing their particle size protein...
Introduction Talaromyces marneffei ( T. ) infections in children can occur secondary to inborn errors of immunity (IEIs). We aimed investigate the clinical and genetic features infection Chinese pediatric patients. Materials methods retrospectively reviewed 18 patients with IEIs who were diagnosed at five public hospitals China from January 2015 2023. Results The common among fever, cough, hepatomegaly. most severe complications included septic shock, hemophagocytic lymphohistiocytosis...
<i>Background/Aims:</i> The polycomb protein Bmi-1 plays oncogenic roles in various cancers. Here we aimed to investigate the contribution of on malignant behaviors pancreatic cancer such as chemoresistance, invasion and tumorigenesis. <i>Methods Results:</i> MTT cell proliferation assay showed that shRNA mediated knockdown enhanced chemosensitivity cells gemcitabine. transwell inhibited vitro. Notably, reduced abilities chemoresistance were associated with transition...
Rationale: Understanding the roles of small nucleolar RNAs (snoRNAs) in hepatocarcinogenesis will provide new avenues to identify diagnostic and therapeutic targets for hepatocellular carcinoma (HCC). Our previous research confirmed tumor-suppressive effect Up-frameshift 1 (Upf1) HCC. Herein, we examined expression profiles snoRNAs regulated by Upf1 hepatoma cells. Methods: We cells using RNA-sequencing analysis then investigated significance SNORD52 HCC tissue different cell lines. The...
Pancreatic ductal adenocarcinoma (PDAC) has high mortality and poor prognosis. Pyroptosis can influence the prognosis of patients by regulating proliferation, invasion, metastasis cancer cells. However, role pyroptosis-related genes (PRGs) in PDAC remains unclear.In this study, based on Cancer Genome Atlas (TCGA) cohort samples, univariate Cox analysis LASSO regression were used to screen prognostic PRGs establish gene signature. To further evaluate functional significance CASP4 NLRP1 PDAC,...
P-glycoprotein (P-gp) is a major factor in multidrug resistance (MDR) which serious obstacle chemotherapy. P-gp has also been implicated causing apoptosis of tumor cells, was shown to be another important mechanism MDR recently. To study the influence cell apoptosis, K562/A cells (P-gp+) and K562/S (P-gp−) were subjected doxorubicin (Dox), serum withdrawal, or independent co-incubation with multiple inhibitors, including valspodar (PSC833), verapamil (Ver) H108 induce apoptosis. Apoptosis...
IGFBP-6 inhibits angiogenesis as well proliferation and survival of rhabdomyosarcoma cells. However, it promotes migration these cells in an IGF-independent manner. The IGF system is implicated ovarian cancer, so we studied the effects cancer cells.The wild type (wt) a non-IGF-binding mutant (m) on HEY SKOV3 cells, which, respectively, represent aggressive transitional cancers, were studied. ERK JNK phosphorylation measured by Western blotting.IGF-II, wt-, mIGFBP-6 each promoted cell 77-98%...
PurposeThe clinical values of C-reactive protein (CRP) and serum amyloid A (SAA) to distinguish non-severe from severe influenza in children are rarely reported.MethodsBaseline characteristics laboratory results were collected analyzed. Receiver operating characteristic (ROC) curve analysis was used for combined detection indicators with influenza, scatter-dot plots compare the differences between influenza.ResultsChildren B had more bronchitis pneumonia (P < 0.05) other serious symptoms =...
Abstract Background Sphingosine-1-phosphate receptor ( S1PR1 ) is involved in vascular development, a key process tumorigenesis. This study aimed to evaluate its roles tumor development and prognosis. Methods expression levels were analyzed using TIMER Oncomine database, the prognostic significance of was assessed PrognoScan Kaplan-Meier plotter databases. The relationship between tumor-infiltrated immune cells TIMER. Results remarkably lower breast lung cancer tissues than corresponding...