A5041552982- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Platelet Disorders and Treatments
- Circadian rhythm and melatonin
- Genetics, Aging, and Longevity in Model Organisms
- Immune Cell Function and Interaction
- Food Allergy and Anaphylaxis Research
- Blood disorders and treatments
- Blood groups and transfusion
- Genetic and Kidney Cyst Diseases
- Allergic Rhinitis and Sensitization
- Immunotherapy and Immune Responses
- IgG4-Related and Inflammatory Diseases
- Signaling Pathways in Disease
- Insect Utilization and Effects
- Erythrocyte Function and Pathophysiology
- Animal and Plant Science Education
- HER2/EGFR in Cancer Research
- Mitochondrial Function and Pathology
- Complement system in diseases
- Kawasaki Disease and Coronary Complications
- Diabetes and associated disorders
- Food Safety and Hygiene
- Insect and Arachnid Ecology and Behavior
Wageningen University & Research
2023-2024
Vrije Universiteit Amsterdam
2022-2023
Sanquin
2017-2020
University of Amsterdam
2017-2020
Amsterdam UMC Location University of Amsterdam
2017-2019
Amsterdam University Medical Centers
2019
Walter and Eliza Hall Institute of Medical Research
2017
The function of the low-affinity IgG-receptor FcγRIIIb (CD16b), which is uniquely and abundantly expressed on human granulocytes, not clear. Unlike other Fcγ receptors (FcγR), it a glycophosphatidyl inositol (GPI) -anchored molecule does have intracellular signaling motifs. Nevertheless, can cooperate with FcγR to promote phagocytosis antibody-opsonized microbes by neutrophils. Here we investigated role during antibody-dependent cellular cytotoxicity (ADCC) neutrophils towards solid cancer...
Abstract Abs of the IgG isotype are glycosylated in their Fc domain at a conserved asparagine position 297. Removal core fucose this glycan greatly increases affinity for FcγRIII, resulting enhanced FcγRIII-mediated effector functions. Normal plasma contains ∼94% fucosylated Abs, but alloantibodies against, example, Rhesus D (RhD) and platelet Ags frequently have reduced fucosylation that enhances pathogenicity. The increased functions been put to use various afucosylated therapeutic...
Ab-dependent cellular cytotoxicity (ADCC) is one of the most important effector mechanisms tumor-targeting Abs in current immunotherapies. In ADCC and other activation myeloid cells, close cell-cell contact (between target cell) formation immunological synapses are required. However, we still lack basic knowledge on principal factors influencing potential by therapeutic Abs. this study investigated combined roles five affecting human NK cell-mediated ADCC, namely: 1) Ag density, 2) cell...
Recently it was discovered that tissue-resident macrophages derive from embryonic precursors, not only peripheral blood monocytes, and maintain themselves by self-renewal. Most in-vitro studies on macrophage biology make use of cultured human monocyte-derived macrophages. Phagocytosis IgG-opsonized particles takes place via interaction with IgG receptors, the Fc-gamma receptors (FcγRs). We investigated FcγR expression both in-vivo ex-vivo different tissues. Upon isolation primary bone...
Abstract Hemolytic anemia resulting from IV Immunoglobulin (IVIG) treatment can be a serious complication, especially for those with underlying conditions high level of inflammation and after administration IVIG dosages, such as Kawasaki disease (KD), multisystem vasculitis affecting young children. This hemolysis is caused by antibodies against blood groups A B, but the precise mechanism not known. We performed single center, partly retrospective, prospective study cohort 581 patients who...
Intravenous immunoglobulins (IVIg) are used in the treatment of different autoimmune and inflammatory diseases, amongst which immune thrombocytopenia hemolytic anemia. One modes action IVIg is preventing phagocytosis autoantibody-opsonized blood cells by saturation Fc-gamma receptors macrophages spleen liver. IgG contains a fixed biantennary glycan at asparagine residue position 297 Fc tail. This consists core structure N-acetyl glucosamine (GlcNAc) mannose groups, variably extended with...
Here, we present a protocol to use microfluidics in combination with fluorescence microscopy expose the C. elegans tail chemosensory stimuli. We describe steps for preparation of microfluidic chips and sample through sedation elegans. detail flow calibration imaging determine their molecular and/or cellular response This can also be applied amphid neurons by inserting worm chip head-first. For complete details on execution this protocol, please refer Bruggeman et al. (2022).1.
Insects are increasingly used as an alternative protein source for feed and food production. One of the main biological hazards associated with edible insects is bio-accumulation foodborne pathogenic microorganisms. In this study we explored interaction larvae black soldier fly (BSFL, Hermetia illucens (L.), Diptera: Stratiomyidae) pathogen Bacillus cereus. order to do so, reared BSFL on a substrate which was inoculated either B. cereus vegetative cells or spores. After seven days rearing,...
Summary Cilia are membrane-enveloped organelles that protrude from the surface of most eurokaryotic cells and play crucial roles in sensing external environment. For maintenance function cilia dependent on intraflagellar transport (IFT). Here we use a combination microfluidics fluorescence microscopy to study response phasmid chemosensory neurons, live Caenorhabditis elegans , chemical stimuli. We found stimulation resulted unexpected changes IFT ciliary structure. Notably, with hyperosmotic...