A5080536451- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Effects and risks of endocrine disrupting chemicals
- Toxic Organic Pollutants Impact
- Cancer, Lipids, and Metabolism
- RNA Interference and Gene Delivery
- Estrogen and related hormone effects
- Prostate Cancer Treatment and Research
- RNA Research and Splicing
- Carcinogens and Genotoxicity Assessment
- Nuclear Structure and Function
- Advanced biosensing and bioanalysis techniques
- Mass Spectrometry Techniques and Applications
- HER2/EGFR in Cancer Research
- Air Quality and Health Impacts
- Cell Adhesion Molecules Research
- Cell death mechanisms and regulation
- DNA Repair Mechanisms
- Genomic variations and chromosomal abnormalities
- Per- and polyfluoroalkyl substances research
- Nitric Oxide and Endothelin Effects
- BRCA gene mutations in cancer
- Ocular Oncology and Treatments
- Hippo pathway signaling and YAP/TAZ
University of Cincinnati
2010-2024
University of Cincinnati Medical Center
2005-2021
Center for Environmental Health
2017
Center for Human Genetics
2013
Oncology Hematology Care
2012
The kinase activity of cyclin-dependent 2 (CDK2)-cyclin E is required for centrosomes to initiate duplication. We have recently found that nucleophosmin (NPM/B23), a phosphoprotein primarily in nucleolus, associates with unduplicated and direct substrate CDK2-cyclin centrosome Upon phosphorylation by E, NPM/B23 dissociates from centrosomes, which prerequisite step Here, we identified threonine 199 (Thr<sup>199</sup>) the major target site <i>in vitro</i>, same phosphorylated vivo</i>....
Human exposure to bisphenol A (BPA) is ubiquitous. Animal studies found that BPA contributes development of prostate cancer, but human data are scarce. Our study examined the association between urinary levels and Prostate cancer assessed effects on induction centrosome abnormalities as an underlying mechanism promoting carcinogenesis. The study, involving 60 urology patients, higher (creatinine-adjusted) in patients (5.74 µg/g [95% CI; 2.63, 12.51]) than non-Prostate (1.43 0.70, 2.88]) (p =...
Abstract The liver exhibits an exquisitely controlled cell cycle, wherein hepatocytes are maintained in quiescence until stimulated to proliferate. retinoblastoma tumor suppressor, pRB, plays a central role proliferative control by inhibiting inappropriate cycle entry. In many cases, cancer arises due aberrant cycles of proliferation, and correspondingly, pRB is functionally inactivated the majority hepatocellular carcinomas. Therefore, determine how loss may provide conditions permissive...
Humans are increasingly exposed to structural analogues of bisphenol A (BPA), as BPA is being replaced by these compounds in BPA-free consumer products. We have previously shown that chronic and developmental exposure associated with increased prostate cancer (PCa) risk human animal models. Here, we examine whether PCa cells (LNCaP, C4-2) low-dose its (BPS, BPF, BPAF, TBBPA, DMBPA TMBPA) affects centrosome amplification (CA), a hallmark initiation progression. found BPA, BPS, descending...
Ovarian cancer is a highly metastatic and lethal disease, making it imperative to find treatments that target late-stage malignant tumors. The packaging RNA (pRNA) of bacteriophage phi29 DNA-packaging motor has been reported function as versatile vehicle carry small interference (siRNA) for silencing survivin. In this article, we explore the potential pRNA siRNA specifically targeted metallothionein-IIa (MT-IIA) messenger (mRNA), compare survivin targeting pRNA. These two anti-apoptotic cell...
Nucleophosmin (NPM)/B23 is a multifunctional protein, involving in wide variety of basic cellular processes, including ribosome assembly, DNA duplication, nucleocytoplasmic trafficking, and centrosome duplication. It has previously been shown that NPM/B23 localizes to centrosomes, dissociate from centrosomes upon phosphorylation by Cdk2/cyclin E. However, detail characterization centrosomal association hampered the lack appropriate antibodies efficiently detects centrosomally localized...
Centrosome duplication in mammalian cells is a highly regulated process, occurs coordination of other cell cycle events. However, molecular exploration this important cellular process had been difficult due to unavailability simple assay system. Here, using centrosomes loosely associated with nuclei isolated from cultured cells, we developed cell-free centriole (duplication unit the centrosome) system: unduplicated bound are able undergo presence G1/S extracts. We show that ability extracts...
Nucleophosmin (NPM) is a multifunctional phosphoprotein, being involved in ribosome assembly, pre-ribosomal RNA processing, DNA duplication, nucleocytoplasmic protein trafficking, and centrosome duplication. NPM phosphorylated by several kinases, including nuclear kinase II, casein 2, Polo-like 1 cyclin-dependent kinases (CDK1 2), these phosphorylations modulate the activity function of NPM. We have previously identified Thr(199) as major phosphorylation site mediated CDK2/cyclin E (and A),...
Alternative splicing of estrogen receptor β (ERβ) yields five isoforms, but their functions remain elusive. ERβ isoform5 (ERβ5) has been positively correlated with better prognosis and longer survival patients breast cancer (BCa) in various clinical studies. In this study, we investigated the inhibitory role ERβ5 BCa cells. Although does not reduce proliferation cell lines MCF-7 MDA-MB-231, its ectopic expression significantly decreases by sensitizing them to doxorubicin- or...
BRCA1, a product of familial breast and ovarian cancer susceptibility gene, localizes to centrosomes physically interacts with γ-tubulin, key centrosomal protein for microtubule nucleation anchoring at centrosomes. Here, we performed rigorous analysis centrosome localization found that BRCA1 is specifically associated mother centrioles in unduplicated centrosomes, daughter acquire prior initiation duplication, thus duplicated are both bound by BRCA1. We further suppresses aster formation. In...
The Ski oncoprotein has been found to bind non-specifically DNA in association with unindentified nuclear factors. In addition, shown activate transcription of muscle-specific and viral promoters/enhancers. present study was undertaken identify Ski's binding transcriptional activation partners by identifying specific sites. We used extracts from a v-Ski-transduced mouse L-cell line selected Ski-bound sequences pool degenerate oligonucleotides anti-Ski monoclonal antibodies. Two were...
The increased mortality in prostate cancer is usually the result of metastatic progression disease from organ-confined location. Among major events this cascade are enhanced cell migration and loss adhesion. Moreover, elevated levels nitric oxide (NO) inducible synthase (iNOS) found within tumor microenvironment hallmarks cancer. To understand role nitrosative stress progression, we investigated effects NO iNOS on Our results indicate that ectopic expression cells extent migration, which...