A5050262032- PARP inhibition in cancer therapy
- Liver Disease Diagnosis and Treatment
- Toxin Mechanisms and Immunotoxins
- Osteoarthritis Treatment and Mechanisms
- Inflammatory Bowel Disease
- Calcium signaling and nucleotide metabolism
- Immune Response and Inflammation
- Ion channel regulation and function
- Helicobacter pylori-related gastroenterology studies
- Alcohol Consumption and Health Effects
- Microscopic Colitis
- Immune Cell Function and Interaction
- Liver Diseases and Immunity
- Digestive system and related health
- Inflammatory mediators and NSAID effects
- Metabolism and Genetic Disorders
- Diet and metabolism studies
- Pulmonary Hypertension Research and Treatments
- Glycosylation and Glycoproteins Research
- Photonic and Optical Devices
- Nitric Oxide and Endothelin Effects
- Rheumatoid Arthritis Research and Therapies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Amino Acid Enzymes and Metabolism
- Thermoregulation and physiological responses
Hamamatsu University School of Medicine
2017-2024
Seirei Hamamatsu General Hospital
2017-2022
Keio University
1990-2021
Juntendo University
2019
University of Yamanashi
2017
Toho University
2001-2016
Tokyo Metropolitan Geriatric Hospital
2013-2015
Juntendo University Hospital
2013-2015
National Institute of Technology, Miyakonojo College
2015
Tokyo Women's Medical University
2007
The interaction of mucosal lymphocytes and intestinal epithelial cells is thought to be important in regulating immune response the mucosa, but conclusive evidence limited. Here we demonstrate expression IL-7 mRNA human mucosa by combined reverse transcription PCR Southern blot hybridization. Immunohistochemistry situ hybridization confirm presence cells, especially goblet cells. Moreover, receptor demonstrated immunohistochemistry hybridization, as well flow cytometric analysis freshly...
Abstract ADP-ribosylation factors (ARFs) are approximately 20-kDa guanine nucleotide-binding proteins that serve as GTP-dependent allosteric activators of cholera toxin ADP-ribosyltransferase activity. Four species mammalian ARF, termed ARF 1-4, have been identified by cloning. Hybridization a bovine 2 cDNA under low stringency with poly(A)+ RNA resulted in multiple bands were subsequently assigned to the known genes using ARF-specific oligonucleotide probes. The relative signal intensities...
The role of neutrophil and its chlorinated oxidant were investigated in Helicobacter pylori-induced gastric mucosal injury vitro. Luminol-dependent chemiluminescence (ChL) was used to detect neutrophil-derived oxidants. ChL activity significantly elevated when neutrophils incubated H. pylori, indicating that pylori actually elicits oxidative burst neutrophils. To assess whether pylori-activated exert the cytotoxicity for cells, rabbit cell monolayered culture wells labeled with a...
Chromatin-bound ADP-ribosyltransferase from adult hen liver nuclei was purified to a homogeneous state through salt extraction, gel filtration, hydroxyapatite, phenyl-Sepharose, Cm-cellulose, and DNA-Sepharose.The has pH optimum at 9.0 does not require DNA for reaction.The enzyme molecular weight of 27,500 2 500.Agmatine sulfate, arginine methyl ester, histones, casein proved be effective acceptors the ADP-ribose molecule.Among H3 most active, followed by H2a, H4, H2b, in that order, lowest...
The effect of rebamipide, a novel antiulcer compound, on Helicobacter pylori activated neutrophil dependent in vitro gastric epithelial cell injury was investigated. Luminol chemiluminescence (ChL), which detects toxic oxidants from neutrophils exhibited 12-fold increase when the bacterial suspension H added to isolated human neutrophils. This change significantly attenuated by rebamipide at concentration less than 1 mM, showing that may inhibit oxidant production elicited To assess whether...
Participation of microcirculatory disturbances, especially the role 5-lipoxygenase products from neutrophils, was investigated in indomethacin (Indo)-induced ulcers rat small intestine. After Indo treatment (20 mg/kg) rats, erosions appeared at 6 h and longitudinal developed 12 later. At after treatment, severe disturbances were observed under an intravital fluorescence microscope. Significant delay clearance patchy pooling injected fluorescein isothiocyanate-bovine serum albumin with sludge...
Two arginine-specific ADP-ribosyltransferase cDNAs (designated AT1 and AT2) were cloned from chicken bone marrow cells. Each cDNA encodes a different peptide of 312 amino acid residues. Homology deduced sequences between AT2 was 78.3%. We found all six combined 222 residues derived purified heterophil (Mishima, K., Terashima, M., Obara, S., Yamada, Imai, Shimoyama, M. (1991) J. Biochem. (Tokyo) 110, 388-394) in the sequence AT1, with two mismatches. Arginine-specific activity detected...
Helicobacter pylori (HP) produces strong urease [EC 3.5.1.5], which is considered to play a role in the pathogenesis of gastritis and peptic ulcers. Inhibitions against this enzyme have been studied with hydroxamic acid (HXA) derivatives aliphatic or aromatic carboxylic acids, amino acids dipeptides. A number HXAs potently inhibited (I50 values were near order 10-6M), H-Ile-Gly-NHOH (I50=0.20×10-6M) was most potent inhibitor among derivatives. HP more potently, general, than Jack bean (JB)...
Cholera toxin exerts its effects on cells in large part through the ADP-ribosylation of guanine nucleotide-binding proteins. Toxin-catalyzed is enhanced by approximately 20-kDa proteins termed factors (ARFs), which are allosteric activators catalytic unit. Rabbit antiserum against a purified bovine brain ARF (sARF II) reacted immunoblots with two ARF-like I and tissue extracts from bovine, rat, frog, chicken. Levels were higher than non-neural tissues. In rat brain, second postnatal day,...
Silkworm larvae plasma (SLP) reagent is activated by peptidoglycan (PG), a fragment of both the gram-positive and gram-negative bacterial cell wall, as well beta-glucan (BG), component fungi. It possible to measure contamination bacteria quantitatively combining conventional limulus amebocyte lysate (LAL) PG measurement methods. Therefore, more highly accurate analysis dialysate can be made using SLP LAL methods detect endotoxin (ET) and/or contamination. We studied effects contaminated on...
Clones referred to as ARD 1 were isolated from human and rat cDNA libraries. genes encode a putative 64-kDa protein that contains an 18-kDa ADP-ribosylation factor (ARF) domain at the carboxyl terminus is much larger than other monomeric approximately 20-kDa guanine nucleotide-binding ARF proteins thus far identified. mRNAs of 3.7 4.1 kilobases detected in all tissues well mouse rabbit brain, fibroblasts, neuroblastoma cells but not HL-60 cells. Based on sequence identities, highly conserved...
The gastrointestinal involvement and immunological findings in 16 patients with Behçet's disease are described. Four of 15 biopsy specimens jejunal mucosa showed marked lymphangiectasia the lamina propria similar to appearance which was thought be a characteristic finding protein-losing enteropathy. None had hypoproteinaemia. Increases serum immunoglobulin IgA were proved six cases; IgM, five 15; IgG, one 15. Electron microscopic studies that there thousands precipitated lymph protein bodies...