A5059316602- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Analytical Chemistry and Sensors
- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Cellular Mechanics and Interactions
- Conducting polymers and applications
- Electrochemical sensors and biosensors
- Nanomaterials for catalytic reactions
- Advanced Biosensing Techniques and Applications
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Force Microscopy Techniques and Applications
- Molecular Junctions and Nanostructures
- Polymer Surface Interaction Studies
- Nanoparticles: synthesis and applications
- Analytical chemistry methods development
- Supramolecular Self-Assembly in Materials
- Bone Tissue Engineering Materials
- Diatoms and Algae Research
- MicroRNA in disease regulation
- Nanocluster Synthesis and Applications
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Adhesion, Friction, and Surface Interactions
Center for Advanced Biomaterials for Healthcare
2013-2024
Italian Institute of Technology
2015-2024
University of Chieti-Pescara
2023-2024
University of Naples Federico II
2012-2022
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi
2018-2022
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2017-2018
Federico II University Hospital
2018
Naples Anesthesia & Physician Associates
2018
Magna Graecia University
2010
Although lifetimes and quantum yields of widely used fluorophores are often largely characterized, a systematic approach providing rationale their photophysical behavior on quantitative basis is still challenging goal. Here we combine methods rooted in the time-dependent density functional theory fluorescence lifetime imaging microscopy to accurately determine analyze signatures (lifetime, yield, band peaks) several commonly rhodamine pyronin dyes. We show that radiative rhodamines can be...
We present novel microgels as a particle-based suspension array for direct and absolute microRNA (miRNA) detection. The feature flexible molecular architecture, antifouling properties, enhanced sensitivity with large dynamic range of Specifically, they possess core–shell architecture two different fluorescent dyes multiplex spectral analyses are endowed probe miRNA Encoding detection fluorescence signals distinguishable by nonoverlapping emission spectra. Tunable conjugation confinement on...
The cell recognition of bioactive ligands immobilized on polymeric surfaces is strongly dependent ligand presentation at the cell/material interface. While small peptide sequences such as Arg-Gly-Asp (RGD) are being widely used to obtain biomimetic interfaces, surface characteristics after immobilization well receptors deserve more detailed investigation. Here, we an RGD-based sequence poly(epsilon-caprolactone) (PCL), a largely widespread material in biomedical applications, polymer...
The requirement of a multifunctional scaffold for tissue engineering capable to offer at the same time tunable structural properties and bioactive interface is still unpaired. Here we present three-dimensional (3D) biodegradable polymeric (PCL) scaffolds with controlled morphology, macro-, micro-, nano-mechanical performances endowed moieties (RGD peptides) surface. Such result was obtained by combination rapid prototyping (e.g., 3D fiber deposition) surface treatment approach (aminolysis...
Biosensors are easy-to-use and cost-effective devices that emerging as an attractive tool, not only in settling diagnosis or disease monitoring, but also mass screening tests, a timely topic impacts on daily life of the whole society. Nanotechnologies lend themselves to development highly sensitive whose realization has become very interdisciplinary topic. Relying enhancement fluorescence signal detected at surface patterned gold nanoparticles, we report behavior analytical device detecting...
Polyethylenimine (PEI) is a cationic polymer able to interact with nucleic acids. Taking advantage of this property, PEI‐modified functional surfaces for the capture DNA were developed and characterized X‐ray photoelectron spectroscopy (XPS) during every step preparation. The XPS demonstrated presence PEI on surfaces, while density primary amino groups was quantified colorimetric test, resulting in 6 × 10 14 groups/cm 2 . Different amounts adsorbed interaction between monitored both via...
Fine-tuning the shape of nanostructured materials through easy and sustainable methods is a challenging task for green nanotechnology.
Advanced optical materials or interfaces are gaining attention for diagnostic applications. However, the achievement of large device interface as well facile surface functionalization largely impairs their wide use. The present work is aimed to address different innovative aspects related fabrication large-area 3D plasmonic arrays, direct and easy with capture elements, spectroscopic verifications through enhanced Raman fluorescence techniques. In detail, we have investigated effect a...
Synthetic receptors for biomacromolecules lack the supramolecular self-assembly behavior typical of biological systems. Here we propose a new method preparation protein imprinted polymers based on specific interaction peptide multi-functional block with target. This contains protein-binding domain, polymerizable moiety at C-terminus and an environment-sensitive fluorescent molecule N-terminus. The relies preliminary step consisting peptide/protein assembly, followed by copolymerization most...
In this study, a supramolecular structure with femtomolar biorecognition properties is proposed for use in analytical devices. It obtained by an innovative interface between synthetic hydrogel polymers and molecular beacon (mb) probes. Supramolecularly structured microgels are synthetized core-shell architecture specific dyes polymerized desired compartment. Mb probes opportunely conjugated at the microgel so that their recognition mechanism preserved spatial distribution optimized to avoid...
Abstract The question of whether material stiffness enhances cell adhesion and clustering is still open to debate. Results from the literature are seemingly contradictory, with some reports illustrating that increases surface others suggesting performance a system cells curbed by high values elasticity. To address role elasticity as regulator in neuronal clustering, we investigated topological characteristics networks neurons on polydimethylsiloxane (PDMS) surfaces - (E) varying 0.55–2.65...
A plethora of work has been dedicated to the analysis cell behavior on substrates with ordered topographical features. However, natural microenvironment is characterized by biomechanical cues organized over multiple scales. Here, randomly rough, self-affinefractal surfaces are generated out silicon,where roughness Ra and fractal dimension Df independently controlled. The proliferation rates, formation adhesion structures morphology 3T3 murine fibroblasts monitored six different substrates....
In this work, metal-ceramic nanocomposites were obtained through short (up to 2 h) thermal treatments at relatively moderate temperatures (750–800 °C) under a reducing atmosphere, using Fe-exchanged zeolite A as the precursor. The as-obtained materials characterized by X-ray powder diffraction analysis, N2 adsorption –196 °C, and highresolution transmission electron microscopy. results of these analyses showed that consisted dispersion metallic Fe nanoparticles within porous ceramic matrix,...