A5053465388- Cholesterol and Lipid Metabolism
- Metabolomics and Mass Spectrometry Studies
- Metabolism and Genetic Disorders
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Cystic Fibrosis Research Advances
- Drug Transport and Resistance Mechanisms
- Mass Spectrometry Techniques and Applications
- Peroxisome Proliferator-Activated Receptors
- Amino Acid Enzymes and Metabolism
- Analytical Chemistry and Chromatography
- Liver Disease Diagnosis and Treatment
- Lipoproteins and Cardiovascular Health
- Antioxidant Activity and Oxidative Stress
- Nitric Oxide and Endothelin Effects
- Cultural Heritage Materials Analysis
- Alzheimer's disease research and treatments
- SARS-CoV-2 detection and testing
- Steroid Chemistry and Biochemistry
- Diet and metabolism studies
- Building materials and conservation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Long-Term Effects of COVID-19
- Helicobacter pylori-related gastroenterology studies
- Fatty Acid Research and Health
University of Foggia
2016-2025
Ospedali Riuniti di Foggia
2020-2024
Ceinge Biotecnologie Avanzate (Italy)
1997-2022
University of Naples Federico II
1980-2019
Federico II University Hospital
2000-2010
University of Padua
2000
Universidade Estadual de Campinas (UNICAMP)
1999
University of Genoa
1999
University of Perugia
1997
Ospedale Pediatrico Giovanni XXIII
1997
The molecular basis of the wide clinical heterogeneity Coronavirus disease 2019 (COVID-19) is still unknown. Matrix metalloproteinases (MMPs) may have a role in lung damage and regeneration that occur severe patients. We studied serum MMP3 MMP9 as potential biomarkers COVID-19 severity, 108 hospitalized patients with different World Health Organization (WHO) severity stage 48 controls. At hospital admission, was increased significant trend along progression WHO stage, while levels were...
To evaluate the contribution of gluconeogenesis to epinephrine-stimulated glucose production, we infused epinephrine (0.06 micrograms X kg-1 min-1) for 90 min into normal humans during combined hepatic vein catheterization and [U-14C]alanine infusion. Epinephrine infusion produced a rise in blood (50-60%) plasma insulin (30-40%), whereas glucagon levels increased only at 30 (19%, P less than 0.05). Net splanchnic output transiently by 150% then returned base line 60 min. In contrast,...
This study aimed to determine the serum levels of free L-carnitine, acetyl-L-carnitine and 34 acyl-L-carnitine in healthy subjects patients with or at risk Alzheimer's disease. Twenty-nine probable disease, 18 mild cognitive impairment amnestic type, 24 subjective memory complaint 46 were enrolled study, carnitine acyl-carnitines measured by tandem mass spectrometry. The concentrations progressively decreased passing from group (mean±SD, 5.6±1.3 μmol/L) (4.3±0.9 μmol/L), (4.0±0.53 up disease...
Adipose tissue is a multifunctional organ involved in many physiological and metabolic processes through the production of adipokines and, particular, adiponectin. Caloric restriction one most important strategies against obesity today. The very low-calorie ketogenic diet (VLCKD) represents type caloric with or extremely low daily food energy consumption. This study aimed to investigate effects VLCKD on anthropometric biochemical parameters such as adiponectin levels, well analyzing...
Exhaled breath condensate (EBC) is a noninvasive method for the study of airway lining fluid. Nuclear magnetic resonance (NMR) spectroscopy can provide biochemical profiles metabolites in biological samples. The aim present was to validate NMR metabonomic analysis EBC adults, assessing role pre-analytical variables (saliva and disinfectant contamination) potential clinical feasibility. In total, 36 paired saliva samples, obtained from healthy subjects, laryngectomised patients chronic...
This study was designed to evaluate the influence of iv infusion (50-1 ng kg-1 min) epinephrine, norepinephrine, and isoproterenol on glucose homeostasis in normal man. Epinephrine produced a 40–45 mg/dl increment plasma concentration which resulted from both transient 85–90% rise production (P <0.005) failure uptake increase consistently response hyperglycemia. Plasma insulin increased slightly (20–25‥), while glucagon remained unchanged. Norepinephrine much smaller (25–30‥) (10–15 mg/dl)...
Element profiling is an interesting approach for understanding neurodegenerative processes, considering that compelling evidences show element toxicity might play a crucial role in the onset and progression of Alzheimer's disease (AD). Aim this study was to profile 22 serum elements subjects with or at risk AD. Thirtyfour patients probable AD, 20 mild cognitive impairment (MCI), 24 subjective memory complaint (SMC) 40 healthy (HS) were included study. Manganese, iron, copper, zinc, selenium,...
Caloric restriction is a valid strategy to reduce the visceral adipose tissue (VAT) content in obese persons. Hypocretin-1 (orexin-A) neuropeptide synthesized lateral hypothalamus that strongly modulates food intake, thus influencing accumulation. Therapeutic diets obesity treatment may combine advantages of caloric and dietary ketosis. The current study aimed evaluate effect very low calorie ketogenic diet (VLCKD) population patients.Adiposity parameters orexin-A serum profiling were...
Abnormalities in the plasma amino acid profile have been reported Alzheimer disease (AD), but no data exist for prodromal phase characterized by subjective memory complaint (SMC). It was our aim to understand if serum levels change along continuum from normal AD, and identify possible diagnostic biomarkers.Serum of 15 acids 2 organic were determined 4 groups participants - 29 with probable 18 mild cognitive impairment (MCI), 24 SMC, 46 cognitively healthy subjects (HS) electrospray tandem...
Molecular imaging of separate but still incompletely resolved spots on high-performance thin-layer chromatography (HPTLC) plates is used for the direct analysis porcine brain lipids by desorption electrospray ionization mass spectrometry (DESI-MS). Eight class-specific were imaged in negative ion mode and shown to contain more than fifty lipids. A low lateral resolution 400 × μm allowed simple, rapid, incomplete separation be combined with DESI identification many components these extremely...
Exhaled breath condensate (EBC) collection is a noninvasive method to investigate lung diseases. EBC usually collected with commercial/custom-made condensers, but the optimal condensing temperature often unknown. As such, physical and chemical properties of exhaled metabolites should be considered when setting temperature, therefore requiring validation standardization collecting procedure. frequently used in nuclear magnetic resonance (NMR)-based metabolomics, which unambiguously recognizes...
Biosensors are easy-to-use and cost-effective devices that emerging as an attractive tool, not only in settling diagnosis or disease monitoring, but also mass screening tests, a timely topic impacts on daily life of the whole society. Nanotechnologies lend themselves to development highly sensitive whose realization has become very interdisciplinary topic. Relying enhancement fluorescence signal detected at surface patterned gold nanoparticles, we report behavior analytical device detecting...
Here we present results from a survey on anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seroprevalence in healthy blood donors low incidence disease 2019 area (Apulia region, South Eastern Italy). Among 904 subjects tested, only nine cases (0.99%) antibodies against SARS-CoV-2 were demonstrated. All the seropositive patients negative for research of viral RNA by reverse transcription polymerase chain reaction nasopharyngeal swabs. These data, along with those recently...
The last ten years have been characterized by an enormous step forward in the therapy and management of patients with Cystic Fibrosis (CF), thanks to development combination Transmembrane Receptor (CFTR) correctors potentiators. Specifically, approved triple elexacaftor/tezacaftor/ivacaftor has demonstrated improve lung function CF both homozygous Phe508del Phe508del/minimal genotypes. Here we assessed effect carrying genotype (n = 20) after one year treatments on liver nutrient absorption a...
Fatty liver disease is a serious complication of childhood obesity. Calorie-restricted regimen (RCR) one the effective therapy for this condition. Aim study was to evaluate effect lycopene-rich tomato sauce with oregano and basil extracts in obese children fatty on RCR. 61 were enrolled, 52 completed study. A randomized cross over clinical trial performed. Participants assigned RCR alone or supplement juice 60 days; subsequently, groups switched alternative next days. Reduction BMI, HOMA-IR,...
Abstract It is still debated whether non-specific preclinical symptoms of Alzheimer’s disease (AD) can have diagnostic relevance. We followed the evolution from cognitively normal to AD by NMR-based metabolomics blood sera. Multivariate statistical analysis NMR profiles yielded models that discriminated subjective memory decline (SMD), mild cognitive impairment (MCI) and AD. validated a panel six statistically significant metabolites predicted SMD, MCI in blind cohort with sensitivity values...
Alzheimer’s disease (AD), a neurodegenerative disorder, is the most common cause of dementia in elderly population. Since its original description, there has been intense debate regarding factors that trigger pathology. It becoming apparent AD more than brain and harms whole-body metabolism. We analyzed 630 polar apolar metabolites blood 20 patients with healthy individuals, to determine whether composition plasma could offer additional indicators evaluate any alterations metabolic pathways...
Cerebral ischemia is a pathological condition characterized by complete blood and oxygen supply deprivation to neuronal tissue. The ischemic brain compensates for the rapid decline in ATP levels increasing anaerobic glycolysis rate, which leads lactate accumulation subsequent acidosis. Astrocytes play critical role regulating cerebral energy metabolism. Mitochondria are significant targets hypoxia-ischemia injury, disruptions mitochondrial homeostasis cellular energetics worsen outcomes,...