A5000253457- Hemoglobin structure and function
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- Protein Structure and Dynamics
- Synthesis and biological activity
- Tryptophan and brain disorders
- Erythrocyte Function and Pathophysiology
- Proteins in Food Systems
- Metal complexes synthesis and properties
- Antioxidant Activity and Oxidative Stress
- Pancreatic function and diabetes
- Protein Hydrolysis and Bioactive Peptides
- Analytical Chemistry and Chromatography
- Neonatal Health and Biochemistry
- Computational Drug Discovery Methods
- Biochemical Analysis and Sensing Techniques
- Biochemical effects in animals
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Microencapsulation and Drying Processes
- Bipolar Disorder and Treatment
- Advanced Glycation End Products research
- vaccines and immunoinformatics approaches
- Organometallic Compounds Synthesis and Characterization
- Drug Transport and Resistance Mechanisms
University of Belgrade
2016-2025
University of Belgrade – Faculty of Chemistry
2016-2025
Institute of Chemistry
2019
Faculty (United Kingdom)
2015
University of Glasgow
1996
Military Medical Academy
1991
Poor bioavailability and reduced stability are the main drawbacks to efficiently utilizing many naturally occurring antioxidants, so their binding circulatory proteins is essential. This work investigated whether major human proteins, besides albumin, including transferrin, alpha-2-macroglobulin, fibrinogen, bind widely consumed antioxidants food supplements, quercetin, trans-resveratrol, dihydrolipoic acid, thus filling gap of detailed pharmacokinetic properties these supplements. Detailed...
C-phycocyanin (C-PC) represents a significant component of the cyanobacteria Arthrospira platensis (Spirulina) biomass. Beyond its nutritional value, this protein exhibits numerous beneficial biological activities. A covalently attached chromophore, phycocyanobilin, gives C-PC blue color, enabling use as natural food colorant. Additionally, phycocyanobilin various bioactive properties, including metal-binding key drawback to broader industrial application is poor stability. Alternative...
PFAS (per- and polyfluorinated alkyl substances) are synthetic compounds prized for their stability across various industries, but they pose an increasing threat to the environment human health. Following regulation of long-chain PFAS, short-chain ultra-short-chain molecules have been introduced as substitutes, yet bioaccumulation potential remains poorly understood. In this study, we combined experimental (intrinsic fluorescence, microscale thermophoresis, clotting assays) computational...
The novel approach in the treatment of complex multifactorial diseases, such as neurodegenerative disorders and cancer, requires a development efficient multi-targeting oriented drugs. Since oxidative stress significantly contributes to pathogenesis cancer disorders, potential drug candidates should possess good antioxidant properties. Due promising biological activities shown for structurally related (1,3-thiazol-2-yl)hydrazones, focused library 12 benzylidene-based...
Phycocyanobilin (PCB) binds with high affinity (2.2 x 106 M-1 at 25°C) to human serum albumin (HSA) sites located in IB and IIA subdomains. The aim of this study was examine effects PCB binding on protein conformation stability. Using 300 ns molecular dynamics (MD) simulations, UV-VIS spectrophotometry, CD, FT-IR, spectrofluorimetry, thermal denaturation susceptibility trypsin digestion, we studied the stability rigidity HSA, as well conformational changes itself upon protein. MD simulation...
The binding of ubiquitous serum ligands (free fatty acids) to human albumin (HSA) or its glycation can affect thiol group reactivity, thus influencing antioxidant activity. effects stearic acid (SA) and glucose on HSA structural changes content reactivity were monitored by fluoroscopy the Ellman method during a 14-day incubation in molar ratios that mimic pathophysiological conditions. Upon with 5 mM glucose, was same as without it, three different HSA:SA (HSA:SA-1:1-2-4). protective effect...
High affinity, stereo-selective binding of phycocyanobilin<italic>M</italic>-conformer to HSA, and its competition with bilirubin, warfarin hemin for the protein.
β-lactoglobulin (BLG) is a major whey protein with numerous techno-functional properties desirable for the food industry. Phycocyanobilin (PCB), bioactive pigment of Arthrospira platensis health-promoting effects, covalently binds to BLG at physiological pH. This study investigated effects this covalent modification on functional properties. The BLG–PCB adduct possesses enhanced antioxidant properties, and bound PCB protects against free radical-induced oxidation. Despite similar thermal...
Objective This study was set out to examine the impact of atypical antipsychotic drugs: aripiprazole, clozapine, ziprasidone, olanzapine, quetiapine, sertindole and amisulpride on activity antioxidant defence enzymes in human erythrocytes vitro . Methods Cu,Zn‐superoxide dismutase (SOD1), catalase (CAT), selenium‐dependent glutathione peroxidase reductase activities were determined after drugs incubation with blood 15 apparently healthy non‐smoking male volunteers (ages 23–39) for 1 h at 37...
Atypical antipsychotics produce severe side effects including myocarditis that may be attributed to oxidative stress. The aim of this study was investigate the influence clozapine, ziprasidone, and sertindole on rat heart morphology determine whether redox imbalane plays a role in development histopathological changes. Adult 3-month-old male Wistar rats were treated with recommended daily dose for selected drugs. After 4 week treatment analysis performed expression activity antioxidant...