A5078341152- Algal biology and biofuel production
- Proteins in Food Systems
- Protein Hydrolysis and Bioactive Peptides
- Hemoglobin structure and function
- Protein Interaction Studies and Fluorescence Analysis
- Neonatal Health and Biochemistry
- Antioxidant Activity and Oxidative Stress
- Advanced Glycation End Products research
- Microencapsulation and Drying Processes
- Tea Polyphenols and Effects
- Muscle metabolism and nutrition
- Seaweed-derived Bioactive Compounds
- Enzyme Structure and Function
- Food Industry and Aquatic Biology
- Enzyme Catalysis and Immobilization
- Liver Disease and Transplantation
- Dye analysis and toxicity
- Photosynthetic Processes and Mechanisms
- Biochemical effects in animals
- Biochemical Analysis and Sensing Techniques
- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Biochemical Acid Research Studies
- Meat and Animal Product Quality
- Liver Disease Diagnosis and Treatment
University of Belgrade
2016-2025
University of Belgrade – Faculty of Chemistry
2016-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2020-2023
CEA Paris-Saclay
2020-2023
Chimie Moléculaire, Macromoléculaire, Matériaux
2022-2023
Laboratoire Léon Brillouin
2020-2022
Sciences et Ingénierie de la Matière Molle
2020-2021
Université Paris-Saclay
2020
Centre National de la Recherche Scientifique
2020
Faculty (United Kingdom)
2015
Poor bioavailability and reduced stability are the main drawbacks to efficiently utilizing many naturally occurring antioxidants, so their binding circulatory proteins is essential. This work investigated whether major human proteins, besides albumin, including transferrin, alpha-2-macroglobulin, fibrinogen, bind widely consumed antioxidants food supplements, quercetin, trans-resveratrol, dihydrolipoic acid, thus filling gap of detailed pharmacokinetic properties these supplements. Detailed...
C-phycocyanin (C-PC) represents a significant component of the cyanobacteria Arthrospira platensis (Spirulina) biomass. Beyond its nutritional value, this protein exhibits numerous beneficial biological activities. A covalently attached chromophore, phycocyanobilin, gives C-PC blue color, enabling use as natural food colorant. Additionally, phycocyanobilin various bioactive properties, including metal-binding key drawback to broader industrial application is poor stability. Alternative...
Phycocyanobilin (PCB) binds with high affinity (2.2 x 106 M-1 at 25°C) to human serum albumin (HSA) sites located in IB and IIA subdomains. The aim of this study was examine effects PCB binding on protein conformation stability. Using 300 ns molecular dynamics (MD) simulations, UV-VIS spectrophotometry, CD, FT-IR, spectrofluorimetry, thermal denaturation susceptibility trypsin digestion, we studied the stability rigidity HSA, as well conformational changes itself upon protein. MD simulation...
High affinity, stereo-selective binding of phycocyanobilin<italic>M</italic>-conformer to HSA, and its competition with bilirubin, warfarin hemin for the protein.
β-lactoglobulin (BLG) is a major whey protein with numerous techno-functional properties desirable for the food industry. Phycocyanobilin (PCB), bioactive pigment of Arthrospira platensis health-promoting effects, covalently binds to BLG at physiological pH. This study investigated effects this covalent modification on functional properties. The BLG–PCB adduct possesses enhanced antioxidant properties, and bound PCB protects against free radical-induced oxidation. Despite similar thermal...
Packed with hemoglobin, an essential protein for oxygen transport, human erythrocytes are a suitable model system testing the pleiotropic effects of lipophilic drugs. Our study investigated interaction between antipsychotic drugs clozapine, ziprasidone, sertindole, and hemoglobin under simulated physiological conditions. Analysis fluorescence quenching at different temperatures data obtained from van't Hoff diagram molecular docking indicate that interactions static tetrameric has one...
Blue C-phycocyanin (C-PC), the major Spirulina protein with innumerable health-promoting benefits, is an attractive colourant and food supplement. A crucial obstacle to its more extensive use relatively low stability. This study aimed screen various food-derived ligands for their ability bind stabilise C-PC, utilising spectroscopic techniques molecular docking. Among twelve examined ligands, fluorescence quenching revealed that only quercetin, coenzyme Q10 resveratrol had a moderate affinity...
The binding of a popular food supplement and well-known antioxidant, dihydro-alpha-lipoic acid (DHLA) to human serum albumin (HSA) was characterised. monitored by several spectroscopic methods together with the molecular docking approach. HSA able bind DHLA moderate affinity, 1.00?0.05?104 M-1. Spectroscopic data demonstrated that preferential site for on is IIA (Sudlow I). Both experimental analysis identified electrostatic (salt bridges) hydrogen bonds as key interactions involved in HSA....
Under simulated physiological conditions, this study investigates the interaction between nutraceutical phycocyanobilin (PCB) and universal anti-protease protein human alpha-2-macroglobulin (α2M). Extensive molecular docking analyses on multiple α2M conformations, spectroscopic techniques, activity assays were utilized to examine complex formation. The results revealed that for every conformation, two high energy binding sites exist: first, conformationally independent, at interface region...