A5080273111- Metal complexes synthesis and properties
- Retinal and Macular Surgery
- Intraocular Surgery and Lenses
- Traumatic Ocular and Foreign Body Injuries
- Ferrocene Chemistry and Applications
- Education, Psychology, and Social Research
- Botany and Plant Ecology Studies
- DNA and Nucleic Acid Chemistry
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Child Abuse and Related Trauma
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
- Retinopathy of Prematurity Studies
- Lichen and fungal ecology
- Plant Pathogens and Resistance
- Peatlands and Wetlands Ecology
- Connective tissue disorders research
- Ocular Diseases and Behçet’s Syndrome
- Peripheral Artery Disease Management
- Bioactive Compounds and Antitumor Agents
- Cancer therapeutics and mechanisms
- Retinal Diseases and Treatments
- Restraint-Related Deaths
- Enzyme function and inhibition
University Hospital in Motol
2017-2023
Charles University
2001-2023
Karlovac University of Applied Sciences
2022
University Hospital Plzen
2021
Palacký University Olomouc
2008-2019
Institute of Molecular and Translational Medicine
2015-2019
University of Hradec Králové
2019
Czech Academy of Sciences, Institute of Psychology
2018
Czech Academy of Sciences
2011-2015
Czech Academy of Sciences, Institute of Biophysics
2008-2015
An iridium(<sc>iii</sc>) complex [(η<sup>5</sup>-C<sub>5</sub>Me<sub>4</sub>C<sub>6</sub>H<sub>4</sub>C<sub>6</sub>H<sub>5</sub>)Ir(phen)Cl]PF<sub>6</sub>(phen = phenanthroline) exhibits dual effects in killing cancer cells causing nuclear DNA damage and mitochondrial dysfunction simultaneously.
Carbonic anhydrase IX (CAIX) is a transmembrane enzyme that regulates pH in hypoxic tumors and promotes tumor cell survival. Its expression associated with the occurrence of metastases poor prognosis. Here, we present nine derivatives cobalt bis(dicarbollide)(1-) anion substituted at boron or carbon sites by alkysulfamide group(s) as highly specific selective inhibitors CAIX. Interactions these compounds active site CAIX were explored on atomic level using protein crystallography. Two...
The synthesis and characterization of ruthenium(II) arene complexes [(η6-arene)Ru(N,N)Cl]0/+, where N,N = 2,2′-bipyridine (bipy), 2,2′-bipyridine-3,3′-diol (bipy(OH)2) or deprotonated (bipy(OH)O) as N,N-chelating ligand, benzene (bz), indan (ind), biphenyl (bip), p-terphenyl (p-terp), tetrahydronaphthalene (thn), tetrahydroanthracene (tha) dihydroanthracene (dha), are reported, including the X-ray crystal structures [(η6-tha)Ru(bipy)Cl][PF6] (1), [(η6-tha)Ru(bipy(OH)O)Cl] (2)...
The PtIV diazido complex trans,trans,trans-[Pt(N3)2(OH)2(pyridine)2] (1) is unreactive in the dark but cytotoxic when photoactivated by UVA and visible light. We have shown that 1 accumulates tumor cells binds strongly to nuclear DNA under conditions which it toxic cells. nature of adducts, including conformational alterations, induced are distinctly different from those produced conventional cisplatin or transplatin. In addition, observation major adducts able efficiently stall RNA...
A combination of biophysical, biochemical, and computational techniques was used to delineate mechanistic differences between the platinum–acridine hybrid agent [PtCl(en)(L)](NO3)2 (complex 1, en = ethane-1,2-diamine, L 1-[2-(acridin-9-ylamino)ethyl]-1,3-dimethylthiourea) a considerably more potent second-generation analogue containing L′ N-[2-(acridin-9-ylamino)ethyl]-N-methylpropionamidine 2). Calculations at density functional theory level provide rationale for binding preference both...
The present paper focuses on the epipelic cyanobacteria and algae (particularly desmids).Altogether 45 sediment samples were taken at ponds covering a pH/conductivity trophic gradients.Statistic evaluation based environmental variables measured, divided localities into four major groups differing also by quality its algal flora.Altogether 39 cyanobacterial species found including sedimented planktic or litoral forms, with prevalence of motile filamentous genera (Komvophoron, Oscillatoria,...
Abstract We report the clinical findings of 26 individuals from 16 unrelated families carrying variants in COL2A1 or COL11A1 genes. Using Sanger and next‐generation sequencing, 11 different (seven novel), were identified 13 (19 affected individuals), all diagnosed with Stickler syndrome (STL) type 1. In nine families, disease‐causing variant arose de novo. Phenotypically, we observed myopia (95%) retinal detachment (47%), joint hyperflexibility (92%), midface retrusion (84%), cleft palate...
Nuclear factor-kappa B (NF-кB) comprises a family of protein transcription factors that have regulatory function in numerous cellular processes and are implicated the cancer cell response to antineoplastic drugs, including cisplatin. We characterized effects DNA adducts cisplatin ineffective transplatin on affinity NF-кB proteins their consensus sequence (кB site). Although кB site-NF-κB interaction was significantly perturbed by cisplatin, were markedly less effective both cell-free media...
In this study, we have compared four 68Ga-labeled peptides (three Arg-Gly-Asp (RGD) and substance-P) with two 18F-tracers clinically approved for tumor imaging. We studied in vitro vivo characteristics of selected radiolabeled tracers a glioblastoma multiforme model. The part the study was mainly focused on evaluation radiotracers stability under various conditions. also determined 68Ga-radiotracers by analysis murine urine collected at time points after injection. behavior tested...
The seasonal succession of algal species in the epipelon shallow, mesotrophic pond Bezedník (Czech Republic) with special attention to diatoms (particularly Sellaphora) is described.The composition epipelic assemblages changed over time both higher taxonomic groupings and representations.Spring autumn were characterized by a dominance diatoms; euglenophytes had their maximum June cyanobacteria typical for summer season.The occurrence also correlated water temperature.However, although growth...
Flubendazole (FLU), an anthelmintic drug of benzimidazole type, is now considered a promising anti-cancer agent due to its tubulin binding ability and low system toxicity. The present study was aimed at determining more information about FLU reduction in human liver, because this has been insufficient until now. Subcellular fractions from the liver 12 patients (6 male 6 female patients) were used stereospecificity, cellular localization, coenzyme preference, enzyme kinetics, possible...
The first objective of our study was to determine the radiation exposure received by patients during tin-filtrated ultra-low-dose computed tomography (TFULDCT) sacroiliac joints and compare those conventional X-ray doses. For comparison, we added a cohort examined low-dose CT (LDCT) without tin filtration. second results TFULDCT in detection sacroiliitis.Our retrospective covered 45 patients, who were for suspected axial spondyloarthritis (AxSpA). group underwent as well radiography (CR);...
Purpose: To evaluate the efficacy of pars plana vitrectomy (PPV) as an anti-inflammatory therapy in pediatric recurrent intermediate uveitis. Methods: A retrospective study evaluated long-term results PPV indicated for uveitis with a mean observation period 10.3 years (range 7-15.6 years) 6 children (mean age 8 years, range 6-12 years). Pars was performed on 10 eyes standard manner and initiated by vitreous sampling laboratory examination. Data recorded were perioperative or postoperative...