A5073365495- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Asthma and respiratory diseases
- Ion channel regulation and function
- Force Microscopy Techniques and Applications
- Neonatal Respiratory Health Research
- Cardiomyopathy and Myosin Studies
- Advanced machining processes and optimization
- Cancer Research and Treatments
- Tracheal and airway disorders
- Alcohol Consumption and Health Effects
- Microfluidic and Bio-sensing Technologies
- Orthopaedic implants and arthroplasty
- Cardiovascular Effects of Exercise
- Muscle Physiology and Disorders
- Mathematical Biology Tumor Growth
- Exercise and Physiological Responses
- Extracellular vesicles in disease
- Advanced Fiber Optic Sensors
- Blood properties and coagulation
- Biometric Identification and Security
- Advanced Surface Polishing Techniques
- Hemoglobin structure and function
- Bone Tissue Engineering Materials
Harvard University
2015-2022
University of Calgary
2015-2018
Chongqing University
2011-2014
St. Paul's Hospital
2014
University of British Columbia
2013-2014
Changzhou University
2012-2014
During acute bronchoconstriction, the airway epithelium becomes mechanically compressed, as smooth muscle contracts and narrows. This mechanical compression activates to promote asthmatic remodeling. However, whether compressed can feed back on cause of bronchoconstriction has remained an open question. Here we examine potential for epithelial augment proliferation contraction muscle, thus potentiate further compression. Well-differentiated primary human bronchial (HBE) cells maintained in...
Smooth muscle contraction can be divided into two phases: the initial determines amount of developed force and second phase how well is maintained. The primarily due to activation actomyosin interaction relatively understood, whereas remains poorly understood. Force maintenance in sustained disrupted by strains applied muscle; strain causes cross-bridges detach also cytoskeletal structure disassemble a process known as fluidization, for which underlying mechanism largely unknown. In present...
With every deep inspiration (DI) or sigh, the airway wall stretches, as do smooth muscle cells in wall. In response, cell undergoes rapid stretch-induced cytoskeletal fluidization. As a molecular mechanism underlying fluidization we demonstrate key role for actin-severing protein cofilin. Using primary human cells, simulated DI by imposing transient stretch of physiological magnitude and duration. We used traction microscopy to measure resulting changes contractile forces. After stretch,...
Myosin molecules from smooth muscle and non-muscle cells are known to self-assemble into side-polar filaments in vitro. However, the situ mechanism of filament assembly is not clear question whether there a unique length for myosin still under debate. In this study we measured lengths 16,587 three types using serial electron microscopy (EM). Sheep airway pulmonary arterial as well rabbit carotid were fixed EM ultra-thin (50-60 nm) sections obtained. traced consecutive determine their...
To investigate the effect of total anthraquinone in Cassiae Semen on lipid peroxidation and peroxisome proliferator activated receptors gamma (PPAR-gamma) expression liver tissues rats with alcoholic fatty liver.Referring to literature, it was established animal models feeding alcohol. Rats were randomized into 6 groups, except normal group, other 5 groups had been administered alcohol two times a day for 3 months. killed at end this experiment. It respectively measured that contents ALT,...
The structurally dynamic cytoskeleton is important in many cell functions. Large gaps still exist our knowledge regarding what regulates cytoskeletal dynamics and underlies the structural plasticity. Because Rho-kinase an upstream regulator of signaling events leading to phosphorylation proteins types, we have chosen this kinase as focus present study. In detergent skinned tracheal smooth muscle preparations, quantified eluted from cells over time monitored muscle's ability respond...
Loss of function the tumor suppressor p53 is known to increase rate migration cells transiting narrow pores traditional Boyden chamber assay. Here by contrast we investigate how impacts cellular within a 2D confluent cell layer and 3D collagen-embedded multicellular spheroid. We use two human carcinoma lines, bladder EJ colorectal HCT116. In 2-D layer, for both HCT migratory speeds effective diffusion coefficients null were significantly smaller than in p53-expressing cells. Compared...
At the edge of a confluent cell layer, cell-free empty space is cue that can drive directed collective cellular migration. Similarly, contact guidance also robust mechanical However, it unclear which two effects stronger, and how each mechanism affects To address this question, here we explore trajectories cells migrating collectively on substrate containing micropatterned grooves (10-20 μm in periodicity, 2 height) compared with unpatterned control substrates. Compared controls, substrates...
Airway smooth muscle (ASM) in vivo is constantly subjected to oscillatory strain due tidal breathing and deep inspirations. ASM contractility known be adversely affected by strains, especially those of large amplitudes. Based on the cross-bridge model contraction, it likely that impairs force generation disrupting actomyosin interaction. There also evidence modulates stiffness through induction cytoskeletal remodeling. However, molecular mechanism which alters function not entirely clear....
CCAAT/Enhancer Binding Protein D (CEBPD), a pleiotropic glucocorticoid-responsive transcription factor, modulates inflammatory responses. Of relevance to asthma, expression of CEBPD in airway smooth muscle (ASM) increases with glucocorticoid exposure. We sought characterize CEBPD-mediated transcriptomic responses exposure ASM by measuring changes observed after knockdown and its impact on asthma-related function. Primary cells derived from four donors were transfected or non-targeting (NT)...
Titanium (Ti) is widely used for making tissue engineering implants, due to its good corrosion resistance, biocompatibility and mechanical properties. However, bare Ti does not integrate well with natural bone releases ions particles that are harmful the extracellular matrix. To overcome these problems, substrate could be coated various biocompatible materials including a composite of gelatin hydroxyapatite (HA). few have characterized evaluated coating gelatin/HA on effect related cells. In...
Summary Loss of function the tumor suppressor p53 is generally thought to increase cell motility and invasiveness. Using 2-D confluent 3-D spheroidal assays with bladder carcinoma cells colorectal cells, we report, contrary, that loss can decrease invasion. Abstract For migration single studied in isolation, motility. Here by contrast used layer multicellular spheroid investigate how impacts dissemination invasion cellular collectives. We two human lines, EJ HCT116. began replicating...