A5087503947- Peroxisome Proliferator-Activated Receptors
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Signaling Pathways in Disease
- Supramolecular Self-Assembly in Materials
- Electron and X-Ray Spectroscopy Techniques
- Metal-Organic Frameworks: Synthesis and Applications
- Advanced Electron Microscopy Techniques and Applications
- Supramolecular Chemistry and Complexes
- Pediatric Urology and Nephrology Studies
- DNA and Nucleic Acid Chemistry
- Dark Matter and Cosmic Phenomena
- Plasmonic and Surface Plasmon Research
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Modular Robots and Swarm Intelligence
Dana-Farber Cancer Institute
2022-2023
Harvard University
2022-2023
Max Planck Institute of Molecular Physiology
2017-2023
University of Münster
2022-2023
Abstract Selecting particles from digital micrographs is an essential step in single-particle electron cryomicroscopy (cryo-EM). As manual selection of complete datasets—typically comprising thousands particles—is a tedious and time-consuming process, numerous automatic particle pickers have been developed. However, non-ideal datasets pose challenge to picking. Here we present the picking software crYOLO which based on deep-learning object detection system You Only Look Once (YOLO). After...
Abstract The self-organizational properties of DNA have been used to realize synthetic hosts for protein encapsulation. However, current strategies DNA–protein conjugation still limit true emulation natural host–guest systems, whose formation relies on non-covalent bonds between geometrically matching interfaces. Here we report one the largest complexes semisynthetic origin held in place exclusively by spatially defined supramolecular interactions. Our approach is based decoration inner...
Self-assembled, porous coordination cages with a functional interior find application in controlled guest inclusion/release, drug delivery, separation processes, and catalysis. However, only few studies exist that describe their utilization for the development of self-assembled materials based on 3-dimensional shape external functionalization. Here, dodecyl chain-containing, acridone-based ligands (LA) shape-complementary phenanthrene-derived (LB) are shown to self-assemble heteroleptic...
The elastic features of protein filaments are encoded in their component units and the way they connected, thus defining a biunivocal relationship between monomer result its self-assembly. Using DNA origami approaches, we constructed reconfigurable module, composed two quasi-independent domains four possible interfaces, capable facial lateral growing through specific recognition patterns. Whereas flexibility intra-domains region can be regulated by switchable motifs, inter-domain interfaces...
Import of yeast peroxisomal matrix proteins is initiated by cytosolic receptors, which specifically recognize and bind the respective cargo proteins. At membrane, cargo-loaded receptor interacts with docking protein Pex14p that tightly associated Pex17p. Previous data suggest this interaction triggers formation an import pore for further translocation cargo. The mechanistic principles, however, are unclear, mainly because structures higher-order assemblies still lacking. Here, using...
Abstract Selecting particles from digital micrographs is an essential step in single particle electron cryomicroscopy (cryo-EM). Since manual selection of complete datasets typically comprising many thousands a tedious and time-consuming process, automatic pickers have been developed the past few decades. However, non-ideal pose challenge to picking. Here, we present novel automated picking software called crYOLO, which based on deep learning object detection system “You Only Look Once”...
The double-ring AAA+ ATPase Pex1/Pex6 is required for peroxisomal receptor recycling and essential peroxisome formation. mutations cause severe associated developmental disorders. Despite its pathophysiological importance, mechanistic details of the heterohexamer are not yet available. Here, we report cryoEM structures from Saccharomyces cerevisiae, with an endogenous protein substrate trapped in central pore catalytically active second ring (D2). Pairs Pex1/Pex6(D2) subdomains engage via a...
Cysteine can be synthesized by tRNA-dependent mechanism using a two-step indirect pathway, where O-phosphoseryl-tRNA synthetase (SepRS) catalyzes the ligation of mismatching O-phosphoserine (Sep) to tRNACys followed conversion tRNA-bounded Sep into cysteine Sep-tRNA:Cys-tRNA synthase (SepCysS). In ancestral methanogens, third protein SepCysE forms bridge between two enzymes create ternary complex named transsulfursome. By combination X-ray crystallography, SAXS and EM, together with...
Metallo-supramolecular self-assembly has yielded a plethora of discrete nanosystems, many which show competence in capturing guests and catalyzing chemical reactions. However, the potential low-molecular bottom-up self-assemblies development structured inorganic materials rarely been methodically explored so far. Herein, we present new type metallo-supramolecular surfactant with ability to stabilize non-aqueous emulsions for significant period. The molecular design is based on heteroleptic...
Oxalyl-CoA synthetase from Saccharomyces cerevisiae is one of the most abundant peroxisomal proteins in yeast and hence has become a model to study translocation. It contains C-terminal Peroxisome Targeting Signal 1, which however partly dispensable, suggesting additional receptor bindings sites. To unravel any features that may contribute its capacity be recognized as target, we determined assembly overall architecture by an integrated structural biology approach, including X-ray...
Abstract Import of yeast peroxisomal matrix proteins is initiated by cytosolic receptors, which specifically recognize and bind the respective cargo proteins. At membrane, cargo-loaded receptor interacts with docking protein Pex14p that tightly associated Pex17p. Previous data suggest this interaction triggers formation an import pore for further translocation cargo. The mechanistic principles are however unclear, mainly because structures higher order assemblies still lacking. Here, using...
ABSTRACT DNA origami has enabled the development of responsive drug-delivery vehicles with precision features that were previously not attainable in bionanotechnology. To reduce costs creating therapeutics-scale amounts need to bear costly modifications high occupancy, we reused excess staple oligonucleotides are leftover from folding process fold additional origami. We determined a can be successfully folded up 80% cost savings by cyclic recovery and reuse strands. found evidence higher...
DNA origami is a popular nanofabrication strategy that employs self-assembly of long single scaffold strand, typically less than 10 kilobases in length, with hundreds shorter staple strands into desired shape. In particular, arranged as single-layer rectangle has proven flat pegboards can display functionalities at staple-strand breakpoints, off the sides constituent double helices, ∼5.3 nm rhombic-lattice spacing. For applications demand tighter spacing, be displayed instead on termini...
ABSTRACT Oxalyl-CoA synthetase from Saccharomyces cerevisiae is one of the most abundant peroxisomal protein in yeast and hence has become a model to study translocation. It contains C-terminal Peroxisome Targeting Signal 1, which however partly dispensable, suggesting additional receptor bindings sites. To unravel any features that may contribute its capacity be recognized as target, we determined assembly overall architecture by an integrated structural biology approach, including X-ray...
Abstract The double-ring AAA+ ATPase Pex1/Pex6 is required for peroxisomal receptor recycling and essential peroxisome formation. mutations cause severe associated developmental disorders. Despite its pathophysiological importance, mechanistic details of the heterohexamer are not yet available. Here, we report cryoEM structures from Saccharomyces cerevisiae , with an endogenous protein substrate trapped in central pore second ring (D2). Pairs Pex1/Pex6(D2) subdomains engage via a staircase...