A5080348355- Reproductive tract infections research
- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Clostridium difficile and Clostridium perfringens research
- Herpesvirus Infections and Treatments
- Urinary Tract Infections Management
- Syphilis Diagnosis and Treatment
- Insect and Pesticide Research
- Bacteriophages and microbial interactions
- Cervical Cancer and HPV Research
- Genital Health and Disease
- Toxin Mechanisms and Immunotoxins
- Health and Medical Studies
- Advanced Electron Microscopy Techniques and Applications
- Bacillus and Francisella bacterial research
- Electron and X-Ray Spectroscopy Techniques
- Infectious Diseases and Mycology
- Monoclonal and Polyclonal Antibodies Research
- Streptococcal Infections and Treatments
- Gout, Hyperuricemia, Uric Acid
- Entomopathogenic Microorganisms in Pest Control
- Mycobacterium research and diagnosis
- Rabies epidemiology and control
- Plant and fungal interactions
- Cancer Research and Treatments
Max Planck Institute of Molecular Physiology
2018-2021
Friedrich-Loeffler-Institut
2007-2018
Organisation Mondiale de la Santé Animale
2013
Universität Hamburg
2003
Leibniz Institute of Virology (LIV)
2003
Federal Institute for Health Protection of Consumers and Veterinary Medicine
1998
Friedrich-Alexander-Universität Erlangen-Nürnberg
1998
Institute of Molecular Biology
1985
Internationale Akademie Berlin
1977
Abstract Selecting particles from digital micrographs is an essential step in single-particle electron cryomicroscopy (cryo-EM). As manual selection of complete datasets—typically comprising thousands particles—is a tedious and time-consuming process, numerous automatic particle pickers have been developed. However, non-ideal datasets pose challenge to picking. Here we present the picking software crYOLO which based on deep-learning object detection system You Only Look Once (YOLO). After...
Summary In 2005, an outbreak of severe respiratory disease in a mixed poultry flock that was infected with Chlamydophila ( C. ) psittaci led to dissemination the infection at least 100 small farms 11 districts Central Germany. At same time, total 24 persons contact from one flocks reported flu‐like symptoms their physician, thus suggesting zoonotic transmission. Within 3 weeks, seven individuals had be hospitalized, three them requiring intensive care. Analysis omp A sequences chlamydial...
The currently used genotyping system for the avian zoonotic pathogen Chlamydophila (C.) psittaci has evolved from serology and is based on ompA sequence variations. It includes seven two non-avian genotypes. Restriction enzyme cleavage of amplified gene and, less frequently, sequencing are being examination, but, beside methodological limitations, an increasing number recently tested strains could not be assigned to any established genotype. Comprehensive analysis all available sequences...
ABSTRACT The obligatory intracellular bacterium Chlamydophila psittaci is the causative agent of psittacosis in birds and humans. capability this zoonotic pathogen to develop a persistent phase likely play role chronicity infections, as well failure antibiotic therapy immunoprophylaxis. To elucidate three different vitro models for transition C. persistence (iron depletion, penicillin G treatment, gamma interferon [IFN-γ] exposure), set 27 genes was examined by mRNA expression analysis using...
Chlamydia (C.) psittaci is the causative agent of psittacosis, a zoonotic disease in birds and man. In addition, C. has been repeatedly found domestic animals is, at least calves, also able to induce respiratory disease. Knowledge about transmission routes cattle herds still deficient, nothing known differences host response after either experimental or natural exposure psittaci. Therefore, our recently developed infection model was exploited evaluate (i) presence pathogen blood, excretions...
This study aimed to establish and evaluate a bovine respiratory model of experimentally induced acute C. psittaci infection. Calves are natural hosts pathogenesis may resemble the situation in humans. Intrabronchial inoculation strain DC15 was performed calves aged 2–3 months via bronchoscope at four different challenge doses from 106 109 inclusion-forming units (ifu) per animal. Control groups received either UV-inactivated or cell culture medium. While ifu/calf resulted mild infection...
Serological analysis of Chlamydia (C.) spp. infections is still mainly based on micro-immunofluorescence and ELISA. To overcome the limitations conventional serology, we have designed a novel microarray carrying 52 synthetic peptides representing B-cell epitopes from immunodominant proteins all 11 chlamydial species. The new assay has been validated using monospecific mouse hyperimmune sera. Subsequently, serum samples cattle, sheep humans with known history infection were examined. For...
Abstract Tc toxins are bacterial protein complexes that inject cytotoxic enzymes into target cells using a syringe-like mechanism. composed of membrane translocator and cocoon encapsulates toxic enzyme. The enzyme varies between from different species is not conserved. Here, we investigate whether the can be replaced by other small proteins origin properties, namely Cdc42, herpes simplex virus ICP47, Arabidopsis thaliana iLOV, Escherichia coli DHFR, Ras-binding domain CRAF kinase, TEV...
Mycoplasmas comprise a conglomerate of pathogens and commensals occurring in humans animals. The genus Mycoplasma alone contains more than 120 species at present, new members are continuously being discovered. Therefore, it seems promising to use single highly parallel detection assay rather develop separate tests for each individual species. In this study, we have designed DNA microarray carrying 70 oligonucleotide probes derived from the 23S rRNA gene 86 tuf target regions. Following PCR...
α-Xenorhabdolysins (Xax) are α-pore-forming toxins (α-PFT) that form 1–1.3 MDa large pore complexes to perforate the host cell membrane. PFTs used by a variety of bacterial pathogens attack cells. Due lack structural information, molecular mechanism action Xax is poorly understood. Here, we report cryo-EM structure XaxAB complex from Xenorhabdus nematophila and crystal structures soluble monomers XaxA XaxB. The reveal XaxB built similarly appear as heterodimers in 12–15 subunits containing...
In a previous study, increased weight of the adrenal glands was found in small group persons who committed violent suicides. This finding confirmed our which comprised 42 suicide cases and 37 control cases. Further analysis with special consideration toward "relative weight" (weight/body surface) revealed that relative combined >6 g/m2 may be morphologic sign depressive disorder prior to death if no other disease known effect on adrenals is present. These results are consistent clinical...
Vesicle formation at endomembranes requires the selective concentration of cargo by coat proteins. Conserved adapter protein complexes Golgi (AP-3), endosome (AP-1), or plasma membrane (AP-2) with their conserved core domain and flexible ear domains mediate this function. These also rely on small GTPase Arf1 and/or specific phosphoinositides for binding. The structural details that influence these processes, however, are still poorly understood. Here we present cryo-EM structures full-length...
Abstract Background To study the specific antibody response to infection with Mycoplasma mycoides subsp. Small Colony ( Mmm SC), agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels sera collected during experimental trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, competitive ELISA (cELISA) and immunoblotting (IBT). In addition, lung tissue samples were by culture. Results A total 89% (51/59)...