A5000674264- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- RNA Research and Splicing
- Plant Virus Research Studies
- Plant pathogens and resistance mechanisms
- RNA modifications and cancer
- Biotin and Related Studies
- Peptidase Inhibition and Analysis
- bioluminescence and chemiluminescence research
- Mycotoxins in Agriculture and Food
- Plant Stress Responses and Tolerance
- Plant Pathogens and Fungal Diseases
- Greenhouse Technology and Climate Control
- RNA regulation and disease
- Vitamin D Research Studies
- Fungal Plant Pathogen Control
- Toxin Mechanisms and Immunotoxins
- Immunodeficiency and Autoimmune Disorders
- Plant Molecular Biology Research
- Clostridium difficile and Clostridium perfringens research
- Genomics and Chromatin Dynamics
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
Hospital for Sick Children
2023-2025
University of California, Berkeley
2012-2025
Plant Gene Expression Center
2025
United States Department of Agriculture
2025
Agricultural Research Service
2025
University of Toronto
2008-2012
Plant Biotechnology Institute
2006
National Research Council Canada
2006
The self-association of Toll/interleukin-1 receptor/resistance protein (TIR) domains has been implicated in signaling plant and animal immunity receptors. Structure-based studies identified different TIR-domain dimerization interfaces required for the nucleotide-binding oligomerization domain-like receptors (NLRs) L6 from flax disease resistance RPS4 Arabidopsis Here we show that crystal structure TIR domain NLR suppressor npr1-1, constitutive 1 (SNC1) contains both an L6-like interface...
Plants depend on innate immunity to prevent disease. Plant pathogenic bacteria, like Pseudomonas syringae and Xanthomonas campestris, use the type III secretion system as a molecular syringe inject secreted effector (T3SE) proteins in plants. The primary function of most T3SEs is suppress immunity; however, plant can evolve nucleotide-binding domain-leucine-rich repeat domain-containing recognize specific T3SEs. AtZAR1 NLR induces strong defense responses against P. X. campestris T3SE HopZ1a...
Upon recognition of pathogen virulence effectors, plant nucleotide-binding leucine-rich repeat (NLR) proteins induce defense responses including localized host cell death. In an effort to understand the molecular mechanisms leading this response, we examined Arabidopsis thaliana NLR protein RECOGNITION OF PERONOSPORA PARASITICA1 (RPP1), which recognizes Hyaloperonospora arabidopsidis effector ARABIDOPSIS THALIANA RECOGNIZED1 (ATR1). Expression N-terminus RPP1, Toll/interleukin-1 receptor...
Proximity-dependent biotinylation (PDB) techniques provide information about the molecular neighborhood of a protein interest, yielding insights into its function and localization. Here, we assessed how different labeling enzymes streptavidin resins influence PDB results. We compared high-confidence interactors DNA/RNA-binding transactive response DNA-binding 43 kDa (TDP-43) identified using either miniTurbo (biotin ligase) or APEX2 (peroxidase) enzymes. also evaluated two commercial...
Despite the tremendous economic impact of cereal crop pathogens such as fungus Fusarium graminearum, development strategies for enhanced protection is hampered by complex host genetics and difficulties in performing high-throughput analyses. To bypass these challenges, we have developed an assay which interaction between F. graminearum model plant Arabidopsis thaliana monitored liquid media 96-well plates. In this assay, fungal infection associated with dark lesion-like spots on cotyledons...
Many bacterial toxins exert their cytotoxic effects by enzymatically inactivating one or more cytosolic targets in host cells. To reach intracellular targets, these possess functional domains subdomains that interact with and exploit various factors biological processes. Despite great progress identifying many of the key involved uptake toxins, significant knowledge gaps remain as to how partially characterized newly discovered microbial processes intoxicate target Proximity-dependent...
Pseudomonas syringae pv. tomato DC3000 ( Pst DC3000) is a plant pathogenic bacterium that possesses complicated motility regulation pathways including typical chemotaxis system. A significant portion of our understanding about the genes functioning in based on comparison to other bacteria. This leaves uncertainty whether gene functions are conserved, especially since specific regulatory modules can have opposite sets . In this study, we used competitive selection enrich for mutants with...
ABSTRACT Living cells adapt to stress by protecting essential resources, such as stabilizing transcripts. Stress granules, which form during through the coalescence of polyadenylated transcripts released from polysomes and RNA-binding proteins, are implicated in post-transcriptional regulation, although their precise roles remain contested. To address this, we generated dynamic proteomic landscapes granules assembly disassembly under oxidative hyperosmotic using multi-bait BioID profiling...
Summary The study of plant pathogenesis and the development effective treatments to protect plants from diseases could be greatly facilitated by a high‐throughput pathosystem evaluate small‐molecule libraries for inhibitors pathogen virulence. interaction between Gram‐negative bacterium Pseudomonas syringae Arabidopsis thaliana is model pathogenesis. However, robust assay score outcome this currently lacking. We demonstrate that seedlings incubated with P. in liquid culture display...
The outcome of plant-pathogen interactions is influenced significantly by endogenous small molecules that coordinate plant defence responses. There currently tremendous scientific and commercial interest in identifying chemicals whose exogenous application activates defences affords protection from pathogen infection. In this review, we provide a survey compounds known to induce disease resistance plants, with particular emphasis on how each compound was originally identified, its putative...
Gram-negative bacterial pathogens have evolved a number of virulence-promoting strategies including the production extracellular polysaccharides such as alginate and injection effector proteins into host cells. The induction these virulence mechanisms can be associated with concomitant downregulation abundance that trigger immune system, flagellin. In Pseudomonas syringae, we observed motility flagellin were significantly reduced under conditions induce type III secretion system. To identify...
NLR (nucleotide-binding [NB] leucine-rich repeat [LRR] receptor) proteins are critical for inducing immune responses in response to pathogen proteins, and must be tightly modulated prevent spurious activation the absence of a pathogen. The ZAR1 recognizes diverse effector from Pseudomonas syringae, including HopZ1a, Xanthomonas species. Receptor-like cytoplasmic kinases (RLCKs) such as ZED1, interact with provide specificity different HopZ1a. We previously developed transient expression...
Abstract Background The sulfanilamide family comprises a clinically important group of antimicrobial compounds which also display bioactivity in plants. While there is evidence that sulfanilamides inhibit folate biosynthesis both bacteria and plants, the complete network plant responses to these remains be characterized. As such, we initiated two forward genetic screens Arabidopsis order identify mutants exhibit altered sensitivity compounds. These were based on growth phenotype seedlings...
Pathogens secrete effector proteins into host cells to suppress immunity and promote pathogen virulence, although many features at the molecular interface of host-pathogen interactions remain be characterized. In a yeast two-hybrid assay, we found that Pseudomonas syringae HopZ1a interacts with Arabidopsis transcriptional regulator Abscisic Acid Repressor 1 (ABR1). Further analysis revealed ABR1 multiple P. effectors, suggesting it may targeted as susceptibility hub. Indeed, loss-of-function...
Successful pathogenesis requires a number of coordinated processes whose genetic bases remain to be fully characterized. We utilized high-throughput, liquid media-based assay screen transposon disruptants the phytopathogen Pseudomonas syringae pv. maculicola ES4326 identify genes required for virulence on Arabidopsis. Many identified through this were involved in such as type III secretion, periplasmic glucan biosynthesis, flagellar motility, and amino acid biosynthesis. A small set did not...
Phytopathogens use secreted effector proteins to suppress host immunity and promote pathogen virulence, there is increasing evidence that the host-pathogen interactome comprises a complex network. To identify novel interactors of Pseudomonas syringae HopZ1a, we performed yeast two-hybrid screen identified previously uncharacterized Arabidopsis protein designate HopZ1a interactor 1 (ZIN1). Additional analyses in planta revealed ZIN1 also interacts with several other P. effectors. We show an...
ABSTRACT NLR (Nucleotide-binding [NB] Leucine-rich repeat [LRR] Receptor) proteins are critical for inducing immune responses in response to pathogen proteins, and must be tightly regulated prevent spurious activation the absence of a pathogen. The ZAR1 recognizes diverse effector from Pseudomonas syringae , including HopZ1a, Xanthomonas species. Receptor-like cytoplasmic kinases (RLCKs) such as ZED1, interact with provide specificity different HopZ1a. We previously developed transient...