A5002629678- Helminth infection and control
- Wound Healing and Treatments
- Nanocluster Synthesis and Applications
- Advanced oxidation water treatment
- Nanoparticle-Based Drug Delivery
- Graphene and Nanomaterials Applications
- Microtubule and mitosis dynamics
- Carbon and Quantum Dots Applications
- Autophagy in Disease and Therapy
- Electrochemical Analysis and Applications
- Parasites and Host Interactions
- Cell death mechanisms and regulation
- Nanoparticles: synthesis and applications
- Parasite Biology and Host Interactions
- Diabetic Foot Ulcer Assessment and Management
- Advanced Nanomaterials in Catalysis
- Medicinal Plant Research
- Water Treatment and Disinfection
- COVID-19 Pandemic Impacts
- Advanced Drug Delivery Systems
- Gold and Silver Nanoparticles Synthesis and Applications
- COVID-19 epidemiological studies
- Advanced biosensing and bioanalysis techniques
- Ginger and Zingiberaceae research
- Inflammasome and immune disorders
Thapar Institute of Engineering & Technology
2016-2025
Virginia Tech
2020-2024
Government Medical College
2021
Cedars-Sinai Medical Center
2014-2020
University of Calcutta
2009-2020
Kerala Veterinary and Animal Sciences University
2020
In-Q-Tel
2012-2014
Indian Institute of Science Bangalore
1989
The effect of gold nanoparticles (AuNPs) on the polymerization tubulin has not been examined till now. We report that interaction weakly protected AuNPs with microtubules (MTs) could cause inhibition and aggregation in cell free system. estimate single citrate capped ∼105 heterodimers. Investigation nature by Raman Fourier transform-infrared (FTIR) spectroscopies indicated partial conformational changes microtubules, thus revealing AuNP-induced change is driving force behind observed...
Abstract Periodontal diseases can lead to chronic inflammation affecting the integrity of tooth supporting tissues. Recently, a striking association has been made between periodontal and primary cancers in absence mechanistic understanding. Here we address effect (PI) on tumor progression, metastasis, possible underlining mechanisms. We show that an experimental model PI mice promote lymph node (LN) micrometastasis, as well head neck metastasis 4T1 breast cancer cells, both early late stages...
Lactoferrin (LF) is a non-heme iron-binding glycoprotein involved in the transport of iron blood plasma. In addition, it has many biological functions, including antibacterial, antiviral, antimicrobial, antiparasitic, and, importantly, antitumor properties. this study, we have investigated potential employing lactoferrin-iron oxide nanoparticles (LF-IONPs) as treatment modality for gastric cancer. The study confirms formation LF-IONPs with spherical shape and an average size 5 ± 2 nm,...
The potential roles of insulin, silver, copper and quercetin as antibacterial agents, ROS scavengers cell growth migration promotors, which eventually lead to effective efficient wound healing, are depicted.
Head and neck cancers are often diagnosed at later stages with poor outcomes. Mesenchymal stem cells (MSC) recruited to primary tumor sites where they can have pro- antitumorigenic influence. In trying better understand the dynamics between MSC cancer cells, we found that head cancer-MSC exposure resulted in mesenchymal features, elevated proliferation rate, were more motile, like same fused MSC. We orthotopically grafted parental those MSC, or exposed into tongues of mice. The originally...
Diabetic wounds represent a major issue in medical care and need advanced therapeutic tissue imaging systems for better management. The utilization of nano-formulations involving proteins like insulin metal ions plays significant roles controlling wound outcomes by decreasing inflammation or reducing microbial load. This work reports the easy one-pot synthesis extremely stable, biocompatible, highly fluorescent insulin-cobalt core-shell nanoparticles (ICoNPs) with enhanced quantum yield...
Parabenzoquinone (1,4-benzoquinone) (PBQ) is a bioactve quinone present in cigarette smoke and diesel smoke, which causes severe genotoxic effects both vitro vivo. In the previous study, we showed that microtubules are one of major targets smoke-induced damage lung epithelium cells. have investigated effect PBQ on cellular using human type II epithelial cells (A549) also purified tubulin. Cell viability experiments A549 indicated very low IC(50) value (approximately 7.5 microM) for PBQ....
Muscle inflammation is often associated with its expansion. Bladder smooth muscle inflammation-induced cell death accompanied by hyperplasia and hypertrophy as the primary cause for poor bladder function. In mice, DNA damage initiated chemotherapeutic drug cyclophosphamide activated caspase 1 through formation of NLRP3 complex resulting in detrusor hyperplasia. A metabolite, acrolein, caused global methylation accumulation a mouse model cultured cells. correlation, expression was...
2,4-Dichlorophenoxyacetic acid (2,4-D), the most widely used herbicide in world, has been previously reported to induce lung damage. Here this study we have investigated molecular mechanism of 2,4-D induced toxicity A549 and WI38 cell lines. Cell viability experiments indicate IC50 values cells for 72 h are 126 ± 2.25 μM 115 4.39 μM, respectively. Although not arresting a particular phase cycle cells, dose dependently increased subG1 population, indicative death, mode death is apoptosis both...
Periodontal diseases are classified as inflammation affecting the supporting tissue of teeth, which eventually leads to tooth loss. Mild reversible gingivitis and severe irreversible periodontitis most common periodontal diseases. pathogens initiate The bacterial toxin, lipopolysaccharide (LPS), triggers inflammatory response oxidative stress. However, progress stress in is unknown. purpose this study examine cell damage periodontitis. Our results showed that LPS increases reactive oxygen...
Abstract The Michael addition of amines with enones for synthesizing β‐amino carbonyls constitutes a valuable transformation in organic chemistry. While various catalyst have been made available catalyzing the aromatic to but there is no report using α‐amylase enzyme catalyze this transformation. from Aspergillus oryzae was found aryl (hetero) methyl vinyl ketone high catalytic efficiency (63–83% yield). A hybrid copper nanoparticle (α‐amylase@CuNPs) has prepared and used as reusable...
The short half-life in the GI tract necessitates an excess of drugs causing side effects oral formulations. Here, we report development and deployment Bacterioboat, which consists surface-encapsulated mesoporous nanoparticles on metabolically active Lactobacillus reuteri as a drug carrier suitable for administration. Bacterioboat showed up to 16% loading its dry weight, intestinal anchorage around alveoli regions, sustained release, stability physiological conditions 24 hours. In vivo...
Burn injuries are characterized by prolonged inflammatory phases, neurovascular damage, and hypermetabolism, eventually causing improper tissue regeneration. Insulin has gained considerable attention in normal diabetic wound healing, yet its role burn wounds remains poorly understood. In this study, insulin-chitosan nano-formulations (ICNP) were synthesized using a simple robust mechanism to monitor specific interactions between insulin chitosan, the particles measuring approximately 30 nm...
Vitamin K3 (2-methyl-1,4-naphthoquinone), also known as menadione, is the synthetic precursor of all naturally occurring vitamin K in body. necessary for production prothrombin and five other blood-clotting factors humans. We have examined effects menadione on cellular microtubules ex vivo well its binding with purified tubulin vitro. Cell viability experiments using human cervical epithelial cancer cells (HeLa) oral (KB) indicated that IC50 values are 25.6 ± 0.6 64.3 0.36 μM, respectively,...
Paclitaxel (Tx) is one of the first-line chemotherapeutic drugs used against lung cancer, but acquired resistance to this drug a major challenge successful chemotherapy. In work, we have focused on chronological changes various cellular parameters and associated effect Tx (10 nM) development in A549 cell line. It was observed, at initial stage, death percentage due treatment had increased up 20 days, thereafter, it started declining became completely resistant by 40 days. Expressions βIII...
Cuminaldehyde (CA), a monoterpenoid, preset in many plant sources including cumin, induces reactive oxygen-related damage and death Haemonchus contortus, parasitic worm with an LD50, values of 127.3 ± 7.5, 184.5 12.1 104.1 7.9 μg/mL for adult female, male worms (12 h) L3 larvae, respectively (24 h). Fifty percent inhibition egg hatching (IC50) was obtained at 142.4 11.4 after 48 h exposure. Scanning electron microscopy revealed physical to the anterior posterior ends, intestinal, ovarian,...