A5028199325- Breast Cancer Treatment Studies
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Breast Lesions and Carcinomas
- Reproductive Biology and Fertility
- HER2/EGFR in Cancer Research
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Cancer Cells and Metastasis
- Advanced Breast Cancer Therapies
- Cancer Genomics and Diagnostics
- Meat and Animal Product Quality
- Estrogen and related hormone effects
- Radiomics and Machine Learning in Medical Imaging
- Salivary Gland Tumors Diagnosis and Treatment
- Effects and risks of endocrine disrupting chemicals
- Cancer Diagnosis and Treatment
- Studies on Chitinases and Chitosanases
- Global Cancer Incidence and Screening
- Protein Hydrolysis and Bioactive Peptides
- Veterinary Equine Medical Research
- Economic and Financial Impacts of Cancer
- Breast Implant and Reconstruction
- RNA Interference and Gene Delivery
- Cancer survivorship and care
Akron General Medical Center
2015-2023
Cleveland Clinic
1987-2022
Doctors Hospital at Renaissance
2019-2021
American College of Surgeons
2020
Indiana University – Purdue University Indianapolis
2017
Indiana University School of Medicine
2017
Mountain Blue Cancer Care Center
2017
Tufts Medical Center
2001
Tufts University
1992-1997
University of Florida
1987-1989
The nonsteroidal estrogenic compound bisphenol A (BPA) is a monomer used in the manufacture of polycarbonate plastics and resins. BPA may be ingested by humans as it reportedly leaches from lining tin cans into foods, dental sealants saliva, bottles their contents. Because weakly estrogenic--approximately 10,000-fold less potent than 17beta-estradiol--current environmental exposure levels have been considered orders magnitude below dose required for adverse effects on health. Herein we...
Hormone receptor-positive (HR+) tumors have heterogeneous biology and present a challenge for determining optimal treatment. In the Neoadjuvant Breast Registry Symphony Trial (NBRST) patients were classified according to MammaPrint/BluePrint subtyping provide insight into response neoadjuvant endocrine therapy (NET) or chemotherapy (NCT).The purpose of this predefined substudy was compare with conventional 'clinical' immunohistochemistry/fluorescence in situ hybridization (IHC/FISH)...
Objective: Single-center studies have demonstrated that resection of cavity shave margins (CSM) halves the rate positive and re-excision in breast cancer patients undergoing partial mastectomy (PM). We sought to determine if these findings were externally generalizable across practice settings. Methods: In this multicenter randomized controlled trial occurring 9 centers United States, stage 0–III PM randomly assigned either CSM (“shave” group) or not (“no shave” group). Randomization...
Published reports have shown that an Mr 90,000−92,000 protein is released into the oviductal lumen of sheep, during estrus at a time corresponding to ovulation and fertilization, where it associates with embryo. The objectives this study were (a) determine whether estradiol-17 β (E) alone or in combination progesterone (P) induces synthesis from ampulla and/or isthmus oviduct; (b) monitor structural alterations epithelial cells associated protein; (c) generate polyclonal antiserum use verify...
An estrogen (E2)-dependent 90,000-92,000 M(r) protein is synthesized and released by the sheep oviduct in a temporally regionally specific manner during first few days of pregnancy, where it associates with embryo. The present study was undertaken to further define nature this its regulation at time when fertilization early embryonic cleavage occur oviduct. complete complementary DNA (cDNA) sequence for determined, steady state messenger RNA (mRNA) levels were measured pregnancy (estrus 1,...
Administration of estradiol-17 β (E) to ovariectomized (ovx) sheep results in the synthesis and release an Mr 90,000−92,000 glycoprotein into oviductal lumen culture medium ampullar explants (Biol Reprod 1992; 47:889–902). The objective this study was determine when from what region oviduct is synthesized released during early pregnancy. Estrous ewes were bred intact rams known fertility, oviducts obtained at estrus (Day 0) Days 1.5, 2, 3, 4, 6, 16 Pregnancy verified by presence a fertilized...
The 80-gene molecular subtyping signature (80-GS) reclassifies a proportion of immunohistochemistry (IHC)-defined luminal breast cancers (estrogen receptor-positive [ER+], human epidermal growth factor receptor 2-negative [HER2-]) as Basal-Type. We report the association 80-GS reclassification with neoadjuvant treatment response and 5-year outcome in patients cancer.Neoadjuvant Breast Registry Symphony Trial (NBRST; NCT01479101) is an observational, prospective study that included 1,069...
The prospective Neoadjuvant Breast Symphony Trial (NBRST) study found that MammaPrint/BluePrint functional molecular subtype is superior to conventional immunohistochemistry/fluorescence in situ hybridization subtyping for predicting pathologic complete response (pCR) neoadjuvant chemotherapy. purpose of this substudy was determine if the rate pCR affected by tumor size.The NBRST includes breast cancer patients who received classified into four subgroups: Luminal A, B, HER2 (human epidermal...
Primary small cell neuroendocrine carcinoma of the breast (SCNCB) is a very uncommon type cancer. Histology and morphology are virtually indistinguishable from carcinomas lung (SCNCL), mandating search for primary site elsewhere in body. There no standard approach to treatment as there only limited number cases reported literature. This report summarizes case SCNCB presents review literature.A 65-year-old female presented her gynecologist with right mass. After biopsy, she was diagnosed...
The biosynthetic activity of the sheep ampulla oviduct changes during first few days pregnancy. An estrogen (E2)-dependent glycoprotein (Mr 90 000𢄡92 000) is expressed, synthesized, and released when fertilization embryo development occur in oviduct. objective present study was to determine whether secretory epithelial lining ampulla, cellular source E2- dependent protein, undergoes morphological alterations early pregnancy a contemporaneous manner. Ampulla oviducts obtained from...
The development of epithelial cells the uterine glands ovariectomized sheep in response to estradiol-17β (E) and progesterone (P) was studied using light electron microscopy. Animals that had been for six weeks were placed one three experimental treatment groups. Group I animals (untreated controls) received no steroid treatment. II (E alone) 4-cm E implant ≈ 5–10 pg/ml) their uteri removed after 2, 4, or 6 days. III (E-primed, P-treated) an days then treated with 13-cm P implants (P 1.5–3...
ABSTRACT: A high‐molecular‐weight (MW > 660,000), acidic glycoprotein (HMWGP) was purified from incubation medium of preimplantation, elongating ovine (day 16 and 17) porcine 16) conceptuses. HMWGP tested for its ability to inhibit [ 3 H‐methyl]thymidine incorporation into lymphocytes stimulated by phytohemagglutinin or two‐way mixed lymphocyte cultures. Ovine inhibited the in a dose‐dependent manner. An approximately 50% inhibition detected at lowest dose (ovine, 25 μg/ml; porcine, 5...
Abstract Oviducts and uteri were obtained from cats at estrus up to 14 days after coitus. Progesterone binding sites estimated by Scatchard analysis, serum levels of progesterone determined radioimmunoassay, key morphological parameters the lining epithelium oviduct deep uterine glands quantitated. cytosol was hightest estrus, significantly depleted day 4, continued a gradual decline out in both tissues. Nuclear markedly elevated 4; with time decreased until it equal or lower than detected...
The nonsteroidal estrogenic compound bisphenol A (BPA) is a monomer used in the manufacture of polycarbonate plastics and resins. BPA may be ingested by humans as it reportedly leaches from lining tin cans into foods, dental sealants saliva, bottles their contents. Because weakly estrogenic--approximately 10,000-fold less potent than 17beta-estradiol--current environmental exposure levels have been considered orders magnitude below dose required for adverse effects on health. Herein we...
An estrogen-dependent polypeptide (CUPED), which was purified from uterine flushings of estrogen-treated cats, localized in endometrial epithelial cells cats using the peroxidase-antiperoxidase immunocytochemical staining procedure. Epithelial animals treated with estradiol for 4, 7, or 14 days and estrogen-primed progesterone 2 showed positive immunostaining. Staining absent untreated ovariectomized 4 days. Specific cytoplasmic confined to apical secretory granules nonciliated deep glands....
An estrogen-dependent secretory protein (CUPED) was detected and purified from uterine flushings of ovariectomized cats treated with 17 beta-estradiol. The not in obtained untreated animals or estrogen-primed progesterone for 4 days. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed the presence 1 2 bands relative mobility values less than reduced denatured thyroglobulin (Mr = 330,000). by differential centrifugation filtration chromatography. Antiserum raised against this...
Abstract Background The Neoadjuvant Breast Symphony Trial (NBRST) demonstrated the 70-gene risk of distant recurrence signature, MammaPrint, and 80-gene molecular subtyping BluePrint, precisely determined preoperative pathological complete response (pCR) in breast cancer patients. We report 5-year follow-up results addition to an exploratory analysis by age menopausal status. Methods observational, prospective NBRST (NCT01479101) included 954 early-stage patients aged 18–90 years who...
The luminal environment of the estrogen (E2)-dominated mammalian oviduct generates and sustains in which first embryonic cleavages take place. objective this study was to determine, by use an antiserum against E2-dependent sheep secretory glycoprotein (Mr 90,000–92,000), whether E2-dominated pregnant Djungarian hamster (Phodopus sungorus) releases antigenically related protein. If protein present, a secondary define its fate association with filamentous-actin (f-actin) chromatin patterns...
521 Background: Hormone receptor positive (HR+), HER2- early stage breast cancer (ESBC) is a heterogeneous disease that has shown lower response to neoadjuvant chemotherapy (NCT) compared with other clinicopathologic subtypes. Genomic profiling may help inform treatment decisions for ESBC by predicting likelihood of pathological complete (pCR) or chemosensitivity. The 70-gene MammaPrint (MP) test classifies patients as having Low High Risk distant metastasis. In the ISPY2 trial, further...
Abstract Uteroferrin, a progesterone-induced secretory protein of the pig uterus, can noncovalently associate with additional glycoproteins (uteroferrin-associated or UfAP) to form heterodimer. The UfAP were dissociated from uteroferrin by passage through an immunoaffinity column. flow material consisted two immunologically related variants different size (Mr = 47,000-50,000 and Mr 39,000-40,000) forms. By using antiserum all molecular weight components UfAP, it was shown that these...