A5012549909- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Aortic aneurysm repair treatments
- Advanced Polymer Synthesis and Characterization
- Tissue Engineering and Regenerative Medicine
- Aortic Disease and Treatment Approaches
- Infectious Aortic and Vascular Conditions
- Surgical site infection prevention
- Bone fractures and treatments
- Cancer, Hypoxia, and Metabolism
- DNA and Nucleic Acid Chemistry
- Reconstructive Surgery and Microvascular Techniques
- Connective tissue disorders research
- Dendrimers and Hyperbranched Polymers
- Polymer Surface Interaction Studies
- Virus-based gene therapy research
- Pituitary Gland Disorders and Treatments
- CRISPR and Genetic Engineering
- Cardiac Fibrosis and Remodeling
- Cardiac, Anesthesia and Surgical Outcomes
- Pluripotent Stem Cells Research
- Hydrogels: synthesis, properties, applications
- Macrophage Migration Inhibitory Factor
- Signaling Pathways in Disease
- Hedgehog Signaling Pathway Studies
Medical University of Silesia
2017-2024
Novel amino-functionalized 2-oxazoline-based copolymers able to form complexes with DNA were obtained. Their efficiency of condensation into polyplexes scales appropriate for effective cellular internalization was studied.
Novel, nontoxic star copolymers of N,N-dimethylaminoethyl methacrylate (DMAEMA) and hydroxyl-bearing oligo(ethylene glycol) (OEGMA-OH) were synthesized via atom transfer radical polymerization (ATRP) using hyperbranched poly(arylene oxindole) as the macroinitiator. Stars with molar masses from 100,000 g/mol to 257,000 various amounts OEGMA-OH in arms prepared. As these polymers can find applications, e.g., carriers nucleic acids, drugs or antibacterial antifouling agents, this work, much...
Introduction: The benefits of patient’s specific cell/gene therapy have been reported in relation to numerous genetic related disorders including osteogenesis imperfecta (OI). In particularly also a drug based on the administration bisphosphonates partially helped ease symptoms. Methods: this controlled trial, fibroblasts derived from patient diagnosed with OI type II successfully reprogrammed into induced Pluripotent Stem cells (iPSCs) using Yamanaka factors. Those were subjected repair...
Star polymers of N,N′-dimethylaminoethyl methacrylate (DMAEMA) and hydroxyl-bearing oligo(ethylene glycol) (OEGMA-OH) were used as pDNA mRNA carriers. To the best our knowledge, this is first report stars in formation polyplexes with mRNA. Polyplexes efficiently delivered nucleic acids into HT-1080 cells, while their cytotoxicity was kept significantly low due to presence OEGMA-OH units star. Hydroxyl functionalities for introduction fluorophore moieties monitoring complex location after...
Star polymer nanolayers were successfully used as surfaces for fibroblast adhesion and proliferation, followed by their detachment in the form of a cell sheet, controlled temperature decrease.
Abstract Adamantinomatous craniopharyngioma (ACP) is an intracranial tumor considered partly malignant due to its ability infiltrate surrounding structures and tendency relapse despite radical resection. CD44 a known stem cell marker in ACP upregulated clusters of invasive protrusions; however, the functions alternative splicing isoform variants, CD44s CD44v1-10, have not yet been studied terms recurrence, their confirmed roles cancer development progression. In this study, we first...
In this work, we studied the stability of matrices with temperature-dependent solubility and their interactions water at physiological temperature for application in cell culture vitro. Gradient copolymers 2-isopropyl- 2-n-propyl-2-oxazoline (P(iPrOx-nPrOx)) were used to prepare matrices. The comonomer ratio during polymerization was chosen such that cloud point (TCP) copolymer below 37 °C while glass transition (Tg) above °C. role support context aqueous solution examined. Therefore, form...
Abstract Abdominal aortic aneurysm refers to abnormal, asymmetric distension of the infrarenal wall due pathological remodelling extracellular matrix. The distribution enzymes matrix and their expression patterns in affected tissue are largely unknown. goal this work was investigate profiles 20 selected genes coding for metalloproteinases inhibitors proximal distal direction abdominal aneurysm. RNA samples were purified from four lengthwise fragments border obtained 29 patients. quantities...
Ischemic heart disease (IHD) is one of the main focuses in today’s healthcare due to its implications and complications, it predicted be increasing prevalence ageing population. Although conventional pharmacological interventional methods for treatment IHD presents with success clinical setting, long-term complications cardiac insufficiency are on a continual incline as result post-infarction remodeling tissue. The migration involvement stem cells muscle, followed by differentiation into...
The aim of the study was to investigate specific potential markers for cells obtained from three layers human AAA divided into segments along based on morphological differences. isolated were compared control commercial cell types healthy abdominal aortas. For each type aortic layer, specimens 6 patients compared. Total RNA 36 cultures gene expression profiling and new cytometry typed. Isolated analyzed by flow using fluorochrome-conjugated antibodies markers: CNN1, MYH10, ENG, ICAM2, TEK....
Apoptosis is a widely controlled, programmed cell death, defects in which are the source of various diseases such as neurodegenerative well cancer. The use apoptosis therapy human increasing interest, and analysis factors involved its regulation valuable designing specific carriers capable targeting death. Highly efficient precisely controlled delivery genetic material by low-toxic one most important challenges apoptosis-based gene therapy. In this work, we investigate effect star polymer...
Median sternotomy is the surgical method of choice for many procedures where one main problems long post-operative wound healing process leading to sternal dehiscence and development infection. This leads prolonged hospital stay increased mortality due complications. A promising solution seems be use allogeneic chondrocytes treatment, whose properties in field cartilage reconstruction are widely used medicine, mainly orthopedics. In present study, we investigated effect local delivery on...
Stem cell treatment is a promising method of therapy for the group patients whose conventional options have been limited or rejected. cells potential to repair, replace, restore and regenerate cells. Moreover, their proliferation level high. Owing these features, they can be used in numerous diseases, such as cancer, lung diseases ischemic heart diseases. In recent years, stem has greatly developed, shedding light on stromal-derived factor 1α (SDF-1α). SDF-1α mobilizing chemokine application...
Introduction: This study aimed to standardize cell culture methods for major types isolated from three layers of human AAA. We also determine in each layer AAA segment and compare them with control, unchanged segments. Material methods: divided AAAs into segments along the control flanking aneurysm. Isolated cells following expansion were analyzed by flow cytometry, immunochemistry, microscopic methods. Fluorochrome-conjugated antibodies used detect (endothelial cells, smooth muscle...
Abstract The median sternotomy is the incision of choice for many procedures where one major problems long post-operative wound healing process leading to sternal dissection and development infection. This leads prolonged hospital stay increased mortality from complications. A promising solution appears be use allogeneic chondrocytes treatment, whose properties reconstruction cartilage structures are widely used in medicine, mainly orthopedics. In present study, we investigated effect local...