A5101986617- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
- Chromatin Remodeling and Cancer
- Invertebrate Immune Response Mechanisms
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Genetics and Neurodevelopmental Disorders
- Neurobiology and Insect Physiology Research
- Marine Ecology and Invasive Species
- RNA and protein synthesis mechanisms
- Insect and Arachnid Ecology and Behavior
- Protist diversity and phylogeny
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Marine Invertebrate Physiology and Ecology
- Physiological and biochemical adaptations
- Renal and related cancers
- Reproductive biology and impacts on aquatic species
- Genetics, Aging, and Longevity in Model Organisms
- Animal Behavior and Reproduction
University of Tsukuba
2015-2025
Nagoya University
2012-2024
Japan Tobacco (Japan)
2024
New York Institute of Technology
2022
Hiroshima University
2021
Thermo Fisher Scientific (Japan)
2017-2018
Dokkyo Medical University
2007-2018
The Graduate University for Advanced Studies, SOKENDAI
2005-2017
Okazaki Institute for Integrative Bioscience
2007-2017
National Institute for Basic Biology
2008-2017
In Drosophila , maternally supplied Nanos functions in the migration of primordial germ cells (PGCs) into gonad; mice, zygotic genes are involved instead. We report cloning and functional analyses nanos2 nanos3 mice. These differentially expressed mouse PGCs. is predominantly male cells, elimination this gene results a complete loss spermatogonia. However, found migrating PGCs, factor both sexes. Hence, although mice flies differ their mechanisms for cell specification, there seems to be...
Embryonic patterning in Drosophila is regulated by maternal factors. Many such factors become localized as mRNAs within the oocyte during oogenesis and are translated a spatio-temporally manner. These processes controlled trans-acting proteins, which bind to target RNAs form ribonucleoprotein (RNP) complex. We report that DEAD-box protein, Me31B, forms cytoplasmic RNP complex with oocyte-localizing Exuperantia, protein involved RNA localization. During early oogenesis, loss of Me31B causes...
ABSTRACT A subtractive hybridization strategy was used to isolate putative genes involved in the development of mouse primordial germ cells (PGC). Complimentary DNA amplified on RNA isolated from base allantois where PGC are located 7.5 days post coitum (dpc) embryo. It then subtracted by with cDNA anterior region absent. novel gene thus is designated as Mesp1 and encodes a possible transcription factor MesP1 containing basic helix-loop-helix motif. Its earliest expression observed at onset...
In Drosophila embryos, germ cell formation is induced by specialized cytoplasm at the posterior of egg, pole plasm. Pole plasm contains polar granules, organelles in which maternally produced molecules required for are assembled. An untranslatable RNA, called Polar granule component ( Pgc ), was identified and found to be localized granules. Most cells embryos transgenic females expressing antisense RNA failed complete migration populate embryonic gonads, that developed from these often had...
In insects, the precise timing of molting and metamorphosis is strictly guided by a principal steroid hormone, ecdysone. Among multiple conversion steps for synthesizing ecdysone from dietary cholesterol, 7-dehydrocholesterol to 5beta-ketodiol, so-called 'Black Box', thought be important rate-limiting step. Although number genes essential synthesis have recently been revealed, much less known about that are crucial functioning in Black Box. Here we report on novel ecdysteroidgenic gene,...
Stem cells are maintained in vivo by short-range signaling systems specialized microenvironments called niches, but the molecular mechanisms controlling physical space of stem cell niche poorly understood. In this study, we report that heparan sulfate (HS) proteoglycans (HSPGs) essential regulators germline (GSC) niches Drosophila melanogaster gonads. GSCs were lost both male and female gonads mutants deficient for HS biosynthesis. dally, a glypican, is expressed GSC responsible maintaining...
Highlights•Mouse spermatogenic stem cells (SSCs) migrate among their differentiating progeny•Lymphatic endothelial near vasculature secrete FGFs that act as SSC mitogens•SSCs tune self-renewal and differentiation in response to FGF consumption•Competition for limited supply of mitogen (FGFs) regulates density homeostasisSummaryIn many tissues, homeostasis is maintained by physical contact between an anatomically defined niche. However, how cell achieved environments where are motile...
How germ cells become sperm or egg During vertebrate development, switch from a sexually indifferent to committed state for either sperm. Signals somatic gonadal are generally thought influence the sexual differentiation of cells. However, Nishimura et al. demonstrate that cell–intrinsic sex determination cues at play in teleost fish medaka. The forkhead box transcriptional factor foxl3 represses initiation spermatogenesis. In absence function, females develop ovaries filled with functional...
Mitochondrial large ribosomal RNA (mtlrRNA) has been identified as a cytoplasmic factor that induces pole cell formation in embryos whose ability to form germ line abolished by treatment with ultraviolet light. In situ hybridization analyses reveal mtlrRNA is enriched plasm and tightly associated polar granules, the distinctive organelles of plasm, which supports idea functions formation. This suggests product from mitochondrial genome, along nuclear products, participates key event...
Nanos (Nos) is one of the evolutionarily conserved proteins known to direct germ-line development. In Drosophila , maternal Nos protein maintains transcriptional quiescence in progenitors or pole cells repress ectopic expression somatic genes. Here we show that required establish and maintain identity by preventing apoptosis cell fate. The lacking were degraded during mid late embryogenesis. When was suppressed Df(3L)H99 some adopted fates. These expressed markers ectopically lost marker...
The planarian's remarkable regenerative ability is thought to be supported by the stem cells (neoblasts) found throughout its body. Here we report identification of a subpopulation neoblasts, which was revealed expression nanos ‐related gene planarian Dugesia japonica , termed Djnos . ‐expressing in asexual were distributed prospective ovary or testes forming region sexual planarian. During sexualization, produce germ cells, suggesting that state these kept as germline for oogonia and...
The transcription factor GATA-4 protects cardiomyocytes against doxorubicin-induced cardiotoxicity. Here, we report the identification of Bcl2 as a direct target gene GATA4 that may mediate prosurvival function in cardiomyocytes. transcript and protein levels were reduced by doxorubicin neonatal rat ventricular (NRVC) mouse heart determined RT-PCR Western blot analysis. reduction was prevented overexpression NRVC transgenic heart. Also, expression increased baseline 30% 2.7-fold heart,...
To preserve genome integrity, an evolutionarily conserved small RNA-based silencing mechanism involving PIWI proteins and PIWI-interacting RNAs (piRNAs) represses potentially deleterious transposons in animals. Although there has been extensive research into bilaterians, these remain to be examined ancient phyla. Here, we investigated the Hywi Hyli cnidarian Hydra, found that both are enriched multipotent stem cells, germline female germline. localize nuage, a perinuclear organelle...
Many animals have an intrinsic growth checkpoint during juvenile development, after which irreversible decision is made to upregulate steroidogenesis, triggering the metamorphic juvenile-to-adult transition. However, a molecular process underlying such critical developmental remains obscure. Here we show that nutrient-dependent endocycling in steroidogenic cells provides machinery necessary for activation of metamorphosis Drosophila melanogaster. Endocycle progression prothoracic gland (PG)...
Abstract Accumulating evidence suggests that exosomes are heterogeneous in molecular composition and physical properties. Here we examined whether epithelial cells secrete a population of exosomes, if is the case, cell polarity affects release different populations especially those carrying Wnt. Sucrose-density ultracentrifugation marker analysis revealed or exosome-like vesicles were released from MDCK depending on polarity. Wnt3a associated with these detectable culture media collected...
Nanos (Nos) is an evolutionarily conserved protein essential for the survival of primordial germ cells. In Drosophila , maternal Nos partitions into pole cells and suppresses apoptosis to permit proper germ-line development. However, how this critical event regulated by has remained elusive. Here, we report that represses suppressing translation head involution defective ( hid ), a member RHG gene family required Caspase activation. addition, demonstrate acts in concert with another gene,...
ABSTRACT Screening a cDNA library generated from poly(A)+RNA of Drosophila cleavage embryos, we selected clone (pDE20.6). The hybridized specifically with that is capable restoring embryos u.v.-caused inability pole cell formation. RNA hybrid-selected by pDE20.6 was also able to induce cells in the anterior region if it coinjected u.v.-irradiated polar plasm, although or irradiated plasm alone not effective. Pole thus formed posterior were identified granules and nuclear bodies,...
Germ-line stem cells (GSCs) are maintained by the somatic microenvironment, or GSC niche, which ensures that GSCs can both self-renew and produce functional gametes. However, it remains unclear how proper niche size location regulated within developing gonads. In Drosophila testis, hub form derived from a subset of gonadal precursors (SGPs) in anterior portion embryonic gonad. Here we show Notch signaling induces differentiation. is activated almost all SGPs male gonad, but Epidermal growth...