Mycobacterium abscessus is the most pathogenic species among predominantly saprophytic fast-growing mycobacteria. This opportunistic human pathogen causes severe infections that are difficult to eradicate. Its ability survive within host was described mainly with rough (R) form of M . , which lethal in several animal models. R not present at very beginning disease but appears during progression and exacerbation mycobacterial infection, by transition from a smooth (S) form. However, we do...

ABSTRACT Inactivation of Simian Virus 40 large T antigen, in cells immortalized with conditional mutants, leads to activation p53 and apoptosis. We used the mRNA differential display method identify genes differentially expressed during this process. found that steady-state levels for cytoplasmic actins decreased early also showed that, although level corresponding proteins is not profoundly affected, they are substrates an interleukin 1-β converting enzyme (ICE)-like protease activated...

The retinoblastoma gene, rb, ensures at least its tumor suppressor function by inhibiting cell proliferation. Its role in apoptosis is more complex and less described than cycle regulation. Rbf1, the Drosophila homolog of Rb, has been found to be pro-apoptotic proliferative tissue. However, way it induces molecular level still unknown. To decipher this mechanism, we induced rbf1 expression wing We that Rbf1-induced depends on dE2F2/dDP heterodimer, whereas dE2F1 transcriptional activity not...

Objectives The human leucocyte antigen (HLA)-B27 confers an increased risk of spondyloarthritis (SpA) by unknown mechanism. objective this work was to uncover HLA-B27 non-canonical properties that could explain its pathogenicity, using a new Drosophila model. Methods We produced transgenic expressing the SpA-associated HLA-B*27:04 or HLA-B*27:05 subtypes, non-associated HLA-B*07:02 allele, alone in combination with β2-microglobulin (hβ2m), under tissue-specific drivers. Consequences...

ABSTRACT The Drosophila ovo gene, which encodes a putative transcription factor (Ovo) with TFIIIA-like zinc fingers, is required for female germline survival and proper oogenesis. Three dominant female-sterile ovoD mutations cause ovarian abnormalities that define an allelic series, ovoD1 displaying the stronger phenotype ovoD3 weaker. We report here all three are point create new in-frame methionine codons in 5′ part of ovo. There two types over-lapping units, ovoα ovoβ. By using various...

In accordance with its tumor suppressor role, the Retinoblastoma protein pRb can ensure pro-apoptotic functions. rbf1, Drosophila homolog of Rb, also displays a activity in proliferative cells. We previously showed that rbf1 depends on ability to decrease level anti-apoptotic proteins such as Bcl-2 family Buffy. Buffy often acts opposite Debcl, other Bcl-2-family protein. Both localize at mitochondrion, but way they control apoptosis still remains unclear. Here, we demonstrate debcl and...

Members of the Bcl-2 family are key elements apoptotic machinery. In mammals, this multigenic contains about twenty members, which either promote or inhibit apoptosis. We have previously shown that mammalian pro-apoptotic member Bax is very efficient in inducing apoptosis Drosophila, allowing study bax-induced cell death a genetic animal model. report here results screening P[UAS]-element insertion library performed to identify gene products modify phenotypes induced by expression bax...

The Jun Kinase (JNK) signaling pathway responds to diverse stimuli by appropriate and specific cellular responses such as apoptosis, differentiation or proliferation. mechanisms that mediate this specificity remain largely unknown. core of pathway, composed a JNK protein kinase (JNKK), can be activated various putative JNKK kinases (JNKKK) which are themselves downstream different adaptor proteins. A proposed hypothesis is the response lies in combination JNKKK an upstream JNKK. We...

Mycobacterium abscessus, an intracellular nontuberculous mycobacterium, is considered the most pathogenic species among group of rapidly growing mycobacteria. The resistance M. abscessus to host innate response contributes its pathogenicity in addition several virulence factors. We have recently shown Drosophila that antimicrobial peptides (AMPs), whose production induced by are unable control mycobacterial infection. This could be due their inability kill mycobacteria and/or hidden location...

Neural stem cells (NSCs) reside in a defined cellular microenvironment, the niche, which supports generation and integration of newborn neurons. The mechanisms building sophisticated niche structure around NSCs their functional relevance for neurogenesis are yet to be understood. In Drosophila larval brain, cortex glia (CG) encase individual NSC lineages membranous chambers, organising cell population neurons into stereotypic structure. We first found that CG wrap lineage-related regardless...

The retinoblastoma protein, pRb, plays important roles in many processes implicated cell fate decisions, including cycle, differentiation and apoptosis. In cycle regulation, pRb interacts principally with the E2F transcription factor family members to inhibit of genes controlling progression. this study, we focused on role apoptosis, which is much less clear than its regulation. Indeed, has been found be either pro- or anti-apoptotic. To clarify how proliferative status cells impacts used...

Apoptosis is associated with numerous phenotypical characteristics, and thus studied many tools. In this study, we compared two broadly used apoptotic assays: TUNEL staining an antibody targeting the activated form of effector caspase. To compare them, developed a protocol based on commonly tools such as image filtering, z-projection, thresholding. Even though it in image-processing protocols, thresholding remains recurring problem. Here, analyzed impact processing parameters readout choice...

DREAM complexes are transcriptional regulators that control the expression of hundreds to thousands target genes involved in cell cycle, quiescence, differentiation, and apoptosis. These contain many subunits can vary according considered genes. Depending on their composition nature partners they recruit, gene through diverse mechanisms, including chromatin remodeling, transcription cofactor factor recruitment at various genomic binding sites. This complexity is particularly high mammals....

The tumor suppressor retinoblastoma protein (pRb) is inactivated in a wide variety of cancers. While its role during cell cycle well characterized, little known about properties on apoptosis regulation and apoptosis-induced responses. pRb shorter forms that can modulate pRB apoptotic properties, resulting from cleavages at caspase specific sites are observed several cellular contexts. A bioinformatics analysis showed putative cleavage site (TELD) found the Drosophila homologue (RBF) position...