Exosomes, nanoscale extracellular vesicles functioning as cell-to-cell communicators, are an emerging promising therapeutic in the field of bone tissue engineering. Here, we report construction and evaluation a novel cell-free tissue-engineered that successfully accelerated restoration critical-sized mouse calvarial defects through combining exosomes derived from human adipose-derived stem cells (hASCs) with poly(lactic-co-glycolic acid) (PLGA) scaffolds. The were immobilized on...

Abstract Objectives The present study aimed to investigate whether exosomes derived from miR‐375‐overexpressing human adipose mesenchymal stem cells (hASCs) could enhance bone regeneration. Materials and Methods Exosomes enriched with miR‐375 (Exo [miR‐375]) were generated hASCs stably overexpressing after lentiviral transfection identified transmission electron microscopy, nanosight western blotting. construction efficiency of Exo (miR‐375) was evaluated qRT‐PCR incubated marrow (hBMSCs)...

Human adipose-derived stem cells (hASCs) are multipotent progenitor with self-renewal capabilities and multilineage differentiation potential, including osteogenesis. Although protein deubiquitinases have been linked to cell fate determination, whether deubiquitination contributes lineage commitment during osteogenic of hASCs remains be investigated. The objective this study was evaluate the effects ubiquitin specific protease 7 (USP7) on hASCs. An osteocalcin promoter driven luciferase...

The stability of soft and hard tissues surrounding the implant is not only a matter aesthetics, but also affects long-term implant. present study was to explore influence buccal mucosa width/height (W/H) ratio, emergence profile bone width on peri-implant tissue changes in posterior region. Fifty-eight restoration cases were recruited this study. Evaluations performed at time placement (T0), 1 year later (T1). Buccal (BMW), (BBW), inclination angle, angle evaluated. variables that may affect...

Short implants (intra-bony length ≤ 8 mm) are generally considered as an alternative to bone augmentation in challenging situations; however, clinical evidence from large-scale studies with long follow-up regarding the application of short remains deficient.The present study aimed assess mid-term outcomes supporting fixed prostheses posterior region, and investigate effects crown-to-implant ratio (C/I), other patient-, implant-, prosthesis-relevant factors on conditions around implants.180...

Abstract Background The prosthetic emergence profile design might be an important factor in postsurgical mucosal recession etiology. Therefore, a restorative buccal designed correctly reduce gingival margin recession. Purpose To compare the marginal level and width/height (W/H) ratio between two configurations of single implant‐supported restorations at molar sites. Materials Methods Twenty‐one patients requiring mandibular tooth replacement with supracrestal thickness ≥2 mm were recruited...

Purpose: To evaluate the implant survival and prevalence of biologic mechanical complications in edentulous patients restored with complete-arch implant-supported fixed dental prostheses (IFDPs). Materials Methods: Patients screw-retained IFDPs between January 2012 December 2019 a minimum 2-year follow-up were included. Outcome measures cumulative rate (CSR) for implants prostheses, complications, complications. A generalized estimating equation model was used to estimate potential risk...

GATA3 is a key transcription factor in cell fate determination and its dysregulation has been implicated various types of malignancies. However, how the abundance function are regulated remains unclear. Here, we report that physically associated with FBXW7α, FBXW7α destabilizes through assembly SKP1‐CUL1‐F‐box E3 ligase complex. Importantly, showed promotes ubiquitination degradation GSK3 dependent manner. Furthermore, demonstrated inhibits breast cancer cells survival destabilizing GATA3,...

Abstract Background Lamina-associated polypeptide 2α (LAP2α) is a nucleoplasmic protein that has been involved in the regulation of cell cycle, gene transcription, and adult stem function. LAP2α down-regulation linked to age-related osteoporosis bone deformities; however, underlying mechanisms remain obscure. The present study aimed elucidate function osteogenic differentiation human adipose-derived cells (hASCs), which are attractive sources for tissue engineering. Methods expression during...

To compare clinical and histological outcomes of sinus augmentation performed immediately or 3 months after pseudocyst removal through a prospective randomized controlled study.In total, 33 procedures were in 31 patients. Augmentation was either (one-stage intervention) (two-stage intervention). Six postoperatively, bone specimens harvested, histomorphometric analysis as primary outcome. Data recorded evaluated for implant survival rates, marginal resorption, complication rate,...

Proteins containing breast cancer type 1 (BRCA1) C-terminal domains play crucial roles in response to and repair of DNA damage. Epithelial cell transforming factor (epithelial sequence 2 [ECT2]) is a member the BRCA1 protein family, but it not known if ECT2 directly contributes repair. In this study, we report that recruited lesions poly (ADP-ribose) polymerase 1-dependent manner. Using co-immunoprecipitation analysis, showed physically associates with KU70-KU80 BRCA1, proteins involved...

Onlay bone grafting is considered highly reliable for reconstructing severe horizontal defects. A critical problem how to achieve precise position of the block control alveolar ridge dimensions. This research aims establish a digital workflow prosthetically oriented onlay and evaluate its accuracy efficiency.This prospective pilot study investigated eight patients who required implant restoration in esthetic area with The includes preoperative virtual planning, design manufacture...

Adipogenesis, a pivotal cellular process involving the differentiation of mesenchymal stem cells (MSCs) to mature adipocytes, plays significant role in various physiological functions. Dysregulation adipogenesis is implicated conditions such as obesity. However, complete molecular understanding remains elusive. This study aimed uncover novel lamina-associated polypeptide 2 alpha (LAP2α) human adipose-derived (hASCs) and its impact on high-fat diet (HFD)-induced obesity associated metabolic...

BACKGROUND The extraction of maxillary impacted teeth is a common procedure in oral surgery, frequently complicated by oroantral communications. For less-experienced clinicians, accurately assessing the difficulty and associated risks third molar extractions remain significant challenge. CASE SUMMARY We present case involving disparate outcomes following bilateral molars. Using cone-beam computed tomography three-dimensional software, we conducted digital assessment factors contributing to...

To identify the impact of residual bone height on 5-year implant survival and prosthetic complication rates in patients who underwent maxillary sinus grafting.A total 87 consecutive were treated with 104 lateral approach floor augmentation procedures 100% deproteinized bovine received 169 implants. The analysis considered patient age, sex, time placement, height. Patients < 3 mm assigned to study group; otherwise, they placed control group.The mean follow-up was 68.2 months (0 103 months)....